The Molecular Dimensions of Mitogenic Factor from Guinea Pig Lymph Node Cells
The molecular dimensions of a mitogenic factor from guinea pig lymph node cells were investigated by means of gel filtration, sucrose density gradient ultracentrifugation, and CsCi isopycnic ultracentrifugation. The mitogenic factor was produced by stimulation of immune lymph node cells from inbred...
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| Veröffentlicht in: | The Journal of immunology (1950) 1975-01, Vol.114 (1 Part 1), p.10-16 |
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| container_issue | 1 Part 1 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 114 |
| creator | Gately, Celia L Gately, Maurice K Mayer, Manfred M |
| description | The molecular dimensions of a mitogenic factor from guinea pig lymph node cells were investigated by means of gel filtration, sucrose density gradient ultracentrifugation, and CsCi isopycnic ultracentrifugation. The mitogenic factor was produced by stimulation of immune lymph node cells from inbred guinea pigs, strain 2 or 13, with the specific antigen, ovalbumin; and mitogenic activity was assayed by measuring incorporation of tritiated thymidine by target lymph node cells from syngeneic animals not immune to ovalbumin. The D-20,w of the mitogenic factor was estimated to be (9.9 plus and minus 0.2) times 10-7 cm2/sec by gel filtration on Sephadex G-100, and the apparent S20,w was estimated to be 2.4 plus and minus 0.2 S by sucrose density gradient ultracentrifugation. The buoyant density was determined to be 1.324 plus and minus 0.006 g/ml by CsCi isopycnic ultracentrifugation, and from this value the partial specific volume of mitogenic factor was estimated to be 0.71. From these data the molecular weight of the mitogenic factor was calculated to be 20,000, and the frictional ratio was calculated to be 1.2. |
| doi_str_mv | 10.4049/jimmunol.114.1_Part_1.10 |
| format | Article |
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The mitogenic factor was produced by stimulation of immune lymph node cells from inbred guinea pigs, strain 2 or 13, with the specific antigen, ovalbumin; and mitogenic activity was assayed by measuring incorporation of tritiated thymidine by target lymph node cells from syngeneic animals not immune to ovalbumin. The D-20,w of the mitogenic factor was estimated to be (9.9 plus and minus 0.2) times 10-7 cm2/sec by gel filtration on Sephadex G-100, and the apparent S20,w was estimated to be 2.4 plus and minus 0.2 S by sucrose density gradient ultracentrifugation. The buoyant density was determined to be 1.324 plus and minus 0.006 g/ml by CsCi isopycnic ultracentrifugation, and from this value the partial specific volume of mitogenic factor was estimated to be 0.71. From these data the molecular weight of the mitogenic factor was calculated to be 20,000, and the frictional ratio was calculated to be 1.2.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.114.1_Part_1.10</identifier><identifier>PMID: 1112974</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Animals ; Cells, Cultured ; Centrifugation, Density Gradient ; Cesium ; Chromatography, Gel ; Culture Media ; Freund's Adjuvant ; Guinea Pigs ; Immunization ; Lymph Nodes - cytology ; Lymphocytes - immunology ; Mitogens - analysis ; Molecular Conformation ; Sucrose ; Thymidine - metabolism ; Tritium ; Ultracentrifugation</subject><ispartof>The Journal of immunology (1950), 1975-01, Vol.114 (1 Part 1), p.10-16</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-74ed2c28ad090229e96d9c1916c208709c23e205122513f8839cc468495dce993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1112974$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gately, Celia L</creatorcontrib><creatorcontrib>Gately, Maurice K</creatorcontrib><creatorcontrib>Mayer, Manfred M</creatorcontrib><title>The Molecular Dimensions of Mitogenic Factor from Guinea Pig Lymph Node Cells</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>The molecular dimensions of a mitogenic factor from guinea pig lymph node cells were investigated by means of gel filtration, sucrose density gradient ultracentrifugation, and CsCi isopycnic ultracentrifugation. The mitogenic factor was produced by stimulation of immune lymph node cells from inbred guinea pigs, strain 2 or 13, with the specific antigen, ovalbumin; and mitogenic activity was assayed by measuring incorporation of tritiated thymidine by target lymph node cells from syngeneic animals not immune to ovalbumin. The D-20,w of the mitogenic factor was estimated to be (9.9 plus and minus 0.2) times 10-7 cm2/sec by gel filtration on Sephadex G-100, and the apparent S20,w was estimated to be 2.4 plus and minus 0.2 S by sucrose density gradient ultracentrifugation. The buoyant density was determined to be 1.324 plus and minus 0.006 g/ml by CsCi isopycnic ultracentrifugation, and from this value the partial specific volume of mitogenic factor was estimated to be 0.71. From these data the molecular weight of the mitogenic factor was calculated to be 20,000, and the frictional ratio was calculated to be 1.2.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Centrifugation, Density Gradient</subject><subject>Cesium</subject><subject>Chromatography, Gel</subject><subject>Culture Media</subject><subject>Freund's Adjuvant</subject><subject>Guinea Pigs</subject><subject>Immunization</subject><subject>Lymph Nodes - cytology</subject><subject>Lymphocytes - immunology</subject><subject>Mitogens - analysis</subject><subject>Molecular Conformation</subject><subject>Sucrose</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><subject>Ultracentrifugation</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkMtu2zAQRYmiges4_YQCXHVVOTMU9eCycPMC7CSLZE2w1MimIYouKcHI31eFHRTIahb3MReHMY6wlCDV9d55P_ahWyLKJepnEweNS4RPbI5FAVlZQvmZzQGEyLAqqy_sMqU9AJQg5IzNEFGoSs7Z5mVHfBM6smNnIv_lPPXJhT7x0PKNG8KWemf5rbFDiLyNwfO70fVk-LPb8vWbP-z4Y2iIr6jr0hW7aE2X6Ov5Ltjr7c3L6j5bP909rH6uM5urasgqSY2wojYNqGmhIlU2yqLC0gqoK1BW5CSgQCEKzNu6zpW1sqylKhpLSuUL9v3Ue4jhz0hp0N4lOy0wPYUx6VpUWFcSJmN9MtoYUorU6kN03sQ3jaD_kdTvJPVEUr-TnNQp-u38Y_ztqfkfPKGb9B8nfee2u6OLpJM3XTe5UR-Px491fwERmYGI</recordid><startdate>19750101</startdate><enddate>19750101</enddate><creator>Gately, Celia L</creator><creator>Gately, Maurice K</creator><creator>Mayer, Manfred M</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19750101</creationdate><title>The Molecular Dimensions of Mitogenic Factor from Guinea Pig Lymph Node Cells</title><author>Gately, Celia L ; Gately, Maurice K ; Mayer, Manfred M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-74ed2c28ad090229e96d9c1916c208709c23e205122513f8839cc468495dce993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Centrifugation, Density Gradient</topic><topic>Cesium</topic><topic>Chromatography, Gel</topic><topic>Culture Media</topic><topic>Freund's Adjuvant</topic><topic>Guinea Pigs</topic><topic>Immunization</topic><topic>Lymph Nodes - cytology</topic><topic>Lymphocytes - immunology</topic><topic>Mitogens - analysis</topic><topic>Molecular Conformation</topic><topic>Sucrose</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gately, Celia L</creatorcontrib><creatorcontrib>Gately, Maurice K</creatorcontrib><creatorcontrib>Mayer, Manfred M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gately, Celia L</au><au>Gately, Maurice K</au><au>Mayer, Manfred M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Molecular Dimensions of Mitogenic Factor from Guinea Pig Lymph Node Cells</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1975-01-01</date><risdate>1975</risdate><volume>114</volume><issue>1 Part 1</issue><spage>10</spage><epage>16</epage><pages>10-16</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>The molecular dimensions of a mitogenic factor from guinea pig lymph node cells were investigated by means of gel filtration, sucrose density gradient ultracentrifugation, and CsCi isopycnic ultracentrifugation. The mitogenic factor was produced by stimulation of immune lymph node cells from inbred guinea pigs, strain 2 or 13, with the specific antigen, ovalbumin; and mitogenic activity was assayed by measuring incorporation of tritiated thymidine by target lymph node cells from syngeneic animals not immune to ovalbumin. The D-20,w of the mitogenic factor was estimated to be (9.9 plus and minus 0.2) times 10-7 cm2/sec by gel filtration on Sephadex G-100, and the apparent S20,w was estimated to be 2.4 plus and minus 0.2 S by sucrose density gradient ultracentrifugation. The buoyant density was determined to be 1.324 plus and minus 0.006 g/ml by CsCi isopycnic ultracentrifugation, and from this value the partial specific volume of mitogenic factor was estimated to be 0.71. From these data the molecular weight of the mitogenic factor was calculated to be 20,000, and the frictional ratio was calculated to be 1.2.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>1112974</pmid><doi>10.4049/jimmunol.114.1_Part_1.10</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1975-01, Vol.114 (1 Part 1), p.10-16 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cells, Cultured Centrifugation, Density Gradient Cesium Chromatography, Gel Culture Media Freund's Adjuvant Guinea Pigs Immunization Lymph Nodes - cytology Lymphocytes - immunology Mitogens - analysis Molecular Conformation Sucrose Thymidine - metabolism Tritium Ultracentrifugation |
| title | The Molecular Dimensions of Mitogenic Factor from Guinea Pig Lymph Node Cells |
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