Comparison of mitochondrial components from human and hamster cell lines

Comparison of mitochondrial components from human and hamster cell lines

Mitochondrial DNA (mtDNA) of human (KB) cells has been separated from that of Syrian hamster (BHK) cells on neutral CsCl density gradients, the density difference being 0.008 g/cm 3. For three mitochondrially-located enzymes NAD-malate dehydrogenase, NADP-isocitrate dehydrogenase, and glutamate-oxal...

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Veröffentlicht in:Experimental cell research 1974-01, Vol.89 (2), p.399-401
Hauptverfasser: Strasberg, P.M., Davidson, M.M.L., Wallace, R.B., Freeman, K.B.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Aspartate Aminotransferases - metabolism
Carcinoma
Cell Fractionation
Cell Line
Cell-Free System
Centrifugation, Density Gradient
Cricetinae
DNA, Mitochondrial - analysis
DNA, Neoplasm - analysis
Electrophoresis, Starch Gel
Humans
Hybrid Cells
Isocitrate Dehydrogenase - metabolism
Kidney
Malate Dehydrogenase - metabolism
Mitochondria - analysis
Mitochondria - enzymology
Mouth Neoplasms
Organelle Biogenesis
Space life sciences
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Zusammenfassung:Mitochondrial DNA (mtDNA) of human (KB) cells has been separated from that of Syrian hamster (BHK) cells on neutral CsCl density gradients, the density difference being 0.008 g/cm 3. For three mitochondrially-located enzymes NAD-malate dehydrogenase, NADP-isocitrate dehydrogenase, and glutamate-oxaloacetate transaminase, the human enzyme has been separated from the hamster enzyme by starch gel electrophoresis. These results will facilitate the analysis of human-hamster hybrid cells for the persistence of parental mtDNA and for the presence of parental mitochondrial enzymes.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(74)90805-2