Influence of heparin and protamine on enlargement of the draining lymph node during induction of EAE

Popliteal lymph node enlargement four days after immunization with encephalitogenic guinea pig basic protein in Freund's complete adjuvant (EGPBP-FCA) was less in heparin treated and greater in protamine-treated Lewis rats than in salineinjected controls. These agents were without influence on the node enlargement occasioned by Freund's adjuvant alone or on node size in nonimmunized rats. Decreased cell trapping in the node in heparinized rats and increased trapping in protamine-treated rats immunized with EGPBP-FCA is suggested.