The Application of Fluorescent Antibody Methods to the Study of Platelets
1. Through the use of fluorescent antibody technic, it is possible to detect the antigen-antibody reaction between platelets and heterospecific antiserum, but no antigen-antibody reaction between normal human platelets and sera from patients with idiopathic thrombocytopenic purpura could be demonstr...
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| Veröffentlicht in: | Blood 1960-07, Vol.16 (1), p.958-967 |
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| container_title | Blood |
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| creator | SILBER, ROBERT BENITEZ, ROBERTO EVELAND, WARREN C. ANEROID, JOSEPH H. DUNNE, CHARLES J. |
| description | 1. Through the use of fluorescent antibody technic, it is possible to detect the antigen-antibody reaction between platelets and heterospecific antiserum, but no antigen-antibody reaction between normal human platelets and sera from patients with idiopathic thrombocytopenic purpura could be demonstrated.
2. The method permits the histologic localization of platelets in splenic tissue.
3. Whereas serum proteins can be easily removed from platelets by washing, there is an intimate association with fibrinogen so that this protein is still not removed from the platelet after 10 washings in saline. |
| doi_str_mv | 10.1182/blood.V16.1.958.958 |
| format | Article |
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2. The method permits the histologic localization of platelets in splenic tissue.
3. Whereas serum proteins can be easily removed from platelets by washing, there is an intimate association with fibrinogen so that this protein is still not removed from the platelet after 10 washings in saline.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V16.1.958.958</identifier><identifier>PMID: 14446641</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antigen-Antibody Reactions ; Blood Platelets ; Fluorescent Antibody Technique ; Fluorescent Dyes ; Humans ; Old Medline</subject><ispartof>Blood, 1960-07, Vol.16 (1), p.958-967</ispartof><rights>1960 American Society of Hematology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-6ef45cfc490313e92b4a0f7c2887672f01df3e5671d590b5552b43b441fd7ae33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14446641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SILBER, ROBERT</creatorcontrib><creatorcontrib>BENITEZ, ROBERTO</creatorcontrib><creatorcontrib>EVELAND, WARREN C.</creatorcontrib><creatorcontrib>ANEROID, JOSEPH H.</creatorcontrib><creatorcontrib>DUNNE, CHARLES J.</creatorcontrib><title>The Application of Fluorescent Antibody Methods to the Study of Platelets</title><title>Blood</title><addtitle>Blood</addtitle><description>1. Through the use of fluorescent antibody technic, it is possible to detect the antigen-antibody reaction between platelets and heterospecific antiserum, but no antigen-antibody reaction between normal human platelets and sera from patients with idiopathic thrombocytopenic purpura could be demonstrated.
2. The method permits the histologic localization of platelets in splenic tissue.
3. Whereas serum proteins can be easily removed from platelets by washing, there is an intimate association with fibrinogen so that this protein is still not removed from the platelet after 10 washings in saline.</description><subject>Antigen-Antibody Reactions</subject><subject>Blood Platelets</subject><subject>Fluorescent Antibody Technique</subject><subject>Fluorescent Dyes</subject><subject>Humans</subject><subject>Old Medline</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1960</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlZ_gSB78rZrkk324-ChFKuFioLVa9hNJjSSbuomK_Tfm9qCNw9DYHjemcmD0DXBGSEVvWutcyr7IEVGsppX-zpBY8JplWJM8SkaY4yLlNUlGaEL7z8xJiyn_ByNCGOsKBgZo8VqDcl0u7VGNsG4LnE6mdvB9eAldCGZdsG0Tu2SZwhrp3wSXBJi5C0MsRnhV9sEsBD8JTrTjfVwdXwn6H3-sJo9pcuXx8VsukwlwzSkBWjGpZasxjnJoaYta7AuJa2qsiipxkTpHHhREsVr3HLOI5G3jBGtygbyfIJuD3O3vfsawAexMfFWa5sO3OBFRYuyingE8wMoe-d9D1pse7Np-p0gWOwNil-DIhoURER7-4qpm-P4od2A-ssclUXg_gBA_OS3gV54aaCToEwPMgjlzL8LfgBS2YIw</recordid><startdate>196007</startdate><enddate>196007</enddate><creator>SILBER, ROBERT</creator><creator>BENITEZ, ROBERTO</creator><creator>EVELAND, WARREN C.</creator><creator>ANEROID, JOSEPH H.</creator><creator>DUNNE, CHARLES J.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196007</creationdate><title>The Application of Fluorescent Antibody Methods to the Study of Platelets</title><author>SILBER, ROBERT ; BENITEZ, ROBERTO ; EVELAND, WARREN C. ; ANEROID, JOSEPH H. ; DUNNE, CHARLES J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-6ef45cfc490313e92b4a0f7c2887672f01df3e5671d590b5552b43b441fd7ae33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1960</creationdate><topic>Antigen-Antibody Reactions</topic><topic>Blood Platelets</topic><topic>Fluorescent Antibody Technique</topic><topic>Fluorescent Dyes</topic><topic>Humans</topic><topic>Old Medline</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SILBER, ROBERT</creatorcontrib><creatorcontrib>BENITEZ, ROBERTO</creatorcontrib><creatorcontrib>EVELAND, WARREN C.</creatorcontrib><creatorcontrib>ANEROID, JOSEPH H.</creatorcontrib><creatorcontrib>DUNNE, CHARLES J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SILBER, ROBERT</au><au>BENITEZ, ROBERTO</au><au>EVELAND, WARREN C.</au><au>ANEROID, JOSEPH H.</au><au>DUNNE, CHARLES J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Application of Fluorescent Antibody Methods to the Study of Platelets</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1960-07</date><risdate>1960</risdate><volume>16</volume><issue>1</issue><spage>958</spage><epage>967</epage><pages>958-967</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>1. Through the use of fluorescent antibody technic, it is possible to detect the antigen-antibody reaction between platelets and heterospecific antiserum, but no antigen-antibody reaction between normal human platelets and sera from patients with idiopathic thrombocytopenic purpura could be demonstrated.
2. The method permits the histologic localization of platelets in splenic tissue.
3. Whereas serum proteins can be easily removed from platelets by washing, there is an intimate association with fibrinogen so that this protein is still not removed from the platelet after 10 washings in saline.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>14446641</pmid><doi>10.1182/blood.V16.1.958.958</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 1960-07, Vol.16 (1), p.958-967 |
| issn | 0006-4971 1528-0020 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82678441 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antigen-Antibody Reactions Blood Platelets Fluorescent Antibody Technique Fluorescent Dyes Humans Old Medline |
| title | The Application of Fluorescent Antibody Methods to the Study of Platelets |
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