Factors affecting albumin trailing during electrophoresis
The phenomenon of albumin trailing is examined, and the following observations are made: 1. 1. Albumin trail varies with (a) paper quality and thickness, and (b) varies directly with the voltage applied. 2. 2. Albumin trail is not simply related to (a) the total serum albumin (b) the serum albumin/g...
Gespeichert in:
| Veröffentlicht in: | Clinica chimica acta 1960, Vol.5 (1), p.75-81 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 81 |
|---|---|
| container_issue | 1 |
| container_start_page | 75 |
| container_title | Clinica chimica acta |
| container_volume | 5 |
| creator | Yeoman, W.B. |
| description | The phenomenon of albumin trailing is examined, and the following observations are made:
1.
1. Albumin trail varies with (a) paper quality and thickness, and (b) varies directly with the voltage applied.
2.
2. Albumin trail is not simply related to (a) the total serum albumin (b) the serum albumin/globulin ratio, or (c) the height of the albumin peak of the scandiagram.
3.
3. Albumin trail increases, and the overall migration decreases as the number of strips in the electrophoretic bath is increased.
4.
4. Azocarmine B in the methanolic phase is the most satisfactory dyestuff for the staining of electrophoretic strips. Direct linearity between amount of albumin scanned and scan-area is demonstrated, for the conditions of electrophoresis and of scanning stated.
5.
5. The previous ratio method for estimation of albumin trail from the minimal trough height of the scan-diagram is confirmed. The albumin trail represents between 9% and 14% of the total albumin, if electrophoresis is performed at modest voltages using Whatman No. 1 filter paper. |
| doi_str_mv | 10.1016/0009-8981(60)90092-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82677415</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0009898160900929</els_id><sourcerecordid>82677415</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-b7c220ab3d9553f411b4f56a2f8ddeea80a9219d007c9b97e0ac5c3ee61156433</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMo7rr6D0T2JHqoZpo0bS6CLK4KC170HNJkqpF-rEkr-O9N3aI3T8M7PDPDPIScAr0CCuKaUiqTQhZwIeiljCFN5B6ZQ5GzhHGZ7pP5LzIjRyG8x8ipgEMyA1ZwkYGcE7nWpu98WOqqQtO79nWp63JoXLvsvXb12LCDHwvWEfDd9q3zGFw4JgeVrgOeTHVBXtZ3z6uHZPN0_7i63SSGCdonZW7SlOqSWZllrOIAJa8yodOqsBZRF1TLFKSlNDeylDlSbTLDEAVAJjhjC3K-27v13ceAoVeNCwbrWrfYDUEVqchzDlkE-Q40vgvBY6W23jXafymgalSmRh9q9KEEVT_KlIxjZ9P-oWzQ_g1NjiJwswMwfvnp0KtgHLYGrfPRiLKd-__CN4z1euE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82677415</pqid></control><display><type>article</type><title>Factors affecting albumin trailing during electrophoresis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Yeoman, W.B.</creator><creatorcontrib>Yeoman, W.B.</creatorcontrib><description>The phenomenon of albumin trailing is examined, and the following observations are made:
1.
1. Albumin trail varies with (a) paper quality and thickness, and (b) varies directly with the voltage applied.
2.
2. Albumin trail is not simply related to (a) the total serum albumin (b) the serum albumin/globulin ratio, or (c) the height of the albumin peak of the scandiagram.
3.
3. Albumin trail increases, and the overall migration decreases as the number of strips in the electrophoretic bath is increased.
4.
4. Azocarmine B in the methanolic phase is the most satisfactory dyestuff for the staining of electrophoretic strips. Direct linearity between amount of albumin scanned and scan-area is demonstrated, for the conditions of electrophoresis and of scanning stated.
5.
5. The previous ratio method for estimation of albumin trail from the minimal trough height of the scan-diagram is confirmed. The albumin trail represents between 9% and 14% of the total albumin, if electrophoresis is performed at modest voltages using Whatman No. 1 filter paper.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(60)90092-9</identifier><identifier>PMID: 13846519</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Albumins ; Electrophoresis ; Hematologic Tests ; Old Medline ; Serum Albumin - chemistry</subject><ispartof>Clinica chimica acta, 1960, Vol.5 (1), p.75-81</ispartof><rights>1960</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-b7c220ab3d9553f411b4f56a2f8ddeea80a9219d007c9b97e0ac5c3ee61156433</citedby><cites>FETCH-LOGICAL-c360t-b7c220ab3d9553f411b4f56a2f8ddeea80a9219d007c9b97e0ac5c3ee61156433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898160900929$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13846519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yeoman, W.B.</creatorcontrib><title>Factors affecting albumin trailing during electrophoresis</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>The phenomenon of albumin trailing is examined, and the following observations are made:
1.
1. Albumin trail varies with (a) paper quality and thickness, and (b) varies directly with the voltage applied.
2.
2. Albumin trail is not simply related to (a) the total serum albumin (b) the serum albumin/globulin ratio, or (c) the height of the albumin peak of the scandiagram.
3.
3. Albumin trail increases, and the overall migration decreases as the number of strips in the electrophoretic bath is increased.
4.
4. Azocarmine B in the methanolic phase is the most satisfactory dyestuff for the staining of electrophoretic strips. Direct linearity between amount of albumin scanned and scan-area is demonstrated, for the conditions of electrophoresis and of scanning stated.
5.
5. The previous ratio method for estimation of albumin trail from the minimal trough height of the scan-diagram is confirmed. The albumin trail represents between 9% and 14% of the total albumin, if electrophoresis is performed at modest voltages using Whatman No. 1 filter paper.</description><subject>Albumins</subject><subject>Electrophoresis</subject><subject>Hematologic Tests</subject><subject>Old Medline</subject><subject>Serum Albumin - chemistry</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1960</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6D0T2JHqoZpo0bS6CLK4KC170HNJkqpF-rEkr-O9N3aI3T8M7PDPDPIScAr0CCuKaUiqTQhZwIeiljCFN5B6ZQ5GzhHGZ7pP5LzIjRyG8x8ipgEMyA1ZwkYGcE7nWpu98WOqqQtO79nWp63JoXLvsvXb12LCDHwvWEfDd9q3zGFw4JgeVrgOeTHVBXtZ3z6uHZPN0_7i63SSGCdonZW7SlOqSWZllrOIAJa8yodOqsBZRF1TLFKSlNDeylDlSbTLDEAVAJjhjC3K-27v13ceAoVeNCwbrWrfYDUEVqchzDlkE-Q40vgvBY6W23jXafymgalSmRh9q9KEEVT_KlIxjZ9P-oWzQ_g1NjiJwswMwfvnp0KtgHLYGrfPRiLKd-__CN4z1euE</recordid><startdate>1960</startdate><enddate>1960</enddate><creator>Yeoman, W.B.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1960</creationdate><title>Factors affecting albumin trailing during electrophoresis</title><author>Yeoman, W.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-b7c220ab3d9553f411b4f56a2f8ddeea80a9219d007c9b97e0ac5c3ee61156433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1960</creationdate><topic>Albumins</topic><topic>Electrophoresis</topic><topic>Hematologic Tests</topic><topic>Old Medline</topic><topic>Serum Albumin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yeoman, W.B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yeoman, W.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Factors affecting albumin trailing during electrophoresis</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1960</date><risdate>1960</risdate><volume>5</volume><issue>1</issue><spage>75</spage><epage>81</epage><pages>75-81</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>The phenomenon of albumin trailing is examined, and the following observations are made:
1.
1. Albumin trail varies with (a) paper quality and thickness, and (b) varies directly with the voltage applied.
2.
2. Albumin trail is not simply related to (a) the total serum albumin (b) the serum albumin/globulin ratio, or (c) the height of the albumin peak of the scandiagram.
3.
3. Albumin trail increases, and the overall migration decreases as the number of strips in the electrophoretic bath is increased.
4.
4. Azocarmine B in the methanolic phase is the most satisfactory dyestuff for the staining of electrophoretic strips. Direct linearity between amount of albumin scanned and scan-area is demonstrated, for the conditions of electrophoresis and of scanning stated.
5.
5. The previous ratio method for estimation of albumin trail from the minimal trough height of the scan-diagram is confirmed. The albumin trail represents between 9% and 14% of the total albumin, if electrophoresis is performed at modest voltages using Whatman No. 1 filter paper.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>13846519</pmid><doi>10.1016/0009-8981(60)90092-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1960, Vol.5 (1), p.75-81 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82677415 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Albumins Electrophoresis Hematologic Tests Old Medline Serum Albumin - chemistry |
| title | Factors affecting albumin trailing during electrophoresis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T18%3A12%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Factors%20affecting%20albumin%20trailing%20during%20electrophoresis&rft.jtitle=Clinica%20chimica%20acta&rft.au=Yeoman,%20W.B.&rft.date=1960&rft.volume=5&rft.issue=1&rft.spage=75&rft.epage=81&rft.pages=75-81&rft.issn=0009-8981&rft.eissn=1873-3492&rft_id=info:doi/10.1016/0009-8981(60)90092-9&rft_dat=%3Cproquest_cross%3E82677415%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82677415&rft_id=info:pmid/13846519&rft_els_id=0009898160900929&rfr_iscdi=true |