Biochemical and cytochemical studies of the development of choline acetyltransferase and acetylcholinesterase in organotypic cultures of chick neural tube
Choline acetyltransferase (ChAc) and acetylcholinesterase (AChE) activities have been studied in organotypic cultures of early chick neural tube. During the first week in culture the ChAc activity remains extremely low at which time it can not be demonstrated histochemically in early neurons. It inc...
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| Veröffentlicht in: | Journal of neurobiology 1974, Vol.5 (4), p.305-315 |
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| container_title | Journal of neurobiology |
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| creator | Kim, Seung U. Oh, Tae H. Wenger, Eleanor L. |
| description | Choline acetyltransferase (ChAc) and acetylcholinesterase (AChE) activities have been studied in organotypic cultures of early chick neural tube. During the first week in culture the ChAc activity remains extremely low at which time it can not be demonstrated histochemically in early neurons. It increases rapidly during the second and third weeks in culture, as demonstrated biochemically and histochemically. AChE activity rises rapidly during the first week, and then more gradually for the remainder of the culture period; it can be demonstrated histochemically in early neurons and well differentiated multipolar neurons throughout this period. In our culture system biochemical differentiation (ChAc and AChE activity) occurs concomitantly with morphological differentiation. |
| doi_str_mv | 10.1002/neu.480050403 |
| format | Article |
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During the first week in culture the ChAc activity remains extremely low at which time it can not be demonstrated histochemically in early neurons. It increases rapidly during the second and third weeks in culture, as demonstrated biochemically and histochemically. AChE activity rises rapidly during the first week, and then more gradually for the remainder of the culture period; it can be demonstrated histochemically in early neurons and well differentiated multipolar neurons throughout this period. In our culture system biochemical differentiation (ChAc and AChE activity) occurs concomitantly with morphological differentiation.</description><identifier>ISSN: 0022-3034</identifier><identifier>EISSN: 1097-4695</identifier><identifier>DOI: 10.1002/neu.480050403</identifier><identifier>PMID: 4475689</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Acetylcholinesterase - metabolism ; Acetyltransferases - metabolism ; Age Factors ; Animals ; Cell Differentiation ; Chick Embryo ; Ganglia - cytology ; Histocytochemistry ; Nerve Tissue Proteins - metabolism ; Nervous System - embryology ; Nervous System - enzymology ; Neurons - enzymology ; Organ Culture Techniques ; Organ Specificity</subject><ispartof>Journal of neurobiology, 1974, Vol.5 (4), p.305-315</ispartof><rights>Copyright © 1974 John Wiley & Sons, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3363-cf2ccbe3407fcb0777e1bf51a4e1209fb64eaf9844b09bf62725a6ee40290d433</citedby><cites>FETCH-LOGICAL-c3363-cf2ccbe3407fcb0777e1bf51a4e1209fb64eaf9844b09bf62725a6ee40290d433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fneu.480050403$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fneu.480050403$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4009,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4475689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Seung U.</creatorcontrib><creatorcontrib>Oh, Tae H.</creatorcontrib><creatorcontrib>Wenger, Eleanor L.</creatorcontrib><title>Biochemical and cytochemical studies of the development of choline acetyltransferase and acetylcholinesterase in organotypic cultures of chick neural tube</title><title>Journal of neurobiology</title><addtitle>J Neurobiol</addtitle><description>Choline acetyltransferase (ChAc) and acetylcholinesterase (AChE) activities have been studied in organotypic cultures of early chick neural tube. During the first week in culture the ChAc activity remains extremely low at which time it can not be demonstrated histochemically in early neurons. It increases rapidly during the second and third weeks in culture, as demonstrated biochemically and histochemically. AChE activity rises rapidly during the first week, and then more gradually for the remainder of the culture period; it can be demonstrated histochemically in early neurons and well differentiated multipolar neurons throughout this period. In our culture system biochemical differentiation (ChAc and AChE activity) occurs concomitantly with morphological differentiation.</description><subject>Acetylcholinesterase - metabolism</subject><subject>Acetyltransferases - metabolism</subject><subject>Age Factors</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Chick Embryo</subject><subject>Ganglia - cytology</subject><subject>Histocytochemistry</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Nervous System - embryology</subject><subject>Nervous System - enzymology</subject><subject>Neurons - enzymology</subject><subject>Organ Culture Techniques</subject><subject>Organ Specificity</subject><issn>0022-3034</issn><issn>1097-4695</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtP3DAUha2qiA6PJctKXnWX4cZ2nPGyRbQgobJh1pHjXHdMnXhqO6D8lf5aQjOCXVdXOufouy9CLkpYlwDscsBxLTYAFQjgH8iqBFUXQqrqI1nNPis4cPGJnKT0CABKVeyYHAtRV3KjVuTvNxfMDntntKd66KiZ8ruQ8tg5TDRYmndIO3xCH_Y9DvlVMrvg3YBUG8yTz1EPyWLUCf-BFvWQSXkx3EBD_KWHkKe9M9SMPo9xaWB2zvym8zZxbpzHFs_IkdU-4fmhnpLt9-uHq5vi7v7H7dXXu8JwLnlhLDOmRS6gtqaFuq6xbG1VaoElA2VbKVBbtRGiBdVayWpWaYkogCnoBOen5MvC3cfwZ5xHbXqXDHqvBwxjajZMSl5Wcg4WS9DEkFJE2-yj63WcmhKa11808_TN2y_m_OcDeGx77N7Sh-PPfr34z87j9H9Y8_N6-05-AXe8mfE</recordid><startdate>1974</startdate><enddate>1974</enddate><creator>Kim, Seung U.</creator><creator>Oh, Tae H.</creator><creator>Wenger, Eleanor L.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1974</creationdate><title>Biochemical and cytochemical studies of the development of choline acetyltransferase and acetylcholinesterase in organotypic cultures of chick neural tube</title><author>Kim, Seung U. ; Oh, Tae H. ; Wenger, Eleanor L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3363-cf2ccbe3407fcb0777e1bf51a4e1209fb64eaf9844b09bf62725a6ee40290d433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Acetylcholinesterase - metabolism</topic><topic>Acetyltransferases - metabolism</topic><topic>Age Factors</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Chick Embryo</topic><topic>Ganglia - cytology</topic><topic>Histocytochemistry</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Nervous System - embryology</topic><topic>Nervous System - enzymology</topic><topic>Neurons - enzymology</topic><topic>Organ Culture Techniques</topic><topic>Organ Specificity</topic><toplevel>online_resources</toplevel><creatorcontrib>Kim, Seung U.</creatorcontrib><creatorcontrib>Oh, Tae H.</creatorcontrib><creatorcontrib>Wenger, Eleanor L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Seung U.</au><au>Oh, Tae H.</au><au>Wenger, Eleanor L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical and cytochemical studies of the development of choline acetyltransferase and acetylcholinesterase in organotypic cultures of chick neural tube</atitle><jtitle>Journal of neurobiology</jtitle><addtitle>J Neurobiol</addtitle><date>1974</date><risdate>1974</risdate><volume>5</volume><issue>4</issue><spage>305</spage><epage>315</epage><pages>305-315</pages><issn>0022-3034</issn><eissn>1097-4695</eissn><abstract>Choline acetyltransferase (ChAc) and acetylcholinesterase (AChE) activities have been studied in organotypic cultures of early chick neural tube. During the first week in culture the ChAc activity remains extremely low at which time it can not be demonstrated histochemically in early neurons. It increases rapidly during the second and third weeks in culture, as demonstrated biochemically and histochemically. AChE activity rises rapidly during the first week, and then more gradually for the remainder of the culture period; it can be demonstrated histochemically in early neurons and well differentiated multipolar neurons throughout this period. In our culture system biochemical differentiation (ChAc and AChE activity) occurs concomitantly with morphological differentiation.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>4475689</pmid><doi>10.1002/neu.480050403</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0022-3034 |
| ispartof | Journal of neurobiology, 1974, Vol.5 (4), p.305-315 |
| issn | 0022-3034 1097-4695 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Acetylcholinesterase - metabolism Acetyltransferases - metabolism Age Factors Animals Cell Differentiation Chick Embryo Ganglia - cytology Histocytochemistry Nerve Tissue Proteins - metabolism Nervous System - embryology Nervous System - enzymology Neurons - enzymology Organ Culture Techniques Organ Specificity |
| title | Biochemical and cytochemical studies of the development of choline acetyltransferase and acetylcholinesterase in organotypic cultures of chick neural tube |
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