Epithelial Outgrowth from Suspension Cultures of Human Prostatic Tissue
The primary objective of this study was to obtain pure cultures of prostatic epithelium. Encapsulation by epithelial cells and hypocellularity in stroma occurred when explants of prostatic tissue were maintained in suspension cultures. Twenty per cent fetal bovine serum incorporated into the medium...
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| Veröffentlicht in: | In Vitro 1974-09, Vol.10 (3/4), p.196-205 |
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| container_title | In Vitro |
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| creator | Webber, Mukta M. Oliver G. Stonington Patricia A. Poché |
| description | The primary objective of this study was to obtain pure cultures of prostatic epithelium. Encapsulation by epithelial cells and hypocellularity in stroma occurred when explants of prostatic tissue were maintained in suspension cultures. Twenty per cent fetal bovine serum incorporated into the medium provided optimal conditions for encapsulation and preservation of epithelial cell viability and architecture. Horse serum at the same concentration was less effective. When encapsulated explants were allowed to attach to the substrate, 10 and 20% horse serum favored growth of epithelial cells while fetal bovine serum also stimulated fibroblastic growth. Mechanisms for the induction of hypocellularity, encapsulation, squamous metaplasia and central necrosis in explants were studied. Relationship between the type and concentration of serum and the nature and extent of outgrowth are discussed. |
| doi_str_mv | 10.1007/BF02615233 |
| format | Article |
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Relationship between the type and concentration of serum and the nature and extent of outgrowth are discussed.</description><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02615233</identifier><identifier>PMID: 4476724</identifier><language>eng</language><publisher>United States: Tissue Culture Association, Inc</publisher><subject>3T3 cells ; Animals ; Blood ; Cattle ; Cell culture techniques ; Cell Division ; Cell growth ; Cells, Cultured ; Culture Media ; Cultured cells ; Encapsulation ; Epithelial Cells ; Epithelium ; Epithelium - ultrastructure ; Humans ; Male ; Metaplasia ; Methods ; Mitosis ; Prostate ; Prostate - cytology ; Prostate - ultrastructure ; Time Factors ; Ungulates</subject><ispartof>In Vitro, 1974-09, Vol.10 (3/4), p.196-205</ispartof><rights>Copyright 1975 Tissue Culture Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-ddf594ae84276a834644d4fea7d9aefea443d734544c46476d9d3fa79f7f011f3</citedby><cites>FETCH-LOGICAL-c303t-ddf594ae84276a834644d4fea7d9aefea443d734544c46476d9d3fa79f7f011f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4291802$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4291802$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4476724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Webber, Mukta M.</creatorcontrib><creatorcontrib>Oliver G. Stonington</creatorcontrib><creatorcontrib>Patricia A. Poché</creatorcontrib><title>Epithelial Outgrowth from Suspension Cultures of Human Prostatic Tissue</title><title>In Vitro</title><addtitle>In Vitro</addtitle><description>The primary objective of this study was to obtain pure cultures of prostatic epithelium. Encapsulation by epithelial cells and hypocellularity in stroma occurred when explants of prostatic tissue were maintained in suspension cultures. Twenty per cent fetal bovine serum incorporated into the medium provided optimal conditions for encapsulation and preservation of epithelial cell viability and architecture. Horse serum at the same concentration was less effective. When encapsulated explants were allowed to attach to the substrate, 10 and 20% horse serum favored growth of epithelial cells while fetal bovine serum also stimulated fibroblastic growth. Mechanisms for the induction of hypocellularity, encapsulation, squamous metaplasia and central necrosis in explants were studied. Relationship between the type and concentration of serum and the nature and extent of outgrowth are discussed.</description><subject>3T3 cells</subject><subject>Animals</subject><subject>Blood</subject><subject>Cattle</subject><subject>Cell culture techniques</subject><subject>Cell Division</subject><subject>Cell growth</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Encapsulation</subject><subject>Epithelial Cells</subject><subject>Epithelium</subject><subject>Epithelium - ultrastructure</subject><subject>Humans</subject><subject>Male</subject><subject>Metaplasia</subject><subject>Methods</subject><subject>Mitosis</subject><subject>Prostate</subject><subject>Prostate - cytology</subject><subject>Prostate - ultrastructure</subject><subject>Time Factors</subject><subject>Ungulates</subject><issn>0073-5655</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLw0AAhBdRaq1ePCvsyYMQ3fcmRy19CIUK1nNYs7s2JcnGfSD-eyMt9jSH72MYBoBrjB4wQvLxeY6IwJxQegLGmEmeEZEXp2A8QJpxwfk5uAhhhxBFguARGDEmhSRsDBazvo5b09SqgesUP737jltovWvhWwq96ULtOjhNTUzeBOgsXKZWdfDVuxBVrCu4qUNI5hKcWdUEc3XICXifzzbTZbZaL16mT6usoojGTGvLC6ZMzogUKqdMMKaZNUrqQpkhGaNaUsYZqwYmhS40tUoWVlqEsaUTcLfv7b37SibEsq1DZZpGdcalUOZECIQ5GsT7vVgNS4M3tux93Sr_U2JU_r1WHl8b5NtDa_pojf5XDzcN_GbPdyE6f8SkwDki9BfpHHCB</recordid><startdate>197409</startdate><enddate>197409</enddate><creator>Webber, Mukta M.</creator><creator>Oliver G. 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Stonington</creatorcontrib><creatorcontrib>Patricia A. Poché</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Webber, Mukta M.</au><au>Oliver G. Stonington</au><au>Patricia A. 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| ispartof | In Vitro, 1974-09, Vol.10 (3/4), p.196-205 |
| issn | 0073-5655 1475-2689 |
| language | eng |
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| source | MEDLINE; Jstor Complete Legacy; SpringerLink Journals - AutoHoldings |
| subjects | 3T3 cells Animals Blood Cattle Cell culture techniques Cell Division Cell growth Cells, Cultured Culture Media Cultured cells Encapsulation Epithelial Cells Epithelium Epithelium - ultrastructure Humans Male Metaplasia Methods Mitosis Prostate Prostate - cytology Prostate - ultrastructure Time Factors Ungulates |
| title | Epithelial Outgrowth from Suspension Cultures of Human Prostatic Tissue |
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