REPLICATION OF NEUROTROPIC INFLUENZA VIRUS IN ORGANOTYPIC CULTURES OF EMBRYONIC MOUSE HYPOTHALAMUS
Neurotropic influenza virus (WS-N strain) causes cytolytic changes in organotypic neural cultures derived from embryonic mouse hypothalamus. Initially, nerve cells develop enlarged, refractile nucleoli. In later stages, both nerve and glial cells undergo necrotic changes. These alterations are accom...
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| Veröffentlicht in: | Journal of neuropathology and experimental neurology 1974-10, Vol.33 (5), p.571-581 |
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| container_end_page | 581 |
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| container_issue | 5 |
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| container_title | Journal of neuropathology and experimental neurology |
| container_volume | 33 |
| creator | GAMBOA, EUGENIA T HARTER, DONALD H WOLF, ABNER BENITEZ, HELENA H TAKAHATA, NAOHIKO DUFFY, PHILIP E HSU, KONRAD C |
| description | Neurotropic influenza virus (WS-N strain) causes cytolytic changes in organotypic neural cultures derived from embryonic mouse hypothalamus. Initially, nerve cells develop enlarged, refractile nucleoli. In later stages, both nerve and glial cells undergo necrotic changes. These alterations are accompanied by synthesis of progeny virus and formation of intracellular viral antigen. Electron microscopic observations of infected cultures reveal virus particles with the morphology and dimensions of influenza virions. Organotypic neural cultures may prove useful in studies of influenza virus neurotropism. |
| doi_str_mv | 10.1097/00005072-197410000-00001 |
| format | Article |
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Initially, nerve cells develop enlarged, refractile nucleoli. In later stages, both nerve and glial cells undergo necrotic changes. These alterations are accompanied by synthesis of progeny virus and formation of intracellular viral antigen. Electron microscopic observations of infected cultures reveal virus particles with the morphology and dimensions of influenza virions. 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Initially, nerve cells develop enlarged, refractile nucleoli. In later stages, both nerve and glial cells undergo necrotic changes. These alterations are accompanied by synthesis of progeny virus and formation of intracellular viral antigen. Electron microscopic observations of infected cultures reveal virus particles with the morphology and dimensions of influenza virions. Organotypic neural cultures may prove useful in studies of influenza virus neurotropism.</description><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Cell Nucleus - microbiology</subject><subject>Culture Techniques</subject><subject>Cytopathogenic Effect, Viral</subject><subject>Cytoplasm - microbiology</subject><subject>Fluorescent Antibody Technique</subject><subject>Hypothalamus - embryology</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Organ Specificity</subject><subject>Orthomyxoviridae - growth & development</subject><subject>Orthomyxoviridae - ultrastructure</subject><subject>Virus Cultivation</subject><subject>Virus Replication</subject><issn>0022-3069</issn><issn>1554-6578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UU2P2jAQtVatKEv3J1TKqbe0Hscf8TFFYYkUYhSSldiLZcARtKHsxiDUf19nYbntHGY0896bkd4gFAD-AViKn9gHw4KEIAWFvgv7BHdoCIzRkDMRf0JDjAkJI8zlF3Tv3G_PkFjSARpQDgSADtGqTOd5Nk6qTBWBmgRFWpeqKtU8GwdZMcnrtHhOgqesrBe-D1T5mBSqWvbwuM6rukwXvSyd_SqXqvDTmaoXaTBdzlU1TfJkVi--os-NaZ19uNYRqidpNZ6GuXr0l_NwHTEGoYW1kUxsIsGZ5Q0mjAtjOKGCSBLH3MSYxkLACriQPhuJG8w2oqG4gQbbaIS-X_a-dIfXk3VHvd-5tW1b89ceTk7HhENEOPbE-EJcdwfnOtvol263N90_DVj39up3e_XN3rcReOm3643Tam83N-HVT4_TC34-tEfbuT_t6Ww7vbWmPW71R1-L_gOzWHse</recordid><startdate>197410</startdate><enddate>197410</enddate><creator>GAMBOA, EUGENIA T</creator><creator>HARTER, DONALD H</creator><creator>WOLF, ABNER</creator><creator>BENITEZ, HELENA H</creator><creator>TAKAHATA, NAOHIKO</creator><creator>DUFFY, PHILIP E</creator><creator>HSU, KONRAD C</creator><general>American Association of Neuropathologists, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197410</creationdate><title>REPLICATION OF NEUROTROPIC INFLUENZA VIRUS IN ORGANOTYPIC CULTURES OF EMBRYONIC MOUSE HYPOTHALAMUS</title><author>GAMBOA, EUGENIA T ; HARTER, DONALD H ; WOLF, ABNER ; BENITEZ, HELENA H ; TAKAHATA, NAOHIKO ; DUFFY, PHILIP E ; HSU, KONRAD C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3551-e1ca957d3765e6f02567aa6247292886a8048771b16791b1a90f05d7f40f1f0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Animals</topic><topic>Antigens, Viral - analysis</topic><topic>Cell Nucleus - microbiology</topic><topic>Culture Techniques</topic><topic>Cytopathogenic Effect, Viral</topic><topic>Cytoplasm - microbiology</topic><topic>Fluorescent Antibody Technique</topic><topic>Hypothalamus - embryology</topic><topic>Mice</topic><topic>Microscopy, Electron</topic><topic>Organ Specificity</topic><topic>Orthomyxoviridae - growth & development</topic><topic>Orthomyxoviridae - ultrastructure</topic><topic>Virus Cultivation</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GAMBOA, EUGENIA T</creatorcontrib><creatorcontrib>HARTER, DONALD H</creatorcontrib><creatorcontrib>WOLF, ABNER</creatorcontrib><creatorcontrib>BENITEZ, HELENA H</creatorcontrib><creatorcontrib>TAKAHATA, NAOHIKO</creatorcontrib><creatorcontrib>DUFFY, PHILIP E</creatorcontrib><creatorcontrib>HSU, KONRAD C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuropathology and experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GAMBOA, EUGENIA T</au><au>HARTER, DONALD H</au><au>WOLF, ABNER</au><au>BENITEZ, HELENA H</au><au>TAKAHATA, NAOHIKO</au><au>DUFFY, PHILIP E</au><au>HSU, KONRAD C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>REPLICATION OF NEUROTROPIC INFLUENZA VIRUS IN ORGANOTYPIC CULTURES OF EMBRYONIC MOUSE HYPOTHALAMUS</atitle><jtitle>Journal of neuropathology and experimental neurology</jtitle><addtitle>J Neuropathol Exp Neurol</addtitle><date>1974-10</date><risdate>1974</risdate><volume>33</volume><issue>5</issue><spage>571</spage><epage>581</epage><pages>571-581</pages><issn>0022-3069</issn><eissn>1554-6578</eissn><abstract>Neurotropic influenza virus (WS-N strain) causes cytolytic changes in organotypic neural cultures derived from embryonic mouse hypothalamus. Initially, nerve cells develop enlarged, refractile nucleoli. In later stages, both nerve and glial cells undergo necrotic changes. These alterations are accompanied by synthesis of progeny virus and formation of intracellular viral antigen. Electron microscopic observations of infected cultures reveal virus particles with the morphology and dimensions of influenza virions. Organotypic neural cultures may prove useful in studies of influenza virus neurotropism.</abstract><cop>England</cop><pub>American Association of Neuropathologists, Inc</pub><pmid>4612114</pmid><doi>10.1097/00005072-197410000-00001</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3069 |
| ispartof | Journal of neuropathology and experimental neurology, 1974-10, Vol.33 (5), p.571-581 |
| issn | 0022-3069 1554-6578 |
| language | eng |
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| source | MEDLINE; Oxford Journals A-Z Collection; Journals@Ovid Complete |
| subjects | Animals Antigens, Viral - analysis Cell Nucleus - microbiology Culture Techniques Cytopathogenic Effect, Viral Cytoplasm - microbiology Fluorescent Antibody Technique Hypothalamus - embryology Mice Microscopy, Electron Organ Specificity Orthomyxoviridae - growth & development Orthomyxoviridae - ultrastructure Virus Cultivation Virus Replication |
| title | REPLICATION OF NEUROTROPIC INFLUENZA VIRUS IN ORGANOTYPIC CULTURES OF EMBRYONIC MOUSE HYPOTHALAMUS |
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