Attempts to Extract Infective Nucleic Acid of Rous Sarcoma Virus.
Summary Sodium deoxycholate and hot and cold phenol were used to extract nucleic acid from Rous sarcoma. The hot phenol technic in some experiments yielded a RNAse-sensitive product that caused non-transmissible focal lesions in chick fibroblast tissue cultures, but no extracts initiated a transmiss...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1960-08, Vol.104 (4), p.670-673 |
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| container_end_page | 673 |
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| container_issue | 4 |
| container_start_page | 670 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 104 |
| creator | Weil, Roger Bendich, Aaron Southam, Chester M. |
| description | Summary
Sodium deoxycholate and hot and cold phenol were used to extract nucleic acid from Rous sarcoma. The hot phenol technic in some experiments yielded a RNAse-sensitive product that caused non-transmissible focal lesions in chick fibroblast tissue cultures, but no extracts initiated a transmissible infection in tissue cultures, chicks or embry-onated eggs. The same hot and cold phenol technics did yield infective nucleic acid from West Nile. Mengo, and Semliki Forest virus preparations. Rous sarcoma virus was rapidly inactivated by deoxycholate. |
| doi_str_mv | 10.3181/00379727-104-25947 |
| format | Article |
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Sodium deoxycholate and hot and cold phenol were used to extract nucleic acid from Rous sarcoma. The hot phenol technic in some experiments yielded a RNAse-sensitive product that caused non-transmissible focal lesions in chick fibroblast tissue cultures, but no extracts initiated a transmissible infection in tissue cultures, chicks or embry-onated eggs. The same hot and cold phenol technics did yield infective nucleic acid from West Nile. Mengo, and Semliki Forest virus preparations. Rous sarcoma virus was rapidly inactivated by deoxycholate.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-104-25947</identifier><identifier>PMID: 13783840</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Neoplasms ; Nucleic Acids - virology ; Old Medline ; Rous sarcoma virus ; Sarcoma - virology</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1960-08, Vol.104 (4), p.670-673</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-e5f00388d4b37847dc1ee6a62d5416c9ac478cd229a83ddff60a402500eee6783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13783840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weil, Roger</creatorcontrib><creatorcontrib>Bendich, Aaron</creatorcontrib><creatorcontrib>Southam, Chester M.</creatorcontrib><title>Attempts to Extract Infective Nucleic Acid of Rous Sarcoma Virus.</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
Sodium deoxycholate and hot and cold phenol were used to extract nucleic acid from Rous sarcoma. The hot phenol technic in some experiments yielded a RNAse-sensitive product that caused non-transmissible focal lesions in chick fibroblast tissue cultures, but no extracts initiated a transmissible infection in tissue cultures, chicks or embry-onated eggs. The same hot and cold phenol technics did yield infective nucleic acid from West Nile. Mengo, and Semliki Forest virus preparations. Rous sarcoma virus was rapidly inactivated by deoxycholate.</description><subject>Neoplasms</subject><subject>Nucleic Acids - virology</subject><subject>Old Medline</subject><subject>Rous sarcoma virus</subject><subject>Sarcoma - virology</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1960</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC0EoqXwBzggn7il9Suxc6yqApUqkHhdLdfeoFRJU2wHwb_HpUXcOK20-mZ2dhC6pGTMqaITQrgsJZMZJSJjeSnkERrSnOcZL8ryGA13QLYjBugshDUhNJesOEUDyqXiSpAhmk5jhHYbA44dnn9Gb2zEi00FNtYfgO9720Bt8dTWDncVfuz6gJ-Mt11r8Gvt-zA-RyeVaQJcHOYIvdzMn2d32fLhdjGbLjPLBY0Z5FWKo5QTq3RcSGcpQGEK5nJBC1saK6SyjrHSKO5cVRXECMJyQiBxKe4IXe99t7577yFE3dbBQtOYDaRUWrGCEqrKBLI9aH0XgodKb33dGv-lKdG74vRvcWkh9E9xSXR1cO9XLbg_yaGpBEz2QDBvoNdd7zfp2_8svwEuynUD</recordid><startdate>196008</startdate><enddate>196008</enddate><creator>Weil, Roger</creator><creator>Bendich, Aaron</creator><creator>Southam, Chester M.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196008</creationdate><title>Attempts to Extract Infective Nucleic Acid of Rous Sarcoma Virus.</title><author>Weil, Roger ; Bendich, Aaron ; Southam, Chester M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-e5f00388d4b37847dc1ee6a62d5416c9ac478cd229a83ddff60a402500eee6783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1960</creationdate><topic>Neoplasms</topic><topic>Nucleic Acids - virology</topic><topic>Old Medline</topic><topic>Rous sarcoma virus</topic><topic>Sarcoma - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weil, Roger</creatorcontrib><creatorcontrib>Bendich, Aaron</creatorcontrib><creatorcontrib>Southam, Chester M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weil, Roger</au><au>Bendich, Aaron</au><au>Southam, Chester M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Attempts to Extract Infective Nucleic Acid of Rous Sarcoma Virus.</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1960-08</date><risdate>1960</risdate><volume>104</volume><issue>4</issue><spage>670</spage><epage>673</epage><pages>670-673</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
Sodium deoxycholate and hot and cold phenol were used to extract nucleic acid from Rous sarcoma. The hot phenol technic in some experiments yielded a RNAse-sensitive product that caused non-transmissible focal lesions in chick fibroblast tissue cultures, but no extracts initiated a transmissible infection in tissue cultures, chicks or embry-onated eggs. The same hot and cold phenol technics did yield infective nucleic acid from West Nile. Mengo, and Semliki Forest virus preparations. Rous sarcoma virus was rapidly inactivated by deoxycholate.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>13783840</pmid><doi>10.3181/00379727-104-25947</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1960-08, Vol.104 (4), p.670-673 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82610189 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Neoplasms Nucleic Acids - virology Old Medline Rous sarcoma virus Sarcoma - virology |
| title | Attempts to Extract Infective Nucleic Acid of Rous Sarcoma Virus. |
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