Isolation and characterization of norleucine-resistant baby hamster kidney cells
Variants resistant to low and high levels of the methionine analogue norleucine were isolated in baby hamster kidney cells and in two clonal sublines, B1 and TG2. Clones resistant to high levels of norleucine were observed only after chemical mutagenesis, whereas clones capable of growing in low con...
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| Veröffentlicht in: | Experimental cell research 1974-11, Vol.89 (1), p.154-160 |
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| creator | Kamely, Daphne Littlefield, J.W. |
| description | Variants resistant to low and high levels of the methionine analogue norleucine were isolated in baby hamster kidney cells and in two clonal sublines, B1 and TG2. Clones resistant to high levels of norleucine were observed only after chemical mutagenesis, whereas clones capable of growing in low concentrations of norleucine occurred with equal frequency spontaneously and after mutagenesis. The variants were characterized with respect to uptake of
14C-norleucine and
14C-methionine. Five clones were found to be deficient in
14C-norleucine uptake, and of these, four showed reduced
14C-methionine uptake. The variants were tested also for increased activity of N
5-methyl-tetrahydrofolate: homocysteine methyltransferase, the enzyme which catalyses the terminal reaction in methionine biosynthesis. In four clones, higher levels of the methyltransferase were present than in the wild-type cells, suggesting overproduction or stabilization of this enzyme. |
| doi_str_mv | 10.1016/0014-4827(74)90198-0 |
| format | Article |
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14C-norleucine and
14C-methionine. Five clones were found to be deficient in
14C-norleucine uptake, and of these, four showed reduced
14C-methionine uptake. The variants were tested also for increased activity of N
5-methyl-tetrahydrofolate: homocysteine methyltransferase, the enzyme which catalyses the terminal reaction in methionine biosynthesis. In four clones, higher levels of the methyltransferase were present than in the wild-type cells, suggesting overproduction or stabilization of this enzyme.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(74)90198-0</identifier><identifier>PMID: 4373259</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aminocaproates - metabolism ; Animals ; Biological Transport ; Bromodeoxyuridine - pharmacology ; Carbon Radioisotopes ; Cell Line - metabolism ; Cell Survival - drug effects ; Clone Cells ; Cricetinae ; Drug Resistance ; Homocysteine ; Kidney ; Mesylates - pharmacology ; Methionine - metabolism ; Methyltransferases - metabolism ; Nitrosoguanidines - pharmacology ; Norleucine - metabolism ; Norleucine - pharmacology ; Temperature ; Tetrahydrofolates ; Time Factors</subject><ispartof>Experimental cell research, 1974-11, Vol.89 (1), p.154-160</ispartof><rights>1974</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-b57abe5af0bdaa8eee46a1acc66823b3d32a8cbc5be89119fe4968c4eef2bba03</citedby><cites>FETCH-LOGICAL-c357t-b57abe5af0bdaa8eee46a1acc66823b3d32a8cbc5be89119fe4968c4eef2bba03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(74)90198-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4373259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamely, Daphne</creatorcontrib><creatorcontrib>Littlefield, J.W.</creatorcontrib><title>Isolation and characterization of norleucine-resistant baby hamster kidney cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Variants resistant to low and high levels of the methionine analogue norleucine were isolated in baby hamster kidney cells and in two clonal sublines, B1 and TG2. Clones resistant to high levels of norleucine were observed only after chemical mutagenesis, whereas clones capable of growing in low concentrations of norleucine occurred with equal frequency spontaneously and after mutagenesis. The variants were characterized with respect to uptake of
14C-norleucine and
14C-methionine. Five clones were found to be deficient in
14C-norleucine uptake, and of these, four showed reduced
14C-methionine uptake. The variants were tested also for increased activity of N
5-methyl-tetrahydrofolate: homocysteine methyltransferase, the enzyme which catalyses the terminal reaction in methionine biosynthesis. In four clones, higher levels of the methyltransferase were present than in the wild-type cells, suggesting overproduction or stabilization of this enzyme.</description><subject>Aminocaproates - metabolism</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Bromodeoxyuridine - pharmacology</subject><subject>Carbon Radioisotopes</subject><subject>Cell Line - metabolism</subject><subject>Cell Survival - drug effects</subject><subject>Clone Cells</subject><subject>Cricetinae</subject><subject>Drug Resistance</subject><subject>Homocysteine</subject><subject>Kidney</subject><subject>Mesylates - pharmacology</subject><subject>Methionine - metabolism</subject><subject>Methyltransferases - metabolism</subject><subject>Nitrosoguanidines - pharmacology</subject><subject>Norleucine - metabolism</subject><subject>Norleucine - pharmacology</subject><subject>Temperature</subject><subject>Tetrahydrofolates</subject><subject>Time Factors</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6DRR6Ej1UkzRt04sgi38WFvSg5zBJp2y0TdekFdZP79Yue_Q0MPPem5kfIeeM3jDKsltKmYiF5PlVLq4LygoZ0wMyZbSgMRecH5LpXnJMTkL4oJRKybIJmYgkT3haTMnrIrQ1dLZ1EbgyMivwYDr09mdstlXkWl9jb6zD2GOwoQPXRRr0JlpBE7ba6NOWDjeRwboOp-Sogjrg2a7OyPvjw9v8OV6-PC3m98vYJGnexTrNQWMKFdUlgEREkQEDY7JM8kQnZcJBGm1SjbJgrKhQFJk0ArHiWgNNZuRyzF379qvH0KnGhuECcNj2QUmeSpplg1CMQuPbEDxWau1tA36jGFUDSDVQUgMllQv1B1INtotdfq8bLPemHbnt_G6c4_bJb4teBWPRGSytR9OpsrX_L_gF1aOErg</recordid><startdate>197411</startdate><enddate>197411</enddate><creator>Kamely, Daphne</creator><creator>Littlefield, J.W.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197411</creationdate><title>Isolation and characterization of norleucine-resistant baby hamster kidney cells</title><author>Kamely, Daphne ; Littlefield, J.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-b57abe5af0bdaa8eee46a1acc66823b3d32a8cbc5be89119fe4968c4eef2bba03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Aminocaproates - metabolism</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Bromodeoxyuridine - pharmacology</topic><topic>Carbon Radioisotopes</topic><topic>Cell Line - metabolism</topic><topic>Cell Survival - drug effects</topic><topic>Clone Cells</topic><topic>Cricetinae</topic><topic>Drug Resistance</topic><topic>Homocysteine</topic><topic>Kidney</topic><topic>Mesylates - pharmacology</topic><topic>Methionine - metabolism</topic><topic>Methyltransferases - metabolism</topic><topic>Nitrosoguanidines - pharmacology</topic><topic>Norleucine - metabolism</topic><topic>Norleucine - pharmacology</topic><topic>Temperature</topic><topic>Tetrahydrofolates</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kamely, Daphne</creatorcontrib><creatorcontrib>Littlefield, J.W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamely, Daphne</au><au>Littlefield, J.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of norleucine-resistant baby hamster kidney cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1974-11</date><risdate>1974</risdate><volume>89</volume><issue>1</issue><spage>154</spage><epage>160</epage><pages>154-160</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Variants resistant to low and high levels of the methionine analogue norleucine were isolated in baby hamster kidney cells and in two clonal sublines, B1 and TG2. Clones resistant to high levels of norleucine were observed only after chemical mutagenesis, whereas clones capable of growing in low concentrations of norleucine occurred with equal frequency spontaneously and after mutagenesis. The variants were characterized with respect to uptake of
14C-norleucine and
14C-methionine. Five clones were found to be deficient in
14C-norleucine uptake, and of these, four showed reduced
14C-methionine uptake. The variants were tested also for increased activity of N
5-methyl-tetrahydrofolate: homocysteine methyltransferase, the enzyme which catalyses the terminal reaction in methionine biosynthesis. In four clones, higher levels of the methyltransferase were present than in the wild-type cells, suggesting overproduction or stabilization of this enzyme.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4373259</pmid><doi>10.1016/0014-4827(74)90198-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1974-11, Vol.89 (1), p.154-160 |
| issn | 0014-4827 1090-2422 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82580660 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aminocaproates - metabolism Animals Biological Transport Bromodeoxyuridine - pharmacology Carbon Radioisotopes Cell Line - metabolism Cell Survival - drug effects Clone Cells Cricetinae Drug Resistance Homocysteine Kidney Mesylates - pharmacology Methionine - metabolism Methyltransferases - metabolism Nitrosoguanidines - pharmacology Norleucine - metabolism Norleucine - pharmacology Temperature Tetrahydrofolates Time Factors |
| title | Isolation and characterization of norleucine-resistant baby hamster kidney cells |
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