Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate
The site and rate of synthesis, as well as the transport dynamics, of newly synthesized acetylcholinesterase (AChE) has been studied in the septum and hippocampus of the rat. Histochemical and biochemical techniques were employed to study time-dependent changes in AChE activity of the medial septal...
Gespeichert in:
| Veröffentlicht in: | Brain research 1974-12, Vol.81 (3), p.485-496 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 496 |
|---|---|
| container_issue | 3 |
| container_start_page | 485 |
| container_title | Brain research |
| container_volume | 81 |
| creator | Chippendale, Thomas J. Cotman, Carl W. Kozar, Mark D. Linch, Gary S. |
| description | The site and rate of synthesis, as well as the transport dynamics, of newly synthesized acetylcholinesterase (AChE) has been studied in the septum and hippocampus of the rat. Histochemical and biochemical techniques were employed to study time-dependent changes in AChE activity of the medial septal nucleus and hippocampus following systemic administration of the anticholinesterase diisopropyl-fluorophosphate. In both septum and hippocampus, an initially rapid phase of recovery was followed by a slower recovery with similar rate constants for the two regions. Analysis of AChE synthesis in the septo-hippocampal system revealed that recovery of activity in the septum(t
1/2for recovery= 140h) preceded that in the hippocampus(t
1/2for recovery= 400h). The data suggested that the bulk of septal AChE in the hippocampus is transported via the slow component of axoplasmic flow (about 2 mm/day). A fast component may exist, but the experimental arrangement did not permit the clear demonstration of any rapid axoplasmic flow. |
| doi_str_mv | 10.1016/0006-8993(74)90845-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82570108</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006899374908452</els_id><sourcerecordid>82570108</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-5dc4bfa3be7d6ce5d7e900f8a36ecd1477bbd598610d0957f9dc1b2151723a1e3</originalsourceid><addsrcrecordid>eNp9kUGP1SAQx4nRrM_Vb6AJJ6OHKrS0tB5MNhtXTTbxomdCYZpiWqgMfUk_o19Kuu-52YuegJn__IaZPyEvOXvHGW_eM8aaou266o0UbzvWirooH5EDb2VZNKVgj8nhXvKUPEP8mZ9V1bELciG4YIyzA_l95fW0oUMaBqoNpG0yY5icB0wQNQLFzacRdoX2lqaoPS4hJuo8zXEadaKjW5Zg9Lys-IFGMOEIcfsnUJvkji5tfwkIS1rnO_oDENVD1lNtZ-cd5rbJBb8zrXMYlhiWbRqmNeTLGHAZdYLn5MmgJ4QX5_OS_Lj59P36S3H77fPX66vbwlS1TEVtjegHXfUgbWOgthI6xoZWVw0Yy4WUfW_rrm04s6yr5dBZw_uS11yWleZQXZLXJ27-xa81z6VmhwamSXsIK6q2rGVebpuF4iQ0MSBGGNQS3azjpjhTu4dqN0jtBikp1J2Hqsxlr878tZ_B3hedTcv5j6c85CGPDqJC48AbsC4vPykb3P8b_AGtPbQc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82570108</pqid></control><display><type>article</type><title>Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Chippendale, Thomas J. ; Cotman, Carl W. ; Kozar, Mark D. ; Linch, Gary S.</creator><creatorcontrib>Chippendale, Thomas J. ; Cotman, Carl W. ; Kozar, Mark D. ; Linch, Gary S.</creatorcontrib><description>The site and rate of synthesis, as well as the transport dynamics, of newly synthesized acetylcholinesterase (AChE) has been studied in the septum and hippocampus of the rat. Histochemical and biochemical techniques were employed to study time-dependent changes in AChE activity of the medial septal nucleus and hippocampus following systemic administration of the anticholinesterase diisopropyl-fluorophosphate. In both septum and hippocampus, an initially rapid phase of recovery was followed by a slower recovery with similar rate constants for the two regions. Analysis of AChE synthesis in the septo-hippocampal system revealed that recovery of activity in the septum(t
1/2for recovery= 140h) preceded that in the hippocampus(t
1/2for recovery= 400h). The data suggested that the bulk of septal AChE in the hippocampus is transported via the slow component of axoplasmic flow (about 2 mm/day). A fast component may exist, but the experimental arrangement did not permit the clear demonstration of any rapid axoplasmic flow.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(74)90845-2</identifier><identifier>PMID: 4140010</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acetylcholinesterase - biosynthesis ; Acetylcholinesterase - metabolism ; Animals ; Axonal Transport - drug effects ; Biological Transport, Active - drug effects ; Brain Mapping ; Hippocampus - drug effects ; Hippocampus - enzymology ; Histocytochemistry ; Isoflurophate - pharmacology ; Male ; Neural Pathways ; Rats ; Septum Pellucidum - drug effects ; Septum Pellucidum - enzymology ; Time Factors</subject><ispartof>Brain research, 1974-12, Vol.81 (3), p.485-496</ispartof><rights>1974 Elsevier Scientific Publishing Company Amsterdam</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-5dc4bfa3be7d6ce5d7e900f8a36ecd1477bbd598610d0957f9dc1b2151723a1e3</citedby><cites>FETCH-LOGICAL-c357t-5dc4bfa3be7d6ce5d7e900f8a36ecd1477bbd598610d0957f9dc1b2151723a1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-8993(74)90845-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4140010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chippendale, Thomas J.</creatorcontrib><creatorcontrib>Cotman, Carl W.</creatorcontrib><creatorcontrib>Kozar, Mark D.</creatorcontrib><creatorcontrib>Linch, Gary S.</creatorcontrib><title>Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The site and rate of synthesis, as well as the transport dynamics, of newly synthesized acetylcholinesterase (AChE) has been studied in the septum and hippocampus of the rat. Histochemical and biochemical techniques were employed to study time-dependent changes in AChE activity of the medial septal nucleus and hippocampus following systemic administration of the anticholinesterase diisopropyl-fluorophosphate. In both septum and hippocampus, an initially rapid phase of recovery was followed by a slower recovery with similar rate constants for the two regions. Analysis of AChE synthesis in the septo-hippocampal system revealed that recovery of activity in the septum(t
1/2for recovery= 140h) preceded that in the hippocampus(t
1/2for recovery= 400h). The data suggested that the bulk of septal AChE in the hippocampus is transported via the slow component of axoplasmic flow (about 2 mm/day). A fast component may exist, but the experimental arrangement did not permit the clear demonstration of any rapid axoplasmic flow.</description><subject>Acetylcholinesterase - biosynthesis</subject><subject>Acetylcholinesterase - metabolism</subject><subject>Animals</subject><subject>Axonal Transport - drug effects</subject><subject>Biological Transport, Active - drug effects</subject><subject>Brain Mapping</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - enzymology</subject><subject>Histocytochemistry</subject><subject>Isoflurophate - pharmacology</subject><subject>Male</subject><subject>Neural Pathways</subject><subject>Rats</subject><subject>Septum Pellucidum - drug effects</subject><subject>Septum Pellucidum - enzymology</subject><subject>Time Factors</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGP1SAQx4nRrM_Vb6AJJ6OHKrS0tB5MNhtXTTbxomdCYZpiWqgMfUk_o19Kuu-52YuegJn__IaZPyEvOXvHGW_eM8aaou266o0UbzvWirooH5EDb2VZNKVgj8nhXvKUPEP8mZ9V1bELciG4YIyzA_l95fW0oUMaBqoNpG0yY5icB0wQNQLFzacRdoX2lqaoPS4hJuo8zXEadaKjW5Zg9Lys-IFGMOEIcfsnUJvkji5tfwkIS1rnO_oDENVD1lNtZ-cd5rbJBb8zrXMYlhiWbRqmNeTLGHAZdYLn5MmgJ4QX5_OS_Lj59P36S3H77fPX66vbwlS1TEVtjegHXfUgbWOgthI6xoZWVw0Yy4WUfW_rrm04s6yr5dBZw_uS11yWleZQXZLXJ27-xa81z6VmhwamSXsIK6q2rGVebpuF4iQ0MSBGGNQS3azjpjhTu4dqN0jtBikp1J2Hqsxlr878tZ_B3hedTcv5j6c85CGPDqJC48AbsC4vPykb3P8b_AGtPbQc</recordid><startdate>19741213</startdate><enddate>19741213</enddate><creator>Chippendale, Thomas J.</creator><creator>Cotman, Carl W.</creator><creator>Kozar, Mark D.</creator><creator>Linch, Gary S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19741213</creationdate><title>Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate</title><author>Chippendale, Thomas J. ; Cotman, Carl W. ; Kozar, Mark D. ; Linch, Gary S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-5dc4bfa3be7d6ce5d7e900f8a36ecd1477bbd598610d0957f9dc1b2151723a1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Acetylcholinesterase - biosynthesis</topic><topic>Acetylcholinesterase - metabolism</topic><topic>Animals</topic><topic>Axonal Transport - drug effects</topic><topic>Biological Transport, Active - drug effects</topic><topic>Brain Mapping</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - enzymology</topic><topic>Histocytochemistry</topic><topic>Isoflurophate - pharmacology</topic><topic>Male</topic><topic>Neural Pathways</topic><topic>Rats</topic><topic>Septum Pellucidum - drug effects</topic><topic>Septum Pellucidum - enzymology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chippendale, Thomas J.</creatorcontrib><creatorcontrib>Cotman, Carl W.</creatorcontrib><creatorcontrib>Kozar, Mark D.</creatorcontrib><creatorcontrib>Linch, Gary S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chippendale, Thomas J.</au><au>Cotman, Carl W.</au><au>Kozar, Mark D.</au><au>Linch, Gary S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1974-12-13</date><risdate>1974</risdate><volume>81</volume><issue>3</issue><spage>485</spage><epage>496</epage><pages>485-496</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>The site and rate of synthesis, as well as the transport dynamics, of newly synthesized acetylcholinesterase (AChE) has been studied in the septum and hippocampus of the rat. Histochemical and biochemical techniques were employed to study time-dependent changes in AChE activity of the medial septal nucleus and hippocampus following systemic administration of the anticholinesterase diisopropyl-fluorophosphate. In both septum and hippocampus, an initially rapid phase of recovery was followed by a slower recovery with similar rate constants for the two regions. Analysis of AChE synthesis in the septo-hippocampal system revealed that recovery of activity in the septum(t
1/2for recovery= 140h) preceded that in the hippocampus(t
1/2for recovery= 400h). The data suggested that the bulk of septal AChE in the hippocampus is transported via the slow component of axoplasmic flow (about 2 mm/day). A fast component may exist, but the experimental arrangement did not permit the clear demonstration of any rapid axoplasmic flow.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>4140010</pmid><doi>10.1016/0006-8993(74)90845-2</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-8993 |
| ispartof | Brain research, 1974-12, Vol.81 (3), p.485-496 |
| issn | 0006-8993 1872-6240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82570108 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetylcholinesterase - biosynthesis Acetylcholinesterase - metabolism Animals Axonal Transport - drug effects Biological Transport, Active - drug effects Brain Mapping Hippocampus - drug effects Hippocampus - enzymology Histocytochemistry Isoflurophate - pharmacology Male Neural Pathways Rats Septum Pellucidum - drug effects Septum Pellucidum - enzymology Time Factors |
| title | Analysis of acetylcholinesterase synthesis and transport in the rat hippocampus: recovery of acetylcholinesterase activity in the septum and hippocampus after administration of diisopropylfluorophosphate |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T21%3A04%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20acetylcholinesterase%20synthesis%20and%20transport%20in%20the%20rat%20hippocampus:%20recovery%20of%20acetylcholinesterase%20activity%20in%20the%20septum%20and%20hippocampus%20after%20administration%20of%20diisopropylfluorophosphate&rft.jtitle=Brain%20research&rft.au=Chippendale,%20Thomas%20J.&rft.date=1974-12-13&rft.volume=81&rft.issue=3&rft.spage=485&rft.epage=496&rft.pages=485-496&rft.issn=0006-8993&rft.eissn=1872-6240&rft_id=info:doi/10.1016/0006-8993(74)90845-2&rft_dat=%3Cproquest_cross%3E82570108%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82570108&rft_id=info:pmid/4140010&rft_els_id=0006899374908452&rfr_iscdi=true |