A Study of a New Human Tumor Cell Line (Rhabdomyosarcoma)
Summary A rhabdomyosarcoma cell culture (HUS-2) was established from an 80 year old female. The cells underwent a morphologic change (at the 12-14 cell transfer) but the karyotypic pattern remained stable throughout. It was our impression that this morphologic change was not an example of transforma...
Gespeichert in:
| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1974-09, Vol.146 (4), p.1087-1092 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1092 |
|---|---|
| container_issue | 4 |
| container_start_page | 1087 |
| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 146 |
| creator | Chapman, A. L. Bogner, Phil Behbehani, Abbas M. |
| description | Summary
A rhabdomyosarcoma cell culture (HUS-2) was established from an 80 year old female. The cells underwent a morphologic change (at the 12-14 cell transfer) but the karyotypic pattern remained stable throughout. It was our impression that this morphologic change was not an example of transformation but simply an adaptative process of these cells to the culture conditions. The modal number was 58 with a telocentric marker chromosome before and after the morphologic alteration. Treatment with 5-Iododeoxy-uridine and 20-methylcholantrene and DEAE-D caused no karyotypic change. Electron microscopy showed masses of fibrillar material in the multinucleated giant cells and no virus particles were seen in any culture examined. |
| doi_str_mv | 10.3181/00379727-146-38250 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82557189</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.3181_00379727-146-38250</sage_id><sourcerecordid>82557189</sourcerecordid><originalsourceid>FETCH-LOGICAL-c338t-dc27d77eb5e839cf846e46c21ee37c7fa2c2071a67187a7826eb2bc6eb0772733</originalsourceid><addsrcrecordid>eNp9kM1KAzEURoMotVZfQBCyEl3E5mdmklmWUq1QFLSuQyZzR1uaSU06SN_e1FaXbpLFPd_HvQehS0bvBFNsSKmQpeSSsKwgQvGcHqE-y0VORFGWx6i_A8iOOEVnMS4pZbnkRQ_1sizBgvZROcKvm67eYt9gg5_gC087Z1o875wPeAyrFZ4tWsA3Lx-mqr3b-miC9c7cnqOTxqwiXBz-AXq7n8zHUzJ7fngcj2bECqE2pLZc1lJClYMSpW1UVkBWWM4AhLSyMdxyKpkpJFPSSMULqHhl00tlWlyIAbre966D_-wgbrRbRJsWMy34Lup0dp6yZQL5HrTBxxig0euwcCZsNaN650v_-tLJl_7xlUJXh_auclD_RQ6C0ny4n0fzDnrpu9CmY_9r_Ab6RHDM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82557189</pqid></control><display><type>article</type><title>A Study of a New Human Tumor Cell Line (Rhabdomyosarcoma)</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Chapman, A. L. ; Bogner, Phil ; Behbehani, Abbas M.</creator><creatorcontrib>Chapman, A. L. ; Bogner, Phil ; Behbehani, Abbas M.</creatorcontrib><description>Summary
A rhabdomyosarcoma cell culture (HUS-2) was established from an 80 year old female. The cells underwent a morphologic change (at the 12-14 cell transfer) but the karyotypic pattern remained stable throughout. It was our impression that this morphologic change was not an example of transformation but simply an adaptative process of these cells to the culture conditions. The modal number was 58 with a telocentric marker chromosome before and after the morphologic alteration. Treatment with 5-Iododeoxy-uridine and 20-methylcholantrene and DEAE-D caused no karyotypic change. Electron microscopy showed masses of fibrillar material in the multinucleated giant cells and no virus particles were seen in any culture examined.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-146-38250</identifier><identifier>PMID: 4425030</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Cell Line ; Cell Nucleus - ultrastructure ; Cells, Cultured ; Chromosomes - drug effects ; Deoxyuridine - pharmacology ; Dextrans - pharmacology ; Ethylamines - pharmacology ; Humans ; Iodoproteins ; Karyotyping ; Methylcholanthrene - pharmacology ; Microscopy, Electron ; Oncogenic Viruses - isolation & purification ; Rhabdomyosarcoma - microbiology ; Rhabdomyosarcoma - pathology</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1974-09, Vol.146 (4), p.1087-1092</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-dc27d77eb5e839cf846e46c21ee37c7fa2c2071a67187a7826eb2bc6eb0772733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4425030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chapman, A. L.</creatorcontrib><creatorcontrib>Bogner, Phil</creatorcontrib><creatorcontrib>Behbehani, Abbas M.</creatorcontrib><title>A Study of a New Human Tumor Cell Line (Rhabdomyosarcoma)</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
A rhabdomyosarcoma cell culture (HUS-2) was established from an 80 year old female. The cells underwent a morphologic change (at the 12-14 cell transfer) but the karyotypic pattern remained stable throughout. It was our impression that this morphologic change was not an example of transformation but simply an adaptative process of these cells to the culture conditions. The modal number was 58 with a telocentric marker chromosome before and after the morphologic alteration. Treatment with 5-Iododeoxy-uridine and 20-methylcholantrene and DEAE-D caused no karyotypic change. Electron microscopy showed masses of fibrillar material in the multinucleated giant cells and no virus particles were seen in any culture examined.</description><subject>Cell Line</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cells, Cultured</subject><subject>Chromosomes - drug effects</subject><subject>Deoxyuridine - pharmacology</subject><subject>Dextrans - pharmacology</subject><subject>Ethylamines - pharmacology</subject><subject>Humans</subject><subject>Iodoproteins</subject><subject>Karyotyping</subject><subject>Methylcholanthrene - pharmacology</subject><subject>Microscopy, Electron</subject><subject>Oncogenic Viruses - isolation & purification</subject><subject>Rhabdomyosarcoma - microbiology</subject><subject>Rhabdomyosarcoma - pathology</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1KAzEURoMotVZfQBCyEl3E5mdmklmWUq1QFLSuQyZzR1uaSU06SN_e1FaXbpLFPd_HvQehS0bvBFNsSKmQpeSSsKwgQvGcHqE-y0VORFGWx6i_A8iOOEVnMS4pZbnkRQ_1sizBgvZROcKvm67eYt9gg5_gC087Z1o875wPeAyrFZ4tWsA3Lx-mqr3b-miC9c7cnqOTxqwiXBz-AXq7n8zHUzJ7fngcj2bECqE2pLZc1lJClYMSpW1UVkBWWM4AhLSyMdxyKpkpJFPSSMULqHhl00tlWlyIAbre966D_-wgbrRbRJsWMy34Lup0dp6yZQL5HrTBxxig0euwcCZsNaN650v_-tLJl_7xlUJXh_auclD_RQ6C0ny4n0fzDnrpu9CmY_9r_Ab6RHDM</recordid><startdate>197409</startdate><enddate>197409</enddate><creator>Chapman, A. L.</creator><creator>Bogner, Phil</creator><creator>Behbehani, Abbas M.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197409</creationdate><title>A Study of a New Human Tumor Cell Line (Rhabdomyosarcoma)</title><author>Chapman, A. L. ; Bogner, Phil ; Behbehani, Abbas M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-dc27d77eb5e839cf846e46c21ee37c7fa2c2071a67187a7826eb2bc6eb0772733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Cell Line</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cells, Cultured</topic><topic>Chromosomes - drug effects</topic><topic>Deoxyuridine - pharmacology</topic><topic>Dextrans - pharmacology</topic><topic>Ethylamines - pharmacology</topic><topic>Humans</topic><topic>Iodoproteins</topic><topic>Karyotyping</topic><topic>Methylcholanthrene - pharmacology</topic><topic>Microscopy, Electron</topic><topic>Oncogenic Viruses - isolation & purification</topic><topic>Rhabdomyosarcoma - microbiology</topic><topic>Rhabdomyosarcoma - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chapman, A. L.</creatorcontrib><creatorcontrib>Bogner, Phil</creatorcontrib><creatorcontrib>Behbehani, Abbas M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chapman, A. L.</au><au>Bogner, Phil</au><au>Behbehani, Abbas M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Study of a New Human Tumor Cell Line (Rhabdomyosarcoma)</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1974-09</date><risdate>1974</risdate><volume>146</volume><issue>4</issue><spage>1087</spage><epage>1092</epage><pages>1087-1092</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
A rhabdomyosarcoma cell culture (HUS-2) was established from an 80 year old female. The cells underwent a morphologic change (at the 12-14 cell transfer) but the karyotypic pattern remained stable throughout. It was our impression that this morphologic change was not an example of transformation but simply an adaptative process of these cells to the culture conditions. The modal number was 58 with a telocentric marker chromosome before and after the morphologic alteration. Treatment with 5-Iododeoxy-uridine and 20-methylcholantrene and DEAE-D caused no karyotypic change. Electron microscopy showed masses of fibrillar material in the multinucleated giant cells and no virus particles were seen in any culture examined.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>4425030</pmid><doi>10.3181/00379727-146-38250</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1974-09, Vol.146 (4), p.1087-1092 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82557189 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Cell Line Cell Nucleus - ultrastructure Cells, Cultured Chromosomes - drug effects Deoxyuridine - pharmacology Dextrans - pharmacology Ethylamines - pharmacology Humans Iodoproteins Karyotyping Methylcholanthrene - pharmacology Microscopy, Electron Oncogenic Viruses - isolation & purification Rhabdomyosarcoma - microbiology Rhabdomyosarcoma - pathology |
| title | A Study of a New Human Tumor Cell Line (Rhabdomyosarcoma) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T17%3A18%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Study%20of%20a%20New%20Human%20Tumor%20Cell%20Line%20(Rhabdomyosarcoma)&rft.jtitle=Experimental%20biology%20and%20medicine%20(Maywood,%20N.J.)&rft.au=Chapman,%20A.%20L.&rft.date=1974-09&rft.volume=146&rft.issue=4&rft.spage=1087&rft.epage=1092&rft.pages=1087-1092&rft.issn=0037-9727&rft.eissn=1535-3699&rft_id=info:doi/10.3181/00379727-146-38250&rft_dat=%3Cproquest_cross%3E82557189%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82557189&rft_id=info:pmid/4425030&rft_sage_id=10.3181_00379727-146-38250&rfr_iscdi=true |