Rapid assays for adenylate cyclase and 3′, 5′ cyclic AMP phosphodiesterase activities. Simultaneous measurements of other pathways of ATP catabolism
This paper deals with a rapid assay of adenylate cyclase activity and 3′,5′ cyclic AMP phosphodiesterase activity which permits simultaneous measurements of other pathways of ATP catabolism. The separation of all the adenylic nucleotides was obtained by an electrophoresis on cellulose acetate 15 min...
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| Veröffentlicht in: | Analytical biochemistry 1974-12, Vol.62 (2), p.417-425 |
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| container_end_page | 425 |
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| container_title | Analytical biochemistry |
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| creator | Delaage, Michel A. Bellon, Bernard N. Cailla, Hélène L. |
| description | This paper deals with a rapid assay of adenylate cyclase activity and 3′,5′ cyclic AMP phosphodiesterase activity which permits simultaneous measurements of other pathways of ATP catabolism. The separation of all the adenylic nucleotides was obtained by an electrophoresis on cellulose acetate 15 min at 50 V/cm with a fluorescent buffer pH 8.6. Before electrophoresis, the incubation sample was added with a carrier solution of nucleotides to allow their localization under uv light, by fluorescence inhibition. Each fraction was cut and dissolved in Bray's liquid for scintillation counting. This assay method is rapid, reliable, and sensitive, it is suitable either for research or for routine and clinical purposes. |
| doi_str_mv | 10.1016/0003-2697(74)90174-2 |
| format | Article |
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This assay method is rapid, reliable, and sensitive, it is suitable either for research or for routine and clinical purposes.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Adenylyl Cyclases - analysis</subject><subject>Adenylyl Cyclases - metabolism</subject><subject>Animals</subject><subject>Cattle</subject><subject>Cellulose</subject><subject>Cyclic AMP</subject><subject>Electrophoresis</subject><subject>Fluorescence</subject><subject>Kinetics</subject><subject>Methods</subject><subject>Pancreas - enzymology</subject><subject>Phosphoric Diester Hydrolases - analysis</subject><subject>Rats</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uc1q3DAQFqUh3aZ9gxZ0Ki3EqSRLln0pLKFtAikJbXoWWmnEKtiWI8kpe8tj5DnySH2S2LtLjjnMDHzzze-H0AdKTiih1VdCSFmwqpGfJf_SECp5wV6hBSVNVZCSNK_R4pnyBr1N6YYQSrmoDtEhLyVnslmgh9968BbrlPQmYRci1hb6TaszYLMxrU6AdW9x-f_-8RiLyW9hb_Dy1xUe1iFNZj2kDHHLNdnf-TwBJ_iP78Y26x7CmHAHOo0ROuhzwsHhkNcQ8aDz-t88eUKW11fY6KxXofWpe4cOnG4TvN_HI_T3x_fr07Pi4vLn-enyojClkLnQtuZlJUALWlFLidXOmZpJWVtRNwIIk46uJKsNrYWlzDZy5XjFeOV4bQgtj9CnXd8hhttxukN1Phlo293eqmaCE8nYROQ7ookhpQhODdF3Om4UJWoWRM3fVvO3leRqK4iayz7u-4-rDuxz0V6BKf9tl4fpyDsPUSXjoTdgfQSTlQ3-5QFPMqOdyQ</recordid><startdate>197412</startdate><enddate>197412</enddate><creator>Delaage, Michel A.</creator><creator>Bellon, Bernard N.</creator><creator>Cailla, Hélène L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197412</creationdate><title>Rapid assays for adenylate cyclase and 3′, 5′ cyclic AMP phosphodiesterase activities. 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Simultaneous measurements of other pathways of ATP catabolism</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1974-12</date><risdate>1974</risdate><volume>62</volume><issue>2</issue><spage>417</spage><epage>425</epage><pages>417-425</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>This paper deals with a rapid assay of adenylate cyclase activity and 3′,5′ cyclic AMP phosphodiesterase activity which permits simultaneous measurements of other pathways of ATP catabolism. The separation of all the adenylic nucleotides was obtained by an electrophoresis on cellulose acetate 15 min at 50 V/cm with a fluorescent buffer pH 8.6. Before electrophoresis, the incubation sample was added with a carrier solution of nucleotides to allow their localization under uv light, by fluorescence inhibition. Each fraction was cut and dissolved in Bray's liquid for scintillation counting. 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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1974-12, Vol.62 (2), p.417-425 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82540722 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - metabolism Adenylyl Cyclases - analysis Adenylyl Cyclases - metabolism Animals Cattle Cellulose Cyclic AMP Electrophoresis Fluorescence Kinetics Methods Pancreas - enzymology Phosphoric Diester Hydrolases - analysis Rats |
| title | Rapid assays for adenylate cyclase and 3′, 5′ cyclic AMP phosphodiesterase activities. Simultaneous measurements of other pathways of ATP catabolism |
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