Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 Complexes
A new method for detecting human complement receptor (B) lymphocytes by utilizing rosette formation with zymosan particles treated with mouse complement (ZC) has been described in this paper. The method has been compared with rosette formation with human erythrocytes sensitized with rabbit antibody...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 1974-08, Vol.113 (2), p.531-536 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 536 |
|---|---|
| container_issue | 2 |
| container_start_page | 531 |
| container_title | The Journal of immunology (1950) |
| container_volume | 113 |
| creator | Mendes, Nelson F Miki, Silvia S Peixinho, Zulma F |
| description | A new method for detecting human complement receptor (B) lymphocytes by utilizing rosette formation with zymosan particles treated with mouse complement (ZC) has been described in this paper. The method has been compared with rosette formation with human erythrocytes sensitized with rabbit antibody and mouse complement (HEAC). T cells have been detected by rosette formation with sheep erythrocytes (E). The combined detection of both B and T lymphocytes could be performed by using as indicator a mixture of ZC and E. Mean percentage values for E and ZC were 60.5 and 15.2 by using the combined determination to study lymphocytes from 30 normal subjects. The mean percentage value for HEAC was 19.5. Only a small proportion of lymphocytes (1 to 2%) formed mixed rosettes with ZC and E. This paper further demonstrates the use of this combined indicator for the study of the distribution of B and T cells in tissue sections. |
| doi_str_mv | 10.4049/jimmunol.113.2.531 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_82454670</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>82454670</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2881-4e037cbe0f9cd1ac047725d647aed147927f6dd3d66f87801b6e898fa20028893</originalsourceid><addsrcrecordid>eNpFkE1P3DAURS1EBcPHH0BC8opdprbj2M4SplAqjYTU0g0by4lfSFAcBztRmn_fDAyweptz79U7CF1QsuaE599fGufGzrdrStM1W2cpPUArmmUkEYKIQ7QihLGESiGP0UmML4QQQRg_Qkc844JzsULjxrui6cDiHzBAOTS-w77C96MzHX7EprP4Bm9n19e-nAeIuJjxbx9hGADf-eDMW2Jqhhr_qQF6fBvmoQ57eBd_mp2Ppks2KV62-hb-QTxD3yrTRjjf31P09-72cXOfbB9-_tpcb5OSKUUTDiSVZQGkyktLTUm4lCyzgksDlnKZM1kJa1MrRKWkIrQQoHJVGbY8rlSenqKr994--NcR4qBdE0toW9OBH6NWbCdCkgVk72AZfIwBKt2Hxpkwa0r0zrX-cK0X15rpxfUSuty3j4UD-xnZy_1ar5vnemoC6OhM2y401dM0fRX9B4c1ilQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>82454670</pqid></control><display><type>article</type><title>Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 Complexes</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Mendes, Nelson F ; Miki, Silvia S ; Peixinho, Zulma F</creator><creatorcontrib>Mendes, Nelson F ; Miki, Silvia S ; Peixinho, Zulma F</creatorcontrib><description>A new method for detecting human complement receptor (B) lymphocytes by utilizing rosette formation with zymosan particles treated with mouse complement (ZC) has been described in this paper. The method has been compared with rosette formation with human erythrocytes sensitized with rabbit antibody and mouse complement (HEAC). T cells have been detected by rosette formation with sheep erythrocytes (E). The combined detection of both B and T lymphocytes could be performed by using as indicator a mixture of ZC and E. Mean percentage values for E and ZC were 60.5 and 15.2 by using the combined determination to study lymphocytes from 30 normal subjects. The mean percentage value for HEAC was 19.5. Only a small proportion of lymphocytes (1 to 2%) formed mixed rosettes with ZC and E. This paper further demonstrates the use of this combined indicator for the study of the distribution of B and T cells in tissue sections.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.113.2.531</identifier><identifier>PMID: 4546446</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Adult ; Animals ; B-Lymphocytes - immunology ; Complement System Proteins ; Erythrocytes - immunology ; Female ; Humans ; Immune Adherence Reaction ; Immune Sera ; Immunity, Cellular ; Indicators and Reagents ; Lymph Nodes - immunology ; Male ; Methods ; Middle Aged ; Rabbits - immunology ; Sheep - immunology ; T-Lymphocytes - immunology ; Zymosan</subject><ispartof>The Journal of immunology (1950), 1974-08, Vol.113 (2), p.531-536</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2881-4e037cbe0f9cd1ac047725d647aed147927f6dd3d66f87801b6e898fa20028893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4546446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mendes, Nelson F</creatorcontrib><creatorcontrib>Miki, Silvia S</creatorcontrib><creatorcontrib>Peixinho, Zulma F</creatorcontrib><title>Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 Complexes</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>A new method for detecting human complement receptor (B) lymphocytes by utilizing rosette formation with zymosan particles treated with mouse complement (ZC) has been described in this paper. The method has been compared with rosette formation with human erythrocytes sensitized with rabbit antibody and mouse complement (HEAC). T cells have been detected by rosette formation with sheep erythrocytes (E). The combined detection of both B and T lymphocytes could be performed by using as indicator a mixture of ZC and E. Mean percentage values for E and ZC were 60.5 and 15.2 by using the combined determination to study lymphocytes from 30 normal subjects. The mean percentage value for HEAC was 19.5. Only a small proportion of lymphocytes (1 to 2%) formed mixed rosettes with ZC and E. This paper further demonstrates the use of this combined indicator for the study of the distribution of B and T cells in tissue sections.</description><subject>Adult</subject><subject>Animals</subject><subject>B-Lymphocytes - immunology</subject><subject>Complement System Proteins</subject><subject>Erythrocytes - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Immune Adherence Reaction</subject><subject>Immune Sera</subject><subject>Immunity, Cellular</subject><subject>Indicators and Reagents</subject><subject>Lymph Nodes - immunology</subject><subject>Male</subject><subject>Methods</subject><subject>Middle Aged</subject><subject>Rabbits - immunology</subject><subject>Sheep - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Zymosan</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1P3DAURS1EBcPHH0BC8opdprbj2M4SplAqjYTU0g0by4lfSFAcBztRmn_fDAyweptz79U7CF1QsuaE599fGufGzrdrStM1W2cpPUArmmUkEYKIQ7QihLGESiGP0UmML4QQQRg_Qkc844JzsULjxrui6cDiHzBAOTS-w77C96MzHX7EprP4Bm9n19e-nAeIuJjxbx9hGADf-eDMW2Jqhhr_qQF6fBvmoQ57eBd_mp2Ppks2KV62-hb-QTxD3yrTRjjf31P09-72cXOfbB9-_tpcb5OSKUUTDiSVZQGkyktLTUm4lCyzgksDlnKZM1kJa1MrRKWkIrQQoHJVGbY8rlSenqKr994--NcR4qBdE0toW9OBH6NWbCdCkgVk72AZfIwBKt2Hxpkwa0r0zrX-cK0X15rpxfUSuty3j4UD-xnZy_1ar5vnemoC6OhM2y401dM0fRX9B4c1ilQ</recordid><startdate>197408</startdate><enddate>197408</enddate><creator>Mendes, Nelson F</creator><creator>Miki, Silvia S</creator><creator>Peixinho, Zulma F</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197408</creationdate><title>Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 Complexes</title><author>Mendes, Nelson F ; Miki, Silvia S ; Peixinho, Zulma F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2881-4e037cbe0f9cd1ac047725d647aed147927f6dd3d66f87801b6e898fa20028893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Adult</topic><topic>Animals</topic><topic>B-Lymphocytes - immunology</topic><topic>Complement System Proteins</topic><topic>Erythrocytes - immunology</topic><topic>Female</topic><topic>Humans</topic><topic>Immune Adherence Reaction</topic><topic>Immune Sera</topic><topic>Immunity, Cellular</topic><topic>Indicators and Reagents</topic><topic>Lymph Nodes - immunology</topic><topic>Male</topic><topic>Methods</topic><topic>Middle Aged</topic><topic>Rabbits - immunology</topic><topic>Sheep - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Zymosan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mendes, Nelson F</creatorcontrib><creatorcontrib>Miki, Silvia S</creatorcontrib><creatorcontrib>Peixinho, Zulma F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mendes, Nelson F</au><au>Miki, Silvia S</au><au>Peixinho, Zulma F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 Complexes</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1974-08</date><risdate>1974</risdate><volume>113</volume><issue>2</issue><spage>531</spage><epage>536</epage><pages>531-536</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>A new method for detecting human complement receptor (B) lymphocytes by utilizing rosette formation with zymosan particles treated with mouse complement (ZC) has been described in this paper. The method has been compared with rosette formation with human erythrocytes sensitized with rabbit antibody and mouse complement (HEAC). T cells have been detected by rosette formation with sheep erythrocytes (E). The combined detection of both B and T lymphocytes could be performed by using as indicator a mixture of ZC and E. Mean percentage values for E and ZC were 60.5 and 15.2 by using the combined determination to study lymphocytes from 30 normal subjects. The mean percentage value for HEAC was 19.5. Only a small proportion of lymphocytes (1 to 2%) formed mixed rosettes with ZC and E. This paper further demonstrates the use of this combined indicator for the study of the distribution of B and T cells in tissue sections.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>4546446</pmid><doi>10.4049/jimmunol.113.2.531</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1974-08, Vol.113 (2), p.531-536 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82454670 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Animals B-Lymphocytes - immunology Complement System Proteins Erythrocytes - immunology Female Humans Immune Adherence Reaction Immune Sera Immunity, Cellular Indicators and Reagents Lymph Nodes - immunology Male Methods Middle Aged Rabbits - immunology Sheep - immunology T-Lymphocytes - immunology Zymosan |
| title | Combined Detection of Human T and B Lymphocytes by Rosette Formation with Sheep Erythrocytes and Zymosan-C3 Complexes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T16%3A42%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Combined%20Detection%20of%20Human%20T%20and%20B%20Lymphocytes%20by%20Rosette%20Formation%20with%20Sheep%20Erythrocytes%20and%20Zymosan-C3%20Complexes&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Mendes,%20Nelson%20F&rft.date=1974-08&rft.volume=113&rft.issue=2&rft.spage=531&rft.epage=536&rft.pages=531-536&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.113.2.531&rft_dat=%3Cproquest_cross%3E82454670%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=82454670&rft_id=info:pmid/4546446&rfr_iscdi=true |