Reduction of insolubilized fibrinogen

Insolubilization of fibrinogen is achieved by covalent fixation onto cyanogen bromide activated agarose. When insolubilized fibrinogen (FG-ag) is reduced in order to separate the fibrinogen molecule into its chains, disc-electrophoretic patterns reveal that the (‘A’) α-chains remain with the insoluble agarose whereas the (B) β-chains and the γ-chains appear in the supernatant. It is assumed that covalent fixation of fibrinogen to agarose almost exclusively takes place at the (‘A’) α-chain and that the (‘A’) α-chain is located at the surface of the fibrinogen molecule.