Ultramicro (nanoliter range) determination of calcium concentration (10 −3m) by atomic absorption
An original ultramicro atomic absorption spectrophotometer is reported. Picomole amounts of calcium, in the nanoliter range volume were determined. Reproducibility, linearity, and recovery were statisfactory. No interference effects were observed from biological concentrations of Na, K, Mg, and Pi....
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| Veröffentlicht in: | Analytical biochemistry 1974-08, Vol.60 (2), p.449-454 |
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| container_title | Analytical biochemistry |
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| creator | Kuntziger, H. Antonetti, A. Couette, S. Coureau, C. Amiel, C. |
| description | An original ultramicro atomic absorption spectrophotometer is reported. Picomole amounts of calcium, in the nanoliter range volume were determined. Reproducibility, linearity, and recovery were statisfactory. No interference effects were observed from biological concentrations of Na, K, Mg, and Pi. Urea decreased the signal at concentrations above 0.1
m.
Determinations were performed on late proximal renal tubular fluid and plasma ultrafiltrate samples obtained from three groups of rats: normal, acutely parathyroidectomized, and parathyroidectomized 2 days prior to the experiment. The results indicate a significantly higher tubular fluid to plasma ultrafiltrate calcium gradient in the acutely parathyroidectomized group.
This spectrophotometer has several advantages, and disadvantages, as compared to the electron probe. |
| doi_str_mv | 10.1016/0003-2697(74)90254-1 |
| format | Article |
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m.
Determinations were performed on late proximal renal tubular fluid and plasma ultrafiltrate samples obtained from three groups of rats: normal, acutely parathyroidectomized, and parathyroidectomized 2 days prior to the experiment. The results indicate a significantly higher tubular fluid to plasma ultrafiltrate calcium gradient in the acutely parathyroidectomized group.
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m.
Determinations were performed on late proximal renal tubular fluid and plasma ultrafiltrate samples obtained from three groups of rats: normal, acutely parathyroidectomized, and parathyroidectomized 2 days prior to the experiment. The results indicate a significantly higher tubular fluid to plasma ultrafiltrate calcium gradient in the acutely parathyroidectomized group.
This spectrophotometer has several advantages, and disadvantages, as compared to the electron probe.</description><subject>Animals</subject><subject>Body Fluids - analysis</subject><subject>Calcium - analysis</subject><subject>Calcium - blood</subject><subject>Calcium - metabolism</subject><subject>Calcium Chloride - analysis</subject><subject>Electron Probe Microanalysis</subject><subject>Evaluation Studies as Topic</subject><subject>Kidney Tubules, Proximal - analysis</subject><subject>Magnesium - analysis</subject><subject>Methods</subject><subject>Microchemistry</subject><subject>Osmolar Concentration</subject><subject>Parathyroid Glands - physiology</subject><subject>Phosphates</subject><subject>Potassium</subject><subject>Rats</subject><subject>Sodium</subject><subject>Spectrophotometry, Atomic</subject><subject>Ultrafiltration</subject><subject>Urea</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotVbfQCEraRejySRz2whSvEHBjV2HTHIikZlJTaZC38C1j-iTmGlLl65ODv8lnA-hS0puKKH5LSGEJWleFdOCzyqSZjyhR2hMSZUnhJHqGI0PllN0FsIHIZTyLB-hES95fPAxUsum97K1yjs87WTnGtuDx1527zDDGuLS2k721nXYGaxko-y6xcp1CrqY3ApTSvDv9w9rZ7jeYNm72IdlHZxfDfo5OjGyCXCxnxO0fHx4mz8ni9enl_n9IlEsK_qkhBRywqCqU2mUyWWmVAmGlYzXTNLKUMlUlgJoqU2a17nRWpOqKAtSqJTWbIKud70r7z7XEHrR2qCgaWQHbh1EmXJWlVkZjXxnjFeH4MGIlbet9BtBiRjYigGcGMCJgostW0Fj7Grfv65b0IfQHmbU73Y6xCO_LHgRlIUISlsPqhfa2f8_-ANUC4ql</recordid><startdate>197408</startdate><enddate>197408</enddate><creator>Kuntziger, H.</creator><creator>Antonetti, A.</creator><creator>Couette, S.</creator><creator>Coureau, C.</creator><creator>Amiel, C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197408</creationdate><title>Ultramicro (nanoliter range) determination of calcium concentration (10 −3m) by atomic absorption</title><author>Kuntziger, H. ; Antonetti, A. ; Couette, S. ; Coureau, C. ; Amiel, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-8e2e603e9b2afcf6a5cc8ef3834b3a19f1a3c52eedadf26b6fddd0978707c21b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Animals</topic><topic>Body Fluids - analysis</topic><topic>Calcium - analysis</topic><topic>Calcium - blood</topic><topic>Calcium - metabolism</topic><topic>Calcium Chloride - analysis</topic><topic>Electron Probe Microanalysis</topic><topic>Evaluation Studies as Topic</topic><topic>Kidney Tubules, Proximal - analysis</topic><topic>Magnesium - analysis</topic><topic>Methods</topic><topic>Microchemistry</topic><topic>Osmolar Concentration</topic><topic>Parathyroid Glands - physiology</topic><topic>Phosphates</topic><topic>Potassium</topic><topic>Rats</topic><topic>Sodium</topic><topic>Spectrophotometry, Atomic</topic><topic>Ultrafiltration</topic><topic>Urea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuntziger, H.</creatorcontrib><creatorcontrib>Antonetti, A.</creatorcontrib><creatorcontrib>Couette, S.</creatorcontrib><creatorcontrib>Coureau, C.</creatorcontrib><creatorcontrib>Amiel, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuntziger, H.</au><au>Antonetti, A.</au><au>Couette, S.</au><au>Coureau, C.</au><au>Amiel, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultramicro (nanoliter range) determination of calcium concentration (10 −3m) by atomic absorption</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1974-08</date><risdate>1974</risdate><volume>60</volume><issue>2</issue><spage>449</spage><epage>454</epage><pages>449-454</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>An original ultramicro atomic absorption spectrophotometer is reported. Picomole amounts of calcium, in the nanoliter range volume were determined. Reproducibility, linearity, and recovery were statisfactory. No interference effects were observed from biological concentrations of Na, K, Mg, and Pi. Urea decreased the signal at concentrations above 0.1
m.
Determinations were performed on late proximal renal tubular fluid and plasma ultrafiltrate samples obtained from three groups of rats: normal, acutely parathyroidectomized, and parathyroidectomized 2 days prior to the experiment. The results indicate a significantly higher tubular fluid to plasma ultrafiltrate calcium gradient in the acutely parathyroidectomized group.
This spectrophotometer has several advantages, and disadvantages, as compared to the electron probe.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4844564</pmid><doi>10.1016/0003-2697(74)90254-1</doi><tpages>6</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1974-08, Vol.60 (2), p.449-454 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Body Fluids - analysis Calcium - analysis Calcium - blood Calcium - metabolism Calcium Chloride - analysis Electron Probe Microanalysis Evaluation Studies as Topic Kidney Tubules, Proximal - analysis Magnesium - analysis Methods Microchemistry Osmolar Concentration Parathyroid Glands - physiology Phosphates Potassium Rats Sodium Spectrophotometry, Atomic Ultrafiltration Urea |
| title | Ultramicro (nanoliter range) determination of calcium concentration (10 −3m) by atomic absorption |
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