Measurement of protein by spectrophotometry at 205 nm
A method is described for the measurement of protein concentration by using the peptide bond absorption at 205 nm. ϵ 205 is estimated, allowing for the absorption due to Trp and Tyr residues, by measuring the absorbance at 280 nm as well as at 205 nm. The estimated ϵ 205 is compared with the actual...
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| Veröffentlicht in: | Analytical biochemistry 1974-05, Vol.59 (1), p.277-282 |
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| container_end_page | 282 |
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| container_start_page | 277 |
| container_title | Analytical biochemistry |
| container_volume | 59 |
| creator | Scopes, R.K. |
| description | A method is described for the measurement of protein concentration by using the peptide bond absorption at 205 nm.
ϵ
205 is estimated, allowing for the absorption due to Trp and Tyr residues, by measuring the absorbance at 280 nm as well as at 205 nm. The estimated
ϵ
205 is compared with the actual
ϵ
205 for a number of proteins, the mean error being less than 2%. This is about three times better than using an average
ϵ
205
1 mg/ml of 31 and approaches the range of experimental error inherent in any method of protein estimation. |
| doi_str_mv | 10.1016/0003-2697(74)90034-7 |
| format | Article |
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ϵ
205 is estimated, allowing for the absorption due to Trp and Tyr residues, by measuring the absorbance at 280 nm as well as at 205 nm. The estimated
ϵ
205 is compared with the actual
ϵ
205 for a number of proteins, the mean error being less than 2%. This is about three times better than using an average
ϵ
205
1 mg/ml of 31 and approaches the range of experimental error inherent in any method of protein estimation.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(74)90034-7</identifier><identifier>PMID: 4407487</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Evaluation Studies as Topic ; Mathematics ; Methods ; Muramidase - analysis ; Proteins - analysis ; Serum Albumin, Bovine - analysis ; Species Specificity ; Spectrophotometry, Ultraviolet ; Tryptophan ; Tyrosine</subject><ispartof>Analytical biochemistry, 1974-05, Vol.59 (1), p.277-282</ispartof><rights>1974</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-740343412ebd815b547b79a0b450ae696a6a993c53309068544029bedf9f32fe3</citedby><cites>FETCH-LOGICAL-c423t-740343412ebd815b547b79a0b450ae696a6a993c53309068544029bedf9f32fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(74)90034-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4407487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scopes, R.K.</creatorcontrib><title>Measurement of protein by spectrophotometry at 205 nm</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A method is described for the measurement of protein concentration by using the peptide bond absorption at 205 nm.
ϵ
205 is estimated, allowing for the absorption due to Trp and Tyr residues, by measuring the absorbance at 280 nm as well as at 205 nm. The estimated
ϵ
205 is compared with the actual
ϵ
205 for a number of proteins, the mean error being less than 2%. This is about three times better than using an average
ϵ
205
1 mg/ml of 31 and approaches the range of experimental error inherent in any method of protein estimation.</description><subject>Animals</subject><subject>Cattle</subject><subject>Evaluation Studies as Topic</subject><subject>Mathematics</subject><subject>Methods</subject><subject>Muramidase - analysis</subject><subject>Proteins - analysis</subject><subject>Serum Albumin, Bovine - analysis</subject><subject>Species Specificity</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Tryptophan</subject><subject>Tyrosine</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRaq3-A4WcRA_R2exX9iJI8QsqXvS8bJIJRpps3N0K_fdubenR0zDM-8688xByTuGGApW3AMDyQmp1pfi1Tg3P1QGZUtAyBwb6kEz3kmNyEsIXAKVcyAmZcA6Kl2pKxCvasPLY4xAz12ajdxG7IavWWRixjt6Nny66HqNfZzZmBYhs6E_JUWuXAc92dUY-Hh_e58_54u3pZX6_yGtesJgrnkIxTgusmpKKSnBVKW2h4gIsSi2ttFqzWrAUF2QpUqxCV9i0umVFi2xGLrd7U6zvFYZo-i7UuFzaAd0qmLLgwDUtk5BvhbV3IXhszei73vq1oWA2tMwGhdmgMIqbP1pGJdvFbv-q6rHZm3Z40vxuO8f05E-H3oS6w6HGpvMJjmlc9_-BXxnZdrk</recordid><startdate>197405</startdate><enddate>197405</enddate><creator>Scopes, R.K.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197405</creationdate><title>Measurement of protein by spectrophotometry at 205 nm</title><author>Scopes, R.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-740343412ebd815b547b79a0b450ae696a6a993c53309068544029bedf9f32fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Evaluation Studies as Topic</topic><topic>Mathematics</topic><topic>Methods</topic><topic>Muramidase - analysis</topic><topic>Proteins - analysis</topic><topic>Serum Albumin, Bovine - analysis</topic><topic>Species Specificity</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Tryptophan</topic><topic>Tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scopes, R.K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scopes, R.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of protein by spectrophotometry at 205 nm</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1974-05</date><risdate>1974</risdate><volume>59</volume><issue>1</issue><spage>277</spage><epage>282</epage><pages>277-282</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A method is described for the measurement of protein concentration by using the peptide bond absorption at 205 nm.
ϵ
205 is estimated, allowing for the absorption due to Trp and Tyr residues, by measuring the absorbance at 280 nm as well as at 205 nm. The estimated
ϵ
205 is compared with the actual
ϵ
205 for a number of proteins, the mean error being less than 2%. This is about three times better than using an average
ϵ
205
1 mg/ml of 31 and approaches the range of experimental error inherent in any method of protein estimation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4407487</pmid><doi>10.1016/0003-2697(74)90034-7</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1974-05, Vol.59 (1), p.277-282 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82404918 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cattle Evaluation Studies as Topic Mathematics Methods Muramidase - analysis Proteins - analysis Serum Albumin, Bovine - analysis Species Specificity Spectrophotometry, Ultraviolet Tryptophan Tyrosine |
| title | Measurement of protein by spectrophotometry at 205 nm |
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