Ultrastructure of the crystalline inclusion induced by tobacco etch virus visualized by freeze-etching
Structural subunits of the crystalline inclusion induced by tobacco etch virus were clearly resolved when inclusions were examined both in situ and in isolated preparations by freeze-etch electron microscopy. Data supported the contention that the crystal substructure is organized into laminae and t...
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Veröffentlicht in: | Journal of ultrastructure research 1974-01, Vol.48 (1), p.138-152 |
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creator | McDonald, J.G. Hiebert, Ernest |
description | Structural subunits of the crystalline inclusion induced by tobacco etch virus were clearly resolved when inclusions were examined both
in situ and in isolated preparations by freeze-etch electron microscopy. Data supported the contention that the crystal substructure is organized into laminae and that these laminae assemble into crystals by stacking vertically. The substructure of the laminae, revealed by examining isolated inclusions, appeared to consist of a single layer of subunits packed in rectangular array. When inclusions were cross-fractured
in situ, subunits were evident in rectangular and semihexagonal arrays. Based on these observations a model is proposed for the arrangement of subunits in the crystal. |
doi_str_mv | 10.1016/S0022-5320(74)80050-X |
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in situ and in isolated preparations by freeze-etch electron microscopy. Data supported the contention that the crystal substructure is organized into laminae and that these laminae assemble into crystals by stacking vertically. The substructure of the laminae, revealed by examining isolated inclusions, appeared to consist of a single layer of subunits packed in rectangular array. When inclusions were cross-fractured
in situ, subunits were evident in rectangular and semihexagonal arrays. Based on these observations a model is proposed for the arrangement of subunits in the crystal.</description><subject>Crystallization</subject><subject>Datura stramonium</subject><subject>Freeze Etching</subject><subject>Inclusion Bodies, Viral</subject><subject>Microscopy, Electron</subject><subject>Plant Viruses</subject><subject>RNA Viruses</subject><subject>Virus Replication</subject><issn>0022-5320</issn><issn>1878-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVpSTdpf0KoT6U5uJWssT0-lRL6BYEe0oXchCyNExWvleojsPn11cZLrr3MDLzPjMTD2LngHwUX3adrzpumbmXDP_RwgZy3vL55wTYCe6wbCe1LtnlGXrPTGP9wLoVAOGEngBIQcMOm7ZyCjilkk3Kgyk9VuqPKhH1Mep7dQpVbzJyj80uZbDZkq3FfJT9qY3xFydxVDy7kWGrMenaPKzAFokeqD7lbbt-wV5OeI7099jO2_fb19-WP-urX95-XX65qIxFSTWigQ0uWU9ujxc6ShgEn0Khx6GUH3SDRDpoP3QhjOwnewdBQN1ije6PlGXu_3r0P_m-mmNTORUPzrBfyOSpsgAsYeAHbFTTBxxhoUvfB7XTYK8HVwa968qsO8lQP6smvuil758cH8rgj-7x1FFryd2s-aa_0bXBRba8bLmRR3wuQfSE-rwQVDw-OgorG0VK8ukAmKevdf_7wD0oklbE</recordid><startdate>19740101</startdate><enddate>19740101</enddate><creator>McDonald, J.G.</creator><creator>Hiebert, Ernest</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19740101</creationdate><title>Ultrastructure of the crystalline inclusion induced by tobacco etch virus visualized by freeze-etching</title><author>McDonald, J.G. ; Hiebert, Ernest</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-e8c468ded0e578d86dea498f4a8a8973646938d9a096b4b5f106492e69dca7ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Crystallization</topic><topic>Datura stramonium</topic><topic>Freeze Etching</topic><topic>Inclusion Bodies, Viral</topic><topic>Microscopy, Electron</topic><topic>Plant Viruses</topic><topic>RNA Viruses</topic><topic>Virus Replication</topic><toplevel>online_resources</toplevel><creatorcontrib>McDonald, J.G.</creatorcontrib><creatorcontrib>Hiebert, Ernest</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ultrastructure research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McDonald, J.G.</au><au>Hiebert, Ernest</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructure of the crystalline inclusion induced by tobacco etch virus visualized by freeze-etching</atitle><jtitle>Journal of ultrastructure research</jtitle><addtitle>J Ultrastruct Res</addtitle><date>1974-01-01</date><risdate>1974</risdate><volume>48</volume><issue>1</issue><spage>138</spage><epage>152</epage><pages>138-152</pages><issn>0022-5320</issn><eissn>1878-2345</eissn><abstract>Structural subunits of the crystalline inclusion induced by tobacco etch virus were clearly resolved when inclusions were examined both
in situ and in isolated preparations by freeze-etch electron microscopy. Data supported the contention that the crystal substructure is organized into laminae and that these laminae assemble into crystals by stacking vertically. The substructure of the laminae, revealed by examining isolated inclusions, appeared to consist of a single layer of subunits packed in rectangular array. When inclusions were cross-fractured
in situ, subunits were evident in rectangular and semihexagonal arrays. Based on these observations a model is proposed for the arrangement of subunits in the crystal.</abstract><cop>United States</cop><pub>Elsevier B.V</pub><pmid>4834848</pmid><doi>10.1016/S0022-5320(74)80050-X</doi><tpages>15</tpages></addata></record> |
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source | MEDLINE; Alma/SFX Local Collection |
subjects | Crystallization Datura stramonium Freeze Etching Inclusion Bodies, Viral Microscopy, Electron Plant Viruses RNA Viruses Virus Replication |
title | Ultrastructure of the crystalline inclusion induced by tobacco etch virus visualized by freeze-etching |
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