PEI-cellulose thin-layer chromatography product studies of the creatine kinase and pyruvate kinase reactions
Detailed methods for polyethylenimine-cellulose thin-layer chromatographic determination of the reactants and products of the creatine kinase and pyruvate kinase reactions are presented. The technique has been developed as a highly sensitive method both for estimation of relative initial rates affor...
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| Veröffentlicht in: | Analytical biochemistry 1974-04, Vol.58 (2), p.525-533 |
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| container_end_page | 533 |
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| container_issue | 2 |
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| container_title | Analytical biochemistry |
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| creator | Rowley, Gerald L. Kenyon, George L. |
| description | Detailed methods for polyethylenimine-cellulose thin-layer chromatographic determination of the reactants and products of the creatine kinase and pyruvate kinase reactions are presented. The technique has been developed as a highly sensitive method both for estimation of relative initial rates afforded by creatine and creatine analogs in the creatine kinase reaction and also for estimation of trace amounts of inorganic phosphate and ADP contamination in samples of ATP. |
| doi_str_mv | 10.1016/0003-2697(74)90220-6 |
| format | Article |
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The technique has been developed as a highly sensitive method both for estimation of relative initial rates afforded by creatine and creatine analogs in the creatine kinase reaction and also for estimation of trace amounts of inorganic phosphate and ADP contamination in samples of ATP.</description><subject>Adenosine Diphosphate - analysis</subject><subject>Adenosine Triphosphate - analysis</subject><subject>Cellulose</subject><subject>Chromatography, Ion Exchange</subject><subject>Chromatography, Thin Layer</subject><subject>Creatine - analysis</subject><subject>Creatine Kinase - metabolism</subject><subject>Imines</subject><subject>Kinetics</subject><subject>Methods</subject><subject>Microchemistry</subject><subject>Phosphates - analysis</subject><subject>Polyethylenes</subject><subject>Pyruvate Kinase - metabolism</subject><subject>Structure-Activity Relationship</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9PGzEQxa2qCFLgG4C0p6o9bBn_We_6glRFQJGQ4ABny7FnG7ebdbC9SPn2OCTKkdNI837zZuYRckHhFwUqrwCA10yq9kcrfipgDGr5hcwoKFkDB_WVzA7ICfmW0j8ASkUjj8mx6FjLlZiR4enmvrY4DNMQElZ56cd6MBuMlV3GsDI5_I1mvdxU6xjcZHOV8uQ8pir0BcbKRjTZj1j996MpBmZ01XoTpzeTD72C2OzDmM7IUW-GhOf7ekpebm-e53_qh8e7-_nvh9ryps110wjVOsEZk6Yc2oPivVKua4B3skfmLDc9M9QocB2n0FjDirjocSGUZIqfku8733L064Qp65VP2yfNiGFKumO8U0p1BRQ70MaQUsRer6NfmbjRFPQ2ZL1NUG8T1K3QHyFrWcYu9_7TYoXuMLRPtejXOx3Lk28eo07W42jR-Yg2axf85wveAekPjIw</recordid><startdate>197404</startdate><enddate>197404</enddate><creator>Rowley, Gerald L.</creator><creator>Kenyon, George L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197404</creationdate><title>PEI-cellulose thin-layer chromatography product studies of the creatine kinase and pyruvate kinase reactions</title><author>Rowley, Gerald L. ; Kenyon, George L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-55497d43226a482f093f99d850386fe2dc3af2a1a90d83105ca2850bfeb496293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Adenosine Diphosphate - analysis</topic><topic>Adenosine Triphosphate - analysis</topic><topic>Cellulose</topic><topic>Chromatography, Ion Exchange</topic><topic>Chromatography, Thin Layer</topic><topic>Creatine - analysis</topic><topic>Creatine Kinase - metabolism</topic><topic>Imines</topic><topic>Kinetics</topic><topic>Methods</topic><topic>Microchemistry</topic><topic>Phosphates - analysis</topic><topic>Polyethylenes</topic><topic>Pyruvate Kinase - metabolism</topic><topic>Structure-Activity Relationship</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rowley, Gerald L.</creatorcontrib><creatorcontrib>Kenyon, George L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rowley, Gerald L.</au><au>Kenyon, George L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PEI-cellulose thin-layer chromatography product studies of the creatine kinase and pyruvate kinase reactions</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1974-04</date><risdate>1974</risdate><volume>58</volume><issue>2</issue><spage>525</spage><epage>533</epage><pages>525-533</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Detailed methods for polyethylenimine-cellulose thin-layer chromatographic determination of the reactants and products of the creatine kinase and pyruvate kinase reactions are presented. 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| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1974-04, Vol.58 (2), p.525-533 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adenosine Diphosphate - analysis Adenosine Triphosphate - analysis Cellulose Chromatography, Ion Exchange Chromatography, Thin Layer Creatine - analysis Creatine Kinase - metabolism Imines Kinetics Methods Microchemistry Phosphates - analysis Polyethylenes Pyruvate Kinase - metabolism Structure-Activity Relationship |
| title | PEI-cellulose thin-layer chromatography product studies of the creatine kinase and pyruvate kinase reactions |
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