Tunicamycin — An inhibitor of yeast glycoprotein synthesis
Tunicamycin, a glucosamine-containing antibiotic, halted synthesis of the external glycoproteins invertase, acid phosphatase and mannan by yeast protoplasts within 30 min; formation of two intracellular proteins, alpha-glucosidase and alkaline phosphatase, and of glucan continued at the control rate...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1974-05, Vol.58 (1), p.287-295 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kuo, S.-C. Lampen, J.O. |
| description | Tunicamycin, a glucosamine-containing antibiotic, halted synthesis of the external glycoproteins invertase, acid phosphatase and mannan by yeast protoplasts within 30 min; formation of two intracellular proteins, alpha-glucosidase and alkaline phosphatase, and of glucan continued at the control rate for at least 60–80 min. No accumulation of mannan-free acid phosphatase or invertase was evident in treated cells. Utilization of hexoses and incorporation of
14C-amino acids into protein were not affected. Incorporation of
3H-glucosamine into trichloroacetic acid-insoluble products was only partially reduced. In yeast tunicamycin acts primarily as an inhibitor of glycoprotein synthesis and not of general glucosamine metabolism. |
| doi_str_mv | 10.1016/0006-291X(74)90925-5 |
| format | Article |
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14C-amino acids into protein were not affected. Incorporation of
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14C-amino acids into protein were not affected. Incorporation of
3H-glucosamine into trichloroacetic acid-insoluble products was only partially reduced. In yeast tunicamycin acts primarily as an inhibitor of glycoprotein synthesis and not of general glucosamine metabolism.</description><subject>Acid Phosphatase - biosynthesis</subject><subject>Alkaline Phosphatase - biosynthesis</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Carbon Radioisotopes</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Fungal Proteins - biosynthesis</subject><subject>Glucosamine - metabolism</subject><subject>Glucosamine - pharmacology</subject><subject>Glucosidases - biosynthesis</subject><subject>Glycogen - biosynthesis</subject><subject>Glycoproteins - biosynthesis</subject><subject>Kinetics</subject><subject>Mannose - metabolism</subject><subject>Polysaccharides - biosynthesis</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Sucrase - biosynthesis</subject><subject>Time Factors</subject><subject>Tritium</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKw0AUhgdRaq2-gUJWoovo3JMBEUrxBgU3FdwNyeSMHcmlziRCdj6ET-iTmNrSpauz-C_nnA-hU4KvCCbyGmMsY6rI60XCLxVWVMRiD40JVjimBPN9NN5ZDtFRCO8YE8KlGqERFyrlnI_RzaKrncmq3rg6-vn6jqZ15Oqly13b-KixUQ9ZaKO3sjfNyjctDLbQ1-0SggvH6MBmZYCT7Zygl_u7xewxnj8_PM2m89hwnLYxzS1TQnEpEoOBS14waxJFFWOySIEWVFhCqGGMmtxanAElOZHK5JRJgQs2Qeeb3uGCjw5CqysXDJRlVkPTBZ1SlkqWJIORb4zGNyF4sHrlXZX5XhOs19D0moheE9EJ13_QtBhiZ9v-Lq-g2IW2lAb9dqPD8OSnA6-DcVAbKJwH0-qicf8v-AX3CHv-</recordid><startdate>19740507</startdate><enddate>19740507</enddate><creator>Kuo, S.-C.</creator><creator>Lampen, J.O.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19740507</creationdate><title>Tunicamycin — An inhibitor of yeast glycoprotein synthesis</title><author>Kuo, S.-C. ; Lampen, J.O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-2bf39594657c0e464d3fc7929336d8e2d25f112c332cbff0ae21b169cb23650d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Acid Phosphatase - biosynthesis</topic><topic>Alkaline Phosphatase - biosynthesis</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Carbon Radioisotopes</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Fungal Proteins - biosynthesis</topic><topic>Glucosamine - metabolism</topic><topic>Glucosamine - pharmacology</topic><topic>Glucosidases - biosynthesis</topic><topic>Glycogen - biosynthesis</topic><topic>Glycoproteins - biosynthesis</topic><topic>Kinetics</topic><topic>Mannose - metabolism</topic><topic>Polysaccharides - biosynthesis</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Sucrase - biosynthesis</topic><topic>Time Factors</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuo, S.-C.</creatorcontrib><creatorcontrib>Lampen, J.O.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuo, S.-C.</au><au>Lampen, J.O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tunicamycin — An inhibitor of yeast glycoprotein synthesis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1974-05-07</date><risdate>1974</risdate><volume>58</volume><issue>1</issue><spage>287</spage><epage>295</epage><pages>287-295</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Tunicamycin, a glucosamine-containing antibiotic, halted synthesis of the external glycoproteins invertase, acid phosphatase and mannan by yeast protoplasts within 30 min; formation of two intracellular proteins, alpha-glucosidase and alkaline phosphatase, and of glucan continued at the control rate for at least 60–80 min. No accumulation of mannan-free acid phosphatase or invertase was evident in treated cells. Utilization of hexoses and incorporation of
14C-amino acids into protein were not affected. Incorporation of
3H-glucosamine into trichloroacetic acid-insoluble products was only partially reduced. In yeast tunicamycin acts primarily as an inhibitor of glycoprotein synthesis and not of general glucosamine metabolism.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4598444</pmid><doi>10.1016/0006-291X(74)90925-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1974-05, Vol.58 (1), p.287-295 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acid Phosphatase - biosynthesis Alkaline Phosphatase - biosynthesis Anti-Bacterial Agents - pharmacology Carbon Radioisotopes Chromatography, DEAE-Cellulose Fungal Proteins - biosynthesis Glucosamine - metabolism Glucosamine - pharmacology Glucosidases - biosynthesis Glycogen - biosynthesis Glycoproteins - biosynthesis Kinetics Mannose - metabolism Polysaccharides - biosynthesis Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - metabolism Sucrase - biosynthesis Time Factors Tritium |
| title | Tunicamycin — An inhibitor of yeast glycoprotein synthesis |
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