Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast
The antibiotic cerulenin inhibited the incorporation of 14C-acetyl-CoA by 67% at a concentration of 9 × 10 −6 M but not that of 14C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of Saccharomyces cerevisiae. Cerulenin markedly inhibited the activity of partially purified HMG-CoA s...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1974-04, Vol.57 (4), p.1119-1124 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 57 |
| creator | Ohno, T. Kesado, T. Awaya, J. Ōmura, S. |
| description | The antibiotic cerulenin inhibited the incorporation of
14C-acetyl-CoA by 67% at a concentration of 9 × 10
−6 M but not that of
14C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of
Saccharomyces cerevisiae. Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity. |
| doi_str_mv | 10.1016/0006-291X(74)90812-2 |
| format | Article |
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14C-acetyl-CoA by 67% at a concentration of 9 × 10
−6 M but not that of
14C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of
Saccharomyces cerevisiae. Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(74)90812-2</identifier><identifier>PMID: 4598335</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetoacetates ; Acetyl Coenzyme A - metabolism ; Acetyltransferases - metabolism ; Antifungal Agents - pharmacology ; Carbon Radioisotopes ; Cell-Free System ; Coenzyme A ; Ethers, Cyclic - pharmacology ; Fatty Acids, Unsaturated - pharmacology ; Glutarates ; Mevalonic Acid - metabolism ; Oxo-Acid-Lyases - antagonists & inhibitors ; Phytosterols - biosynthesis ; plant biochemistry ; plant physiology ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - enzymology ; Spectrophotometry, Ultraviolet ; Time Factors</subject><ispartof>Biochemical and biophysical research communications, 1974-04, Vol.57 (4), p.1119-1124</ispartof><rights>1974</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-8ba3e8b5dc078948b395cc23e0392ee175e748f463a9982187d70672242403ca3</citedby><cites>FETCH-LOGICAL-c381t-8ba3e8b5dc078948b395cc23e0392ee175e748f463a9982187d70672242403ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X74908122$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4598335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohno, T.</creatorcontrib><creatorcontrib>Kesado, T.</creatorcontrib><creatorcontrib>Awaya, J.</creatorcontrib><creatorcontrib>Ōmura, S.</creatorcontrib><title>Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The antibiotic cerulenin inhibited the incorporation of
14C-acetyl-CoA by 67% at a concentration of 9 × 10
−6 M but not that of
14C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of
Saccharomyces cerevisiae. Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity.</description><subject>Acetoacetates</subject><subject>Acetyl Coenzyme A - metabolism</subject><subject>Acetyltransferases - metabolism</subject><subject>Antifungal Agents - pharmacology</subject><subject>Carbon Radioisotopes</subject><subject>Cell-Free System</subject><subject>Coenzyme A</subject><subject>Ethers, Cyclic - pharmacology</subject><subject>Fatty Acids, Unsaturated - pharmacology</subject><subject>Glutarates</subject><subject>Mevalonic Acid - metabolism</subject><subject>Oxo-Acid-Lyases - antagonists & inhibitors</subject><subject>Phytosterols - biosynthesis</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Time Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtrGzEURkVpSJ3HP2jprEq6mPTqMTPSJlBC2gQCWdSBLAJCo7ljq4xHriQH_O-jsY2XXelxz_dxOYR8pnBNgdY_AKAumaIvV434rkBSVrIPZEZBQckoiI9kdkQ-kbMY_wJQKmp1Sk5FpSTn1Yy8zk1YYCp8X7hx6VqXnB-LdlukJRZmTK4c3NovcHR292ydT_lqMWyG_DlOwZgw-KGI2zGHoou5qdiiiemCnPRmiHh5OM_J86-7-e19-fj0--H252NpuaSplK3hKNuqs9BIJWTLVWUt4whcMUTaVNgI2YuaG6Uko7LpGqgbxgQTwK3h5-Tbvncd_L8NxqRXLlocBjOi30QtGc8FgmVQ7EEbfIwBe70ObmXCVlPQk1Q9GdOTMd0IvZOqp9iXQ_-mXWF3DB0s5vnX_bw3XptFcFE__2FAOXAqZK1UJm72BGYNbw6DjtbhaLFzAW3SnXf_X-EduFyPcA</recordid><startdate>19740423</startdate><enddate>19740423</enddate><creator>Ohno, T.</creator><creator>Kesado, T.</creator><creator>Awaya, J.</creator><creator>Ōmura, S.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19740423</creationdate><title>Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast</title><author>Ohno, T. ; Kesado, T. ; Awaya, J. ; Ōmura, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-8ba3e8b5dc078948b395cc23e0392ee175e748f463a9982187d70672242403ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Acetoacetates</topic><topic>Acetyl Coenzyme A - metabolism</topic><topic>Acetyltransferases - metabolism</topic><topic>Antifungal Agents - pharmacology</topic><topic>Carbon Radioisotopes</topic><topic>Cell-Free System</topic><topic>Coenzyme A</topic><topic>Ethers, Cyclic - pharmacology</topic><topic>Fatty Acids, Unsaturated - pharmacology</topic><topic>Glutarates</topic><topic>Mevalonic Acid - metabolism</topic><topic>Oxo-Acid-Lyases - antagonists & inhibitors</topic><topic>Phytosterols - biosynthesis</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohno, T.</creatorcontrib><creatorcontrib>Kesado, T.</creatorcontrib><creatorcontrib>Awaya, J.</creatorcontrib><creatorcontrib>Ōmura, S.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohno, T.</au><au>Kesado, T.</au><au>Awaya, J.</au><au>Ōmura, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1974-04-23</date><risdate>1974</risdate><volume>57</volume><issue>4</issue><spage>1119</spage><epage>1124</epage><pages>1119-1124</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The antibiotic cerulenin inhibited the incorporation of
14C-acetyl-CoA by 67% at a concentration of 9 × 10
−6 M but not that of
14C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of
Saccharomyces cerevisiae. Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4598335</pmid><doi>10.1016/0006-291X(74)90812-2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1974-04, Vol.57 (4), p.1119-1124 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82374842 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetoacetates Acetyl Coenzyme A - metabolism Acetyltransferases - metabolism Antifungal Agents - pharmacology Carbon Radioisotopes Cell-Free System Coenzyme A Ethers, Cyclic - pharmacology Fatty Acids, Unsaturated - pharmacology Glutarates Mevalonic Acid - metabolism Oxo-Acid-Lyases - antagonists & inhibitors Phytosterols - biosynthesis plant biochemistry plant physiology Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Spectrophotometry, Ultraviolet Time Factors |
| title | Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast |
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