A temperature-sensitive hamster cell mutant with altered morphology
A hamster cell mutant, ts-542, was isolated after nitrosoguanidine treatment. The mutant grows well at 33 °C and displays a normal, fibroblast-like morphology. It ceases cell division and becomes web-like in morphology after 4 days at 39 °C. No major lesion occurs in nucleic acid or protein synthesi...
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| Veröffentlicht in: | Experimental cell research 1974-03, Vol.84 (1), p.357-362 |
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| container_title | Experimental cell research |
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| creator | Wang, R.J. Sheridan, W.F. |
| description | A hamster cell mutant, ts-542, was isolated after nitrosoguanidine treatment. The mutant grows well at 33 °C and displays a normal, fibroblast-like morphology. It ceases cell division and becomes web-like in morphology after 4 days at 39 °C. No major lesion occurs in nucleic acid or protein synthesis during the first 3 days following transfer to 39 °C, but by the 4th day at this temperature there is a four-fold increase in acid phosphatase activity, an increase in nucleolar number, nuclear blebbing, and frequency of binucleate cells as well as increase in lysosomes. Cell death at 39 °C is independent of the cell density in the dishes or time of subculture. The mutation appears to be stable with a reversion frequency on the order of 10
−6. |
| doi_str_mv | 10.1016/0014-4827(74)90415-7 |
| format | Article |
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−6.</description><subject>Acid Phosphatase - analysis</subject><subject>Animals</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cell Membrane</subject><subject>Cell Nucleolus</subject><subject>Clone Cells</subject><subject>Cricetinae</subject><subject>Cytoplasm</subject><subject>DNA - biosynthesis</subject><subject>Leucine - metabolism</subject><subject>Lysosomes</subject><subject>Microscopy, Electron</subject><subject>Mutation</subject><subject>Phenotype</subject><subject>Protein Biosynthesis</subject><subject>RNA - biosynthesis</subject><subject>Temperature</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><subject>Uridine - metabolism</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1Lw0AQhhdRaq3-A4WcRA_R2Y_sJhehFL-g4EXPy3YzsStJU3c3lf57E1t69DQw7zvvzDyEXFK4o0DlPQAVqciZulHitgBBs1QdkTGFAlImGDsm44PllJyF8AUAeU7liIxEnknO5ZjMpknEZo3exM5jGnAVXHQbTJamCRF9YrGuk6aLZhWTHxeXian7NpZJ0_r1sq3bz-05OalMHfBiXyfk4-nxffaSzt-eX2fTeWp5pmKaSaxy4AoVLaStoDILKBgyUTDRX4ViUcnciizji5IDAjNoDKPWqMLaSho-Ide73LVvvzsMUTcuDPeZFbZd0DnjTEnFe6PYGa1vQ_BY6bV3jfFbTUEP7PQARg9gtBL6j51W_djVPr9bNFgehvawev1hp2P_5Mah18E6XFksnUcbddm6_xf8AovaflA</recordid><startdate>19740315</startdate><enddate>19740315</enddate><creator>Wang, R.J.</creator><creator>Sheridan, W.F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19740315</creationdate><title>A temperature-sensitive hamster cell mutant with altered morphology</title><author>Wang, R.J. ; Sheridan, W.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-56ef8037e7196cf0fab092e24924816e4bf68c4553bd30e02aeaa21ca79ccf6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Acid Phosphatase - analysis</topic><topic>Animals</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Cell Membrane</topic><topic>Cell Nucleolus</topic><topic>Clone Cells</topic><topic>Cricetinae</topic><topic>Cytoplasm</topic><topic>DNA - biosynthesis</topic><topic>Leucine - metabolism</topic><topic>Lysosomes</topic><topic>Microscopy, Electron</topic><topic>Mutation</topic><topic>Phenotype</topic><topic>Protein Biosynthesis</topic><topic>RNA - biosynthesis</topic><topic>Temperature</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><topic>Uridine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, R.J.</creatorcontrib><creatorcontrib>Sheridan, W.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, R.J.</au><au>Sheridan, W.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A temperature-sensitive hamster cell mutant with altered morphology</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1974-03-15</date><risdate>1974</risdate><volume>84</volume><issue>1</issue><spage>357</spage><epage>362</epage><pages>357-362</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>A hamster cell mutant, ts-542, was isolated after nitrosoguanidine treatment. The mutant grows well at 33 °C and displays a normal, fibroblast-like morphology. It ceases cell division and becomes web-like in morphology after 4 days at 39 °C. No major lesion occurs in nucleic acid or protein synthesis during the first 3 days following transfer to 39 °C, but by the 4th day at this temperature there is a four-fold increase in acid phosphatase activity, an increase in nucleolar number, nuclear blebbing, and frequency of binucleate cells as well as increase in lysosomes. Cell death at 39 °C is independent of the cell density in the dishes or time of subculture. The mutation appears to be stable with a reversion frequency on the order of 10
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| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1974-03, Vol.84 (1), p.357-362 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acid Phosphatase - analysis Animals Cell Division Cell Line Cell Membrane Cell Nucleolus Clone Cells Cricetinae Cytoplasm DNA - biosynthesis Leucine - metabolism Lysosomes Microscopy, Electron Mutation Phenotype Protein Biosynthesis RNA - biosynthesis Temperature Thymidine - metabolism Tritium Uridine - metabolism |
| title | A temperature-sensitive hamster cell mutant with altered morphology |
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