Studies on Clonorchis sinensis. II. Development of an antigen for complement fixation and studies on the antibody response in infected rabbits
An antigen was prepared from adult C. sinensis by treating dehydrated worms with anhydrous ether at −18 °C before extracting with buffered saline solution. Preliminary ether extraction eliminated anticomplementary activity and reduced cross reactivity without effecting the capacity to react specific...
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Veröffentlicht in: | Experimental parasitology 1959-02, Vol.8 (1), p.51-57 |
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creator | Wykoff, Dale E. |
description | An antigen was prepared from adult
C. sinensis by treating dehydrated worms with anhydrous ether at −18 °C before extracting with buffered saline solution. Preliminary ether extraction eliminated anticomplementary activity and reduced cross reactivity without effecting the capacity to react specifically with homologous antiserum. Employing the new antigen in studying the specific antibody response in experimentally infected rabbits, it was shown that it may be used with success to determine host antibody response. |
doi_str_mv | 10.1016/0014-4894(59)90007-4 |
format | Article |
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C. sinensis by treating dehydrated worms with anhydrous ether at −18 °C before extracting with buffered saline solution. Preliminary ether extraction eliminated anticomplementary activity and reduced cross reactivity without effecting the capacity to react specifically with homologous antiserum. Employing the new antigen in studying the specific antibody response in experimentally infected rabbits, it was shown that it may be used with success to determine host antibody response.</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Antigens</subject><subject>Clonorchiasis - immunology</subject><subject>Clonorchis sinensis</subject><subject>Complement System Proteins</subject><subject>Old Medline</subject><subject>Rabbits</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1959</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoMo7uzqPxDJSfTQY6U73T25CDK6OrDgQT2HfFS7ke6kTWUW90_4m-35wL0JBXWo532LeouxFwLWAkT3FkDISm6UfN2qNwoA-ko-YisBCqpaSvWYrf4hF-yS6OfCbEQtn7IL0XSNUq1asT9fy94HJJ4i344ppuxuA3EKESMFWvPdbs0_4B2OaZ4wFp4GbuJSJfzAyIeUuUvTPOJxOITfpoR0mHtOD87lFo8Sm_w9z0hzioQ8xKUGdAU9z8baUOgZezKYkfD5uV-x79cfv20_VzdfPu22728qV_dtqYypobeN8L21zoHpwBrVg7RStmYQshbe-I1wvandoBD63oIy2CrhoG0b1VyxVyffOadfe6Sip0AOx9FETHvSm7qpOyG6BZQn0OVElHHQcw6TyfdagD78QR9C1oeQdav08Q9aLrKXZ_-9ndA_iM7BL8C7E4DLlXcBsyYXMDr0IS-JaJ_C_zf8BWLYmbA</recordid><startdate>195902</startdate><enddate>195902</enddate><creator>Wykoff, Dale E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>195902</creationdate><title>Studies on Clonorchis sinensis. II. Development of an antigen for complement fixation and studies on the antibody response in infected rabbits</title><author>Wykoff, Dale E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-aa207b31d7bbcc0a60ba9704b445af1421dad81c7a2cf9e077b09ae591c055393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1959</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Antigens</topic><topic>Clonorchiasis - immunology</topic><topic>Clonorchis sinensis</topic><topic>Complement System Proteins</topic><topic>Old Medline</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wykoff, Dale E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wykoff, Dale E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on Clonorchis sinensis. II. Development of an antigen for complement fixation and studies on the antibody response in infected rabbits</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>1959-02</date><risdate>1959</risdate><volume>8</volume><issue>1</issue><spage>51</spage><epage>57</epage><pages>51-57</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><abstract>An antigen was prepared from adult
C. sinensis by treating dehydrated worms with anhydrous ether at −18 °C before extracting with buffered saline solution. Preliminary ether extraction eliminated anticomplementary activity and reduced cross reactivity without effecting the capacity to react specifically with homologous antiserum. Employing the new antigen in studying the specific antibody response in experimentally infected rabbits, it was shown that it may be used with success to determine host antibody response.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>13639959</pmid><doi>10.1016/0014-4894(59)90007-4</doi><tpages>7</tpages></addata></record> |
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source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | Animals Antibody Formation Antigens Clonorchiasis - immunology Clonorchis sinensis Complement System Proteins Old Medline Rabbits |
title | Studies on Clonorchis sinensis. II. Development of an antigen for complement fixation and studies on the antibody response in infected rabbits |
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