An improved murine leukemia virus immunofluorescence assay
The use of DEAE-dextran and polybrene during infection of mouse embryo fibroblast cell lines of Fv-1 bb origin with the radiation leukemia virus strikingly increased the proportion of cells in which virus-induced cytoplasmic antigens were detectable by indirect immunofluorescence and significantly a...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1974-02, Vol.57 (2), p.491-502 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 57 |
| creator | Declève, A. Niwa, O. Hilgers, J. Kaplan, H.S. |
| description | The use of DEAE-dextran and polybrene during infection of mouse embryo fibroblast cell lines of Fv-1
bb origin with the radiation leukemia virus strikingly increased the proportion of cells in which virus-induced cytoplasmic antigens were detectable by indirect immunofluorescence and significantly accelerated the time course of their detection, thus making possible the development of a rapid, sensitive, and practical new assay based upon the percentage of immunofluorescence-positive cells. Direct comparisons with several other
in vitro assays are presented. The assay was also tested successfully with Gross leukemia virus grown on cells of Fv-1
nn origin, and should be applicable to other murine leukemia viruses and, perhaps with minor modifications, to other mammalian leukemia viruses as well. |
| doi_str_mv | 10.1016/0042-6822(74)90188-3 |
| format | Article |
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bb origin with the radiation leukemia virus strikingly increased the proportion of cells in which virus-induced cytoplasmic antigens were detectable by indirect immunofluorescence and significantly accelerated the time course of their detection, thus making possible the development of a rapid, sensitive, and practical new assay based upon the percentage of immunofluorescence-positive cells. Direct comparisons with several other
in vitro assays are presented. The assay was also tested successfully with Gross leukemia virus grown on cells of Fv-1
nn origin, and should be applicable to other murine leukemia viruses and, perhaps with minor modifications, to other mammalian leukemia viruses as well.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(74)90188-3</identifier><identifier>PMID: 4361458</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>AKR murine leukemia virus - isolation & purification ; Animals ; Antigens, Viral - analysis ; Cell Line ; Dextrans - pharmacology ; Dimethylamines - pharmacology ; Evaluation Studies as Topic ; Fibroblasts ; Fluorescent Antibody Technique ; Hydrocarbons, Brominated - pharmacology ; Leukemia Virus, Murine - growth & development ; Leukemia Virus, Murine - immunology ; Leukemia Virus, Murine - isolation & purification ; Methods ; Mice ; Microscopy, Fluorescence ; Polyamines - pharmacology ; Polymers - pharmacology ; Time Factors ; Viral Plaque Assay</subject><ispartof>Virology (New York, N.Y.), 1974-02, Vol.57 (2), p.491-502</ispartof><rights>1974</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-f7372884ee62baa7406d7ff915ac2bc96f1c37089b8cc2b1e6bee2f0b5adb6c23</citedby><cites>FETCH-LOGICAL-c357t-f7372884ee62baa7406d7ff915ac2bc96f1c37089b8cc2b1e6bee2f0b5adb6c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0042682274901883$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4361458$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Declève, A.</creatorcontrib><creatorcontrib>Niwa, O.</creatorcontrib><creatorcontrib>Hilgers, J.</creatorcontrib><creatorcontrib>Kaplan, H.S.</creatorcontrib><title>An improved murine leukemia virus immunofluorescence assay</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The use of DEAE-dextran and polybrene during infection of mouse embryo fibroblast cell lines of Fv-1
bb origin with the radiation leukemia virus strikingly increased the proportion of cells in which virus-induced cytoplasmic antigens were detectable by indirect immunofluorescence and significantly accelerated the time course of their detection, thus making possible the development of a rapid, sensitive, and practical new assay based upon the percentage of immunofluorescence-positive cells. Direct comparisons with several other
in vitro assays are presented. The assay was also tested successfully with Gross leukemia virus grown on cells of Fv-1
nn origin, and should be applicable to other murine leukemia viruses and, perhaps with minor modifications, to other mammalian leukemia viruses as well.</description><subject>AKR murine leukemia virus - isolation & purification</subject><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Cell Line</subject><subject>Dextrans - pharmacology</subject><subject>Dimethylamines - pharmacology</subject><subject>Evaluation Studies as Topic</subject><subject>Fibroblasts</subject><subject>Fluorescent Antibody Technique</subject><subject>Hydrocarbons, Brominated - pharmacology</subject><subject>Leukemia Virus, Murine - growth & development</subject><subject>Leukemia Virus, Murine - immunology</subject><subject>Leukemia Virus, Murine - isolation & purification</subject><subject>Methods</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>Polyamines - pharmacology</subject><subject>Polymers - pharmacology</subject><subject>Time Factors</subject><subject>Viral Plaque Assay</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotVbfQGFWoovR3CbJuBBK8QYFN7oOmcwZiM6lJk2hb2_Gli5dHc75_3P7ELok-I5gIu4x5jQXitIbyW9LTJTK2RGaElyKHDNOjtH0YDlFZyF84ZRLiSdowpkgvFBT9DDvM9et_LCBOuuidz1kLcRv6JzJNs7HkOQu9kPTxsFDsNBbyEwIZnuOThrTBrjYxxn6fH76WLzmy_eXt8V8mVtWyHXeSCapUhxA0MoYybGoZdOUpDCWVrYUDbFMYlVWyqYCAVEB0AZXhakrYSmboevd3HTlT4Sw1p1Ld7St6WGIQSvK0vOSJSPfGa0fQvDQ6JV3nfFbTbAekemRhx55aMn1HzI9tl3t58eqg_rQtGeU9MedDunJjQOvg3Ujhtp5sGtdD-7_Bb_Lgnt_</recordid><startdate>197402</startdate><enddate>197402</enddate><creator>Declève, A.</creator><creator>Niwa, O.</creator><creator>Hilgers, J.</creator><creator>Kaplan, H.S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197402</creationdate><title>An improved murine leukemia virus immunofluorescence assay</title><author>Declève, A. ; Niwa, O. ; Hilgers, J. ; Kaplan, H.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-f7372884ee62baa7406d7ff915ac2bc96f1c37089b8cc2b1e6bee2f0b5adb6c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>AKR murine leukemia virus - isolation & purification</topic><topic>Animals</topic><topic>Antigens, Viral - analysis</topic><topic>Cell Line</topic><topic>Dextrans - pharmacology</topic><topic>Dimethylamines - pharmacology</topic><topic>Evaluation Studies as Topic</topic><topic>Fibroblasts</topic><topic>Fluorescent Antibody Technique</topic><topic>Hydrocarbons, Brominated - pharmacology</topic><topic>Leukemia Virus, Murine - growth & development</topic><topic>Leukemia Virus, Murine - immunology</topic><topic>Leukemia Virus, Murine - isolation & purification</topic><topic>Methods</topic><topic>Mice</topic><topic>Microscopy, Fluorescence</topic><topic>Polyamines - pharmacology</topic><topic>Polymers - pharmacology</topic><topic>Time Factors</topic><topic>Viral Plaque Assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Declève, A.</creatorcontrib><creatorcontrib>Niwa, O.</creatorcontrib><creatorcontrib>Hilgers, J.</creatorcontrib><creatorcontrib>Kaplan, H.S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Declève, A.</au><au>Niwa, O.</au><au>Hilgers, J.</au><au>Kaplan, H.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved murine leukemia virus immunofluorescence assay</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1974-02</date><risdate>1974</risdate><volume>57</volume><issue>2</issue><spage>491</spage><epage>502</epage><pages>491-502</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>The use of DEAE-dextran and polybrene during infection of mouse embryo fibroblast cell lines of Fv-1
bb origin with the radiation leukemia virus strikingly increased the proportion of cells in which virus-induced cytoplasmic antigens were detectable by indirect immunofluorescence and significantly accelerated the time course of their detection, thus making possible the development of a rapid, sensitive, and practical new assay based upon the percentage of immunofluorescence-positive cells. Direct comparisons with several other
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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1974-02, Vol.57 (2), p.491-502 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82301873 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | AKR murine leukemia virus - isolation & purification Animals Antigens, Viral - analysis Cell Line Dextrans - pharmacology Dimethylamines - pharmacology Evaluation Studies as Topic Fibroblasts Fluorescent Antibody Technique Hydrocarbons, Brominated - pharmacology Leukemia Virus, Murine - growth & development Leukemia Virus, Murine - immunology Leukemia Virus, Murine - isolation & purification Methods Mice Microscopy, Fluorescence Polyamines - pharmacology Polymers - pharmacology Time Factors Viral Plaque Assay |
| title | An improved murine leukemia virus immunofluorescence assay |
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