Characterization and properties of an anaphylactic 7S antibody in sheep
Anti-DNP antibody was specifically purified from hyperimmune anti-DNP-BGG sheep antiserum. The antibody was fractionated on DEAE-cellulose. Two components belonging to different immunoglobulin classes were isolated and designated IgS (slow) and IgF (fast) on account of their relative electrophoretic...
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| Veröffentlicht in: | The Journal of immunology (1950) 1974-02, Vol.112 (2), p.722-727 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 112 |
| creator | Esteves, M.B Sant'Anna, O.A Santos-Annes, V.C. dos Binaghi, R.A |
| description | Anti-DNP antibody was specifically purified from hyperimmune anti-DNP-BGG sheep antiserum. The antibody was fractionated on DEAE-cellulose. Two components belonging to different immunoglobulin classes were isolated and designated IgS (slow) and IgF (fast) on account of their relative electrophoretic mobility. The molecular size of both antibodies is the same and it is similar to that of human IgG. IgS induces homologous anaphylactic reactions (PCA) and does not fix complement in the classical way. IgF fixes complement and is not anaphylactic for the sheep. The biologic activities of IgS are not destroyed by treatment with 2-mercaptoethanol. The skin sensitization obtained with IgS does not persist more than 2 hr. |
| doi_str_mv | 10.4049/jimmunol.112.2.722 |
| format | Article |
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The antibody was fractionated on DEAE-cellulose. Two components belonging to different immunoglobulin classes were isolated and designated IgS (slow) and IgF (fast) on account of their relative electrophoretic mobility. The molecular size of both antibodies is the same and it is similar to that of human IgG. IgS induces homologous anaphylactic reactions (PCA) and does not fix complement in the classical way. IgF fixes complement and is not anaphylactic for the sheep. The biologic activities of IgS are not destroyed by treatment with 2-mercaptoethanol. The skin sensitization obtained with IgS does not persist more than 2 hr.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.112.2.722</identifier><identifier>PMID: 4130695</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Anaphylaxis ; animal diseases ; animal health ; Animals ; Antibodies - analysis ; Antibodies - isolation & purification ; Arthus Reaction ; Cattle - immunology ; Chromatography, DEAE-Cellulose ; Complement Fixation Tests ; Dinitrophenols ; gamma-Globulins ; Guinea Pigs - immunology ; Hemagglutination Tests ; Hemolysis ; Humans ; Immune Sera ; Immunoelectrophoresis ; Immunoglobulin G - analysis ; Molecular Weight ; Passive Cutaneous Anaphylaxis ; Rabbits - immunology ; Sheep - immunology</subject><ispartof>The Journal of immunology (1950), 1974-02, Vol.112 (2), p.722-727</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-ed162c0fb61c7b53e4e260933edb77f6849eb47c0b061ae5e8bb8961eed82d323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4130695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Esteves, M.B</creatorcontrib><creatorcontrib>Sant'Anna, O.A</creatorcontrib><creatorcontrib>Santos-Annes, V.C. dos</creatorcontrib><creatorcontrib>Binaghi, R.A</creatorcontrib><title>Characterization and properties of an anaphylactic 7S antibody in sheep</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Anti-DNP antibody was specifically purified from hyperimmune anti-DNP-BGG sheep antiserum. The antibody was fractionated on DEAE-cellulose. Two components belonging to different immunoglobulin classes were isolated and designated IgS (slow) and IgF (fast) on account of their relative electrophoretic mobility. The molecular size of both antibodies is the same and it is similar to that of human IgG. IgS induces homologous anaphylactic reactions (PCA) and does not fix complement in the classical way. IgF fixes complement and is not anaphylactic for the sheep. The biologic activities of IgS are not destroyed by treatment with 2-mercaptoethanol. The skin sensitization obtained with IgS does not persist more than 2 hr.</description><subject>Anaphylaxis</subject><subject>animal diseases</subject><subject>animal health</subject><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibodies - isolation & purification</subject><subject>Arthus Reaction</subject><subject>Cattle - immunology</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Complement Fixation Tests</subject><subject>Dinitrophenols</subject><subject>gamma-Globulins</subject><subject>Guinea Pigs - immunology</subject><subject>Hemagglutination Tests</subject><subject>Hemolysis</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Immunoelectrophoresis</subject><subject>Immunoglobulin G - analysis</subject><subject>Molecular Weight</subject><subject>Passive Cutaneous Anaphylaxis</subject><subject>Rabbits - immunology</subject><subject>Sheep - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkF1LwzAUQIMoOqd_QBD75FvnTdIm7aMMncLAB_U5JO2tjfTLpKPMX29k8wMCIeHck3AIuaCwSCDJb95t2266vllQyhZsIRk7IDOaphALAeKQzAAYi6kU8oScev8OAAJYckyOE8pB5OmMrJa1droY0dlPPdq-i3RXRoPrB3SjRR_1VbgJSw_1tgmgLSL5HM6jNX25jWwX-RpxOCNHlW48nu_3OXm9v3tZPsTrp9Xj8nYdFzyXY4wlFayAyghaSJNyTJAJyDnH0khZiSzJ0SSyAAOCakwxMybLBUUsM1ZyxufkeucNX_zYoB9Va32BTaM77DdeZYwDTWQeQLYDC9d777BSg7OtdltFQX3XUz_1VKinmAr1wtDl3r4xLZa_I_tcf6_X9q2erEPlW900gaZqmqb_oqsdWOle6TdnvXp9ZhA0nLJgkvwLM4OEBg</recordid><startdate>197402</startdate><enddate>197402</enddate><creator>Esteves, M.B</creator><creator>Sant'Anna, O.A</creator><creator>Santos-Annes, V.C. dos</creator><creator>Binaghi, R.A</creator><general>Am Assoc Immnol</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197402</creationdate><title>Characterization and properties of an anaphylactic 7S antibody in sheep</title><author>Esteves, M.B ; Sant'Anna, O.A ; Santos-Annes, V.C. dos ; Binaghi, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-ed162c0fb61c7b53e4e260933edb77f6849eb47c0b061ae5e8bb8961eed82d323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Anaphylaxis</topic><topic>animal diseases</topic><topic>animal health</topic><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>Antibodies - isolation & purification</topic><topic>Arthus Reaction</topic><topic>Cattle - immunology</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Complement Fixation Tests</topic><topic>Dinitrophenols</topic><topic>gamma-Globulins</topic><topic>Guinea Pigs - immunology</topic><topic>Hemagglutination Tests</topic><topic>Hemolysis</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Immunoelectrophoresis</topic><topic>Immunoglobulin G - analysis</topic><topic>Molecular Weight</topic><topic>Passive Cutaneous Anaphylaxis</topic><topic>Rabbits - immunology</topic><topic>Sheep - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Esteves, M.B</creatorcontrib><creatorcontrib>Sant'Anna, O.A</creatorcontrib><creatorcontrib>Santos-Annes, V.C. dos</creatorcontrib><creatorcontrib>Binaghi, R.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Esteves, M.B</au><au>Sant'Anna, O.A</au><au>Santos-Annes, V.C. dos</au><au>Binaghi, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and properties of an anaphylactic 7S antibody in sheep</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1974-02</date><risdate>1974</risdate><volume>112</volume><issue>2</issue><spage>722</spage><epage>727</epage><pages>722-727</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Anti-DNP antibody was specifically purified from hyperimmune anti-DNP-BGG sheep antiserum. The antibody was fractionated on DEAE-cellulose. Two components belonging to different immunoglobulin classes were isolated and designated IgS (slow) and IgF (fast) on account of their relative electrophoretic mobility. The molecular size of both antibodies is the same and it is similar to that of human IgG. IgS induces homologous anaphylactic reactions (PCA) and does not fix complement in the classical way. IgF fixes complement and is not anaphylactic for the sheep. The biologic activities of IgS are not destroyed by treatment with 2-mercaptoethanol. The skin sensitization obtained with IgS does not persist more than 2 hr.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>4130695</pmid><doi>10.4049/jimmunol.112.2.722</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 1974-02, Vol.112 (2), p.722-727 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Anaphylaxis animal diseases animal health Animals Antibodies - analysis Antibodies - isolation & purification Arthus Reaction Cattle - immunology Chromatography, DEAE-Cellulose Complement Fixation Tests Dinitrophenols gamma-Globulins Guinea Pigs - immunology Hemagglutination Tests Hemolysis Humans Immune Sera Immunoelectrophoresis Immunoglobulin G - analysis Molecular Weight Passive Cutaneous Anaphylaxis Rabbits - immunology Sheep - immunology |
| title | Characterization and properties of an anaphylactic 7S antibody in sheep |
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