Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy
Summary Background Overproduction of collagen and its abnormal assembly are hallmarks of keloid scars. Type I/III collagen ratios are altered in keloids compared with normal skin. Fibroblasts from different sites in keloid tissue, perilesional compared with intralesional and extralesional sites, sh...
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| Veröffentlicht in: | British journal of dermatology (1951) 2011-01, Vol.164 (1), p.83-96 |
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| container_title | British journal of dermatology (1951) |
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| creator | Syed, F. Ahmadi, E. Iqbal, S.A. Singh, S. McGrouther, D.A. Bayat, A. |
| description | Summary
Background Overproduction of collagen and its abnormal assembly are hallmarks of keloid scars. Type I/III collagen ratios are altered in keloids compared with normal skin. Fibroblasts from different sites in keloid tissue, perilesional compared with intralesional and extralesional sites, show differential apoptosis and contraction. Additionally, early vs. later cell culture passages display differential collagen expression. We therefore hypothesize that keloid fibroblasts from the growing margin of the keloid express higher levels of collagen type I and III, and that collagen production is altered by extended cell culture passage.
Objectives (i) To measure collagen I and III at mRNA and protein levels quantitatively in keloid fibroblasts, growth media and tissue sections; and (ii) to perform tissue staining for collagen I and III expression in different lesional sites.
Methods Keloid fibroblast cultures from intralesional, perilesional and extralesional sites (n = 8 separate keloid cases, yielding 64 biopsy samples) were established from passage 0 to passage 3. Collagen I and III mRNA was quantified using quantitative reverse transcription–polymerase chain reaction. We also measured the protein levels quantitatively by developing a highly specific and sensitive capture sandwich enzyme‐linked immunosorbent assay. A novel in‐cell Western blotting was carried out in addition to haematoxylin and eosin and Herovici staining on keloid tissue sections for collagen I and III.
Results Collagen types I and III were significantly higher (P |
| doi_str_mv | 10.1111/j.1365-2133.2010.10048.x |
| format | Article |
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Background Overproduction of collagen and its abnormal assembly are hallmarks of keloid scars. Type I/III collagen ratios are altered in keloids compared with normal skin. Fibroblasts from different sites in keloid tissue, perilesional compared with intralesional and extralesional sites, show differential apoptosis and contraction. Additionally, early vs. later cell culture passages display differential collagen expression. We therefore hypothesize that keloid fibroblasts from the growing margin of the keloid express higher levels of collagen type I and III, and that collagen production is altered by extended cell culture passage.
Objectives (i) To measure collagen I and III at mRNA and protein levels quantitatively in keloid fibroblasts, growth media and tissue sections; and (ii) to perform tissue staining for collagen I and III expression in different lesional sites.
Methods Keloid fibroblast cultures from intralesional, perilesional and extralesional sites (n = 8 separate keloid cases, yielding 64 biopsy samples) were established from passage 0 to passage 3. Collagen I and III mRNA was quantified using quantitative reverse transcription–polymerase chain reaction. We also measured the protein levels quantitatively by developing a highly specific and sensitive capture sandwich enzyme‐linked immunosorbent assay. A novel in‐cell Western blotting was carried out in addition to haematoxylin and eosin and Herovici staining on keloid tissue sections for collagen I and III.
Results Collagen types I and III were significantly higher (P < 0·03) in fibroblasts from the growing margin (perilesional site) compared with extralesional and intralesional keloid biopsy sites. As the passage number increased, the amount of collagen I significantly (P < 0·05) decreased and the collagen I/III ratio also decreased.
Conclusions Our data show that cells from the growing margin of keloid scars have a higher production of collagen I and III compared with other lesional sites. Additionally, temporal extension of cell passage affects collagen production. Clinically these findings may influence selection and interpretation of extended cell passage and provide future direction for lesional site‐specific therapy in keloid scars.</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1111/j.1365-2133.2010.10048.x</identifier><identifier>PMID: 20849516</identifier><identifier>CODEN: BJDEAZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Biopsy ; Blotting, Western ; Collagen Type I - biosynthesis ; Collagen Type III - biosynthesis ; Dermatology ; Female ; Fibroblasts - metabolism ; Humans ; Keloid - pathology ; Male ; Medical sciences ; Polymerase Chain Reaction - methods ; RNA, Messenger - metabolism ; Skin - metabolism ; Skin - pathology ; Skin involvement in other diseases. Miscellaneous. General aspects ; Young Adult</subject><ispartof>British journal of dermatology (1951), 2011-01, Vol.164 (1), p.83-96</ispartof><rights>2011 The Authors. BJD © 2011 British Association of Dermatologists 2011</rights><rights>2015 INIST-CNRS</rights><rights>2011 The Authors. BJD © 2011 British Association of Dermatologists 2011.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3888-68a995d1be6789aa867924e34cd7ad5a8373a284e2e421bb3721a2dd9b165b8f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2133.2010.10048.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2133.2010.10048.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,4010,27904,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23725250$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20849516$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Syed, F.</creatorcontrib><creatorcontrib>Ahmadi, E.</creatorcontrib><creatorcontrib>Iqbal, S.A.</creatorcontrib><creatorcontrib>Singh, S.</creatorcontrib><creatorcontrib>McGrouther, D.A.</creatorcontrib><creatorcontrib>Bayat, A.</creatorcontrib><title>Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy</title><title>British journal of dermatology (1951)</title><addtitle>Br J Dermatol</addtitle><description>Summary
Background Overproduction of collagen and its abnormal assembly are hallmarks of keloid scars. Type I/III collagen ratios are altered in keloids compared with normal skin. Fibroblasts from different sites in keloid tissue, perilesional compared with intralesional and extralesional sites, show differential apoptosis and contraction. Additionally, early vs. later cell culture passages display differential collagen expression. We therefore hypothesize that keloid fibroblasts from the growing margin of the keloid express higher levels of collagen type I and III, and that collagen production is altered by extended cell culture passage.
Objectives (i) To measure collagen I and III at mRNA and protein levels quantitatively in keloid fibroblasts, growth media and tissue sections; and (ii) to perform tissue staining for collagen I and III expression in different lesional sites.
Methods Keloid fibroblast cultures from intralesional, perilesional and extralesional sites (n = 8 separate keloid cases, yielding 64 biopsy samples) were established from passage 0 to passage 3. Collagen I and III mRNA was quantified using quantitative reverse transcription–polymerase chain reaction. We also measured the protein levels quantitatively by developing a highly specific and sensitive capture sandwich enzyme‐linked immunosorbent assay. A novel in‐cell Western blotting was carried out in addition to haematoxylin and eosin and Herovici staining on keloid tissue sections for collagen I and III.
Results Collagen types I and III were significantly higher (P < 0·03) in fibroblasts from the growing margin (perilesional site) compared with extralesional and intralesional keloid biopsy sites. As the passage number increased, the amount of collagen I significantly (P < 0·05) decreased and the collagen I/III ratio also decreased.
Conclusions Our data show that cells from the growing margin of keloid scars have a higher production of collagen I and III compared with other lesional sites. Additionally, temporal extension of cell passage affects collagen production. Clinically these findings may influence selection and interpretation of extended cell passage and provide future direction for lesional site‐specific therapy in keloid scars.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Blotting, Western</subject><subject>Collagen Type I - biosynthesis</subject><subject>Collagen Type III - biosynthesis</subject><subject>Dermatology</subject><subject>Female</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Keloid - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - metabolism</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Skin involvement in other diseases. Miscellaneous. General aspects</subject><subject>Young Adult</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNktuO0zAQhi0EYsvCKyDfIK5SfMjB4QKJXehu0Kogcbq0nHiSuuscsFPaPiTvhNOWsr7xeObTP-OZQQhTMqfhvFnPKU-TiFHO54xMXkJiMd89QrNz4DGaEUKyiOQpv0DPvF8TQjlJyFN0wYiI84SmM_RnYUrXl1b50ePa9S0eV4Ab129N1-BWucZ0uK_xPdjeaOwr5TweXK83FeCVaVbgsIXfYP1EVb21qoEOF1h1GhdFEVztoBxovDXjCptudMqCN32n7IGB3UOPNyP4t7iypjNVeJt2sMEYQzSU10-5HpCRNg6qMYiHop0a9s_Rk1pZDy9O9yX6vvj47fo2uvt8U1y_v4sqLoSIUqHyPNG0hDQTuVIizXIWA48rnSmdKMEzrpiIgUHMaFnyjFHFtM5LmialqPklen3UDZ34tQE_ytb4CsLnO-g3XgrGckLTmAfy5YnclC1oOTgTmrqX_yYQgFcnQIXm2tqprjL-PxdyJywhgXt35LbGwv4cp0ROGyHXchq8nAYvp42Qh42QO3n16cPBDALRUcD4EXZnAeXuZZrxLJE_lzeSL77cLq--_pBL_hfN8r0b</recordid><startdate>201101</startdate><enddate>201101</enddate><creator>Syed, F.</creator><creator>Ahmadi, E.</creator><creator>Iqbal, S.A.</creator><creator>Singh, S.</creator><creator>McGrouther, D.A.</creator><creator>Bayat, A.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201101</creationdate><title>Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy</title><author>Syed, F. ; Ahmadi, E. ; Iqbal, S.A. ; Singh, S. ; McGrouther, D.A. ; Bayat, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3888-68a995d1be6789aa867924e34cd7ad5a8373a284e2e421bb3721a2dd9b165b8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Blotting, Western</topic><topic>Collagen Type I - biosynthesis</topic><topic>Collagen Type III - biosynthesis</topic><topic>Dermatology</topic><topic>Female</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Keloid - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - metabolism</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><topic>Skin involvement in other diseases. Miscellaneous. General aspects</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Syed, F.</creatorcontrib><creatorcontrib>Ahmadi, E.</creatorcontrib><creatorcontrib>Iqbal, S.A.</creatorcontrib><creatorcontrib>Singh, S.</creatorcontrib><creatorcontrib>McGrouther, D.A.</creatorcontrib><creatorcontrib>Bayat, A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Syed, F.</au><au>Ahmadi, E.</au><au>Iqbal, S.A.</au><au>Singh, S.</au><au>McGrouther, D.A.</au><au>Bayat, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>2011-01</date><risdate>2011</risdate><volume>164</volume><issue>1</issue><spage>83</spage><epage>96</epage><pages>83-96</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><coden>BJDEAZ</coden><abstract>Summary
Background Overproduction of collagen and its abnormal assembly are hallmarks of keloid scars. Type I/III collagen ratios are altered in keloids compared with normal skin. Fibroblasts from different sites in keloid tissue, perilesional compared with intralesional and extralesional sites, show differential apoptosis and contraction. Additionally, early vs. later cell culture passages display differential collagen expression. We therefore hypothesize that keloid fibroblasts from the growing margin of the keloid express higher levels of collagen type I and III, and that collagen production is altered by extended cell culture passage.
Objectives (i) To measure collagen I and III at mRNA and protein levels quantitatively in keloid fibroblasts, growth media and tissue sections; and (ii) to perform tissue staining for collagen I and III expression in different lesional sites.
Methods Keloid fibroblast cultures from intralesional, perilesional and extralesional sites (n = 8 separate keloid cases, yielding 64 biopsy samples) were established from passage 0 to passage 3. Collagen I and III mRNA was quantified using quantitative reverse transcription–polymerase chain reaction. We also measured the protein levels quantitatively by developing a highly specific and sensitive capture sandwich enzyme‐linked immunosorbent assay. A novel in‐cell Western blotting was carried out in addition to haematoxylin and eosin and Herovici staining on keloid tissue sections for collagen I and III.
Results Collagen types I and III were significantly higher (P < 0·03) in fibroblasts from the growing margin (perilesional site) compared with extralesional and intralesional keloid biopsy sites. As the passage number increased, the amount of collagen I significantly (P < 0·05) decreased and the collagen I/III ratio also decreased.
Conclusions Our data show that cells from the growing margin of keloid scars have a higher production of collagen I and III compared with other lesional sites. Additionally, temporal extension of cell passage affects collagen production. Clinically these findings may influence selection and interpretation of extended cell passage and provide future direction for lesional site‐specific therapy in keloid scars.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20849516</pmid><doi>10.1111/j.1365-2133.2010.10048.x</doi><tpages>14</tpages></addata></record> |
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| subjects | Adolescent Adult Biological and medical sciences Biopsy Blotting, Western Collagen Type I - biosynthesis Collagen Type III - biosynthesis Dermatology Female Fibroblasts - metabolism Humans Keloid - pathology Male Medical sciences Polymerase Chain Reaction - methods RNA, Messenger - metabolism Skin - metabolism Skin - pathology Skin involvement in other diseases. Miscellaneous. General aspects Young Adult |
| title | Fibroblasts from the growing margin of keloid scars produce higher levels of collagen I and III compared with intralesional and extralesional sites: clinical implications for lesional site-directed therapy |
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