Opposite effects of sodium butyrate on CCK mRNA and CCK peptide levels in RIN cells

Opposite effects of sodium butyrate on CCK mRNA and CCK peptide levels in RIN cells

The effects of the differentiation-inducing agent sodium butyrate on cholecystokinin (CCK) expression was investigated in the pancreatic islet tumor cell line RIN 1056E, which contains high levels of CCK-like immunoreactivity (CCK-LI). Exposure to butyrate for 24 h dose-dependently inhibited cell pr...

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Veröffentlicht in:Endocrine 1996-12, Vol.5 (3), p.331-334
Hauptverfasser: Roche, C, Cordier-Bussat, M, Ratineau, C, Bernard, C, Philippe, J, Cuber, J C
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container_end_page 334
container_issue 3
container_start_page 331
container_title Endocrine
container_volume 5
creator Roche, C
Cordier-Bussat, M
Ratineau, C
Bernard, C
Philippe, J
Cuber, J C
description The effects of the differentiation-inducing agent sodium butyrate on cholecystokinin (CCK) expression was investigated in the pancreatic islet tumor cell line RIN 1056E, which contains high levels of CCK-like immunoreactivity (CCK-LI). Exposure to butyrate for 24 h dose-dependently inhibited cell proliferation and increased the cell content in CCK-LI over the concentration range 0.1-8 mM. With 2 mM butyrate, cell proliferation was decreased by 50% and CCK-LI content was increased by 300%, whereas the level of steady-state CCK mRNA was reduced by 75%. Cycloheximide (10 μg/mL) abolished the sodium butyrate-induced increase in CCK-LI content. This article reports the novel finding that butyrate exerts opposite effects on CCK mRNA and immunoreactivity. The butyrate-induced increase in cellular CCK-LI content is entirely dependent on continuing protein synthesis.
doi_str_mv 10.1007/BF02739067
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title Opposite effects of sodium butyrate on CCK mRNA and CCK peptide levels in RIN cells
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