RESPONSE IN MACROMOLECULAR SYNTHESES OF MOUSE L CELLS TO BLEOMYCIN, WITH SPECIAL REFERENCE TO CELL-ANTIBIOTIC INTERACTION
The inhibitory effect of bleomycin on DNA, RNA and protein syntheses of mouse L cells was determined. DNA synthesis was most affected. Protein and RNA syntheses were less affected in this order. Inhibition of DNA synthesis exhibited an upward-concave curve as a function of time of exposure to bleomy...
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| Veröffentlicht in: | Journal of antibiotics 1973/08/25, Vol.26(8), pp.417-423 |
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| container_end_page | 423 |
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| container_issue | 8 |
| container_start_page | 417 |
| container_title | Journal of antibiotics |
| container_volume | 26 |
| creator | WATANABE, MICHINORI TAKABE, YOSINOBU KATSUMATA, TAKESI TERASIMA, TOYOZO UMEZAWA, HAMAO |
| description | The inhibitory effect of bleomycin on DNA, RNA and protein syntheses of mouse L cells was determined. DNA synthesis was most affected. Protein and RNA syntheses were less affected in this order. Inhibition of DNA synthesis exhibited an upward-concave curve as a function of time of exposure to bleomycin. Analysis of this particular curvature revealed a characteristic interaction between the antibiotic and mammalian cells. Results obtained from the experiments of two pulsed drug treatments showed that (1) when bleomycin was introduced into culture, cell resistance developed with time and was complete within 60 minutes, and (2) the resistance so induced disappeared in approximately 2 hours upon removal of bleomycin. The nature of this resistance was not elucidated. Either an enzyme which inactivates bleomycin or DNA-repair enzymes may be responsible for resistance. The above interaction may have some relevance to bleomycin chemotherapy. |
| doi_str_mv | 10.7164/antibiotics.26.417 |
| format | Article |
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DNA synthesis was most affected. Protein and RNA syntheses were less affected in this order. Inhibition of DNA synthesis exhibited an upward-concave curve as a function of time of exposure to bleomycin. Analysis of this particular curvature revealed a characteristic interaction between the antibiotic and mammalian cells. Results obtained from the experiments of two pulsed drug treatments showed that (1) when bleomycin was introduced into culture, cell resistance developed with time and was complete within 60 minutes, and (2) the resistance so induced disappeared in approximately 2 hours upon removal of bleomycin. The nature of this resistance was not elucidated. Either an enzyme which inactivates bleomycin or DNA-repair enzymes may be responsible for resistance. The above interaction may have some relevance to bleomycin chemotherapy.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.7164/antibiotics.26.417</identifier><identifier>PMID: 4134981</identifier><language>eng</language><publisher>Japan: JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</publisher><subject>Animals ; Autoradiography ; Bleomycin - pharmacology ; Carbon Radioisotopes ; Depression, Chemical ; DNA - biosynthesis ; DNA Repair ; Drug Resistance ; L Cells (Cell Line) - drug effects ; L Cells (Cell Line) - metabolism ; Leucine - metabolism ; Mice ; Protein Biosynthesis ; RNA - biosynthesis ; Thymidine - metabolism ; Time Factors ; Tritium ; Uridine - metabolism</subject><ispartof>The Journal of Antibiotics, 1973/08/25, Vol.26(8), pp.417-423</ispartof><rights>Japan Antibiotics Research Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-f89f2892b535d9dbdb2424e37041d5d5dd522fb0f5551a4b3607810846672c1f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4134981$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WATANABE, MICHINORI</creatorcontrib><creatorcontrib>TAKABE, YOSINOBU</creatorcontrib><creatorcontrib>KATSUMATA, TAKESI</creatorcontrib><creatorcontrib>TERASIMA, TOYOZO</creatorcontrib><creatorcontrib>UMEZAWA, HAMAO</creatorcontrib><title>RESPONSE IN MACROMOLECULAR SYNTHESES OF MOUSE L CELLS TO BLEOMYCIN, WITH SPECIAL REFERENCE TO CELL-ANTIBIOTIC INTERACTION</title><title>Journal of antibiotics</title><addtitle>J. Antibiot.</addtitle><description>The inhibitory effect of bleomycin on DNA, RNA and protein syntheses of mouse L cells was determined. DNA synthesis was most affected. Protein and RNA syntheses were less affected in this order. Inhibition of DNA synthesis exhibited an upward-concave curve as a function of time of exposure to bleomycin. Analysis of this particular curvature revealed a characteristic interaction between the antibiotic and mammalian cells. Results obtained from the experiments of two pulsed drug treatments showed that (1) when bleomycin was introduced into culture, cell resistance developed with time and was complete within 60 minutes, and (2) the resistance so induced disappeared in approximately 2 hours upon removal of bleomycin. The nature of this resistance was not elucidated. Either an enzyme which inactivates bleomycin or DNA-repair enzymes may be responsible for resistance. The above interaction may have some relevance to bleomycin chemotherapy.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Bleomycin - pharmacology</subject><subject>Carbon Radioisotopes</subject><subject>Depression, Chemical</subject><subject>DNA - biosynthesis</subject><subject>DNA Repair</subject><subject>Drug Resistance</subject><subject>L Cells (Cell Line) - drug effects</subject><subject>L Cells (Cell Line) - metabolism</subject><subject>Leucine - metabolism</subject><subject>Mice</subject><subject>Protein Biosynthesis</subject><subject>RNA - biosynthesis</subject><subject>Thymidine - metabolism</subject><subject>Time Factors</subject><subject>Tritium</subject><subject>Uridine - metabolism</subject><issn>0021-8820</issn><issn>1881-1469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkF1r2zAUhkXZ6LKuf6Aw0NWu5lSSZVm-dIWyiCpWsR1Kr4w_5M0lH63lXPTfTyEhlAM6F-9zHsELwB1G8xgzel_vpqEZ9tPQujlhc4rjKzDDnOMAU5Z8ATOECA44J-gb-O7cK0JhHMb8GlxTHNKE4xn4yGXxZLJCQpXBVSpyszJairVOc1i8ZOVSFrKAZgFXZu0hDYXUuoClgQ9amtWLUNlv-KzKJSyepFCphrlcyFxmQh6hIx2kWakelCmV8J-UMk9FqUz2A3zt642zt-d9A9YLWYploM0fJVIdtDRmU9DzpCc8IU0URl3SNV1DKKE2jBHFXeSniwjpG9RHUYRr2oQMxRwjThmLSYv78Ab8Onnfxv37wbqp2g6utZtNvbP7g6s4ITTENPIgOYHtuHdutH31Ng7bevyoMKqOfVef-q4Iq3zf_ujn2X5otra7nJwL9vnjKX91U_3XXvJ69JaN_azECeNH7fnx9gvV_qvHyu7C_9Qdj6o</recordid><startdate>19730101</startdate><enddate>19730101</enddate><creator>WATANABE, MICHINORI</creator><creator>TAKABE, YOSINOBU</creator><creator>KATSUMATA, TAKESI</creator><creator>TERASIMA, TOYOZO</creator><creator>UMEZAWA, HAMAO</creator><general>JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19730101</creationdate><title>RESPONSE IN MACROMOLECULAR SYNTHESES OF MOUSE L CELLS TO BLEOMYCIN, WITH SPECIAL REFERENCE TO CELL-ANTIBIOTIC INTERACTION</title><author>WATANABE, MICHINORI ; TAKABE, YOSINOBU ; KATSUMATA, TAKESI ; TERASIMA, TOYOZO ; UMEZAWA, HAMAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-f89f2892b535d9dbdb2424e37041d5d5dd522fb0f5551a4b3607810846672c1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Bleomycin - pharmacology</topic><topic>Carbon Radioisotopes</topic><topic>Depression, Chemical</topic><topic>DNA - biosynthesis</topic><topic>DNA Repair</topic><topic>Drug Resistance</topic><topic>L Cells (Cell Line) - drug effects</topic><topic>L Cells (Cell Line) - metabolism</topic><topic>Leucine - metabolism</topic><topic>Mice</topic><topic>Protein Biosynthesis</topic><topic>RNA - biosynthesis</topic><topic>Thymidine - metabolism</topic><topic>Time Factors</topic><topic>Tritium</topic><topic>Uridine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WATANABE, MICHINORI</creatorcontrib><creatorcontrib>TAKABE, YOSINOBU</creatorcontrib><creatorcontrib>KATSUMATA, TAKESI</creatorcontrib><creatorcontrib>TERASIMA, TOYOZO</creatorcontrib><creatorcontrib>UMEZAWA, HAMAO</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antibiotics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WATANABE, MICHINORI</au><au>TAKABE, YOSINOBU</au><au>KATSUMATA, TAKESI</au><au>TERASIMA, TOYOZO</au><au>UMEZAWA, HAMAO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RESPONSE IN MACROMOLECULAR SYNTHESES OF MOUSE L CELLS TO BLEOMYCIN, WITH SPECIAL REFERENCE TO CELL-ANTIBIOTIC INTERACTION</atitle><jtitle>Journal of antibiotics</jtitle><addtitle>J. Antibiot.</addtitle><date>1973-01-01</date><risdate>1973</risdate><volume>26</volume><issue>8</issue><spage>417</spage><epage>423</epage><pages>417-423</pages><issn>0021-8820</issn><eissn>1881-1469</eissn><abstract>The inhibitory effect of bleomycin on DNA, RNA and protein syntheses of mouse L cells was determined. DNA synthesis was most affected. Protein and RNA syntheses were less affected in this order. Inhibition of DNA synthesis exhibited an upward-concave curve as a function of time of exposure to bleomycin. Analysis of this particular curvature revealed a characteristic interaction between the antibiotic and mammalian cells. Results obtained from the experiments of two pulsed drug treatments showed that (1) when bleomycin was introduced into culture, cell resistance developed with time and was complete within 60 minutes, and (2) the resistance so induced disappeared in approximately 2 hours upon removal of bleomycin. The nature of this resistance was not elucidated. Either an enzyme which inactivates bleomycin or DNA-repair enzymes may be responsible for resistance. The above interaction may have some relevance to bleomycin chemotherapy.</abstract><cop>Japan</cop><pub>JAPAN ANTIBIOTICS RESEARCH ASSOCIATION</pub><pmid>4134981</pmid><doi>10.7164/antibiotics.26.417</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8820 |
| ispartof | The Journal of Antibiotics, 1973/08/25, Vol.26(8), pp.417-423 |
| issn | 0021-8820 1881-1469 |
| language | eng |
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| source | J-STAGE Free; MEDLINE |
| subjects | Animals Autoradiography Bleomycin - pharmacology Carbon Radioisotopes Depression, Chemical DNA - biosynthesis DNA Repair Drug Resistance L Cells (Cell Line) - drug effects L Cells (Cell Line) - metabolism Leucine - metabolism Mice Protein Biosynthesis RNA - biosynthesis Thymidine - metabolism Time Factors Tritium Uridine - metabolism |
| title | RESPONSE IN MACROMOLECULAR SYNTHESES OF MOUSE L CELLS TO BLEOMYCIN, WITH SPECIAL REFERENCE TO CELL-ANTIBIOTIC INTERACTION |
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