Development, validation, and utilization of a novel antibody specific to the type III chicken gonadotropin-releasing hormone receptor

Two gonadotropin-releasing hormone receptors (GnRH-Rs) have been characterized in chickens to date: cGnRH-R-I and cGnRH-R-III, with cGnRH-R-III being the predominant pituitary form. The purpose of the present study was to first validate a novel antibody for the specific detection of cGnRH-R-III and...

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Veröffentlicht in:	Domestic animal endocrinology 2011-02, Vol.40 (2), p.110-118
Hauptverfasser:	McFarlane, H.O., Joseph, N.T., Maddineni, S.R., Ramachandran, R., Bédécarrats, G.Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals anterior pituitary Antibodies - metabolism Avian Proteins - analysis Avian Proteins - metabolism biosynthesis Blotting, Western - methods cell lines Cercopithecus aethiops Chicken Chickens COS Cells detection Female female reproductive system gender differences GnRH gonadotropin-releasing hormone gonadotropin-releasing hormone III hens hormonal regulation hormone secretion immunocytochemistry Male male reproductive system Molecular Sequence Data Pituitary Pituitary Gland - metabolism pituitary gonadotropins polyclonal antibodies Receptor Receptors, LHRH - analysis Receptors, LHRH - metabolism Reproduction roosters Sequence Alignment temporal variation Western blotting
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