Primate RNA Tumor Virus-Like DNA Synthesized Endogenously by RNA-Dependent DNA Polymerase in Virus-Like Particles from Fresh Human Acute Leukemic Blood Cells
A particle of discrete biochemical composition was purified from fresh, unfrozen peripheral blood leukocytes of human patients with acute myeloblastic leukemia. This particle endogenously synthesized DNA by use of an RNA primer and template. About half of the DNA sequences synthesized in the presenc...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1973-11, Vol.70 (11), p.3219-3224 |
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| container_issue | 11 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 70 |
| creator | Gallo, R. C. Miller, N. R. Saxinger, W. C. Gillespie, D. |
| description | A particle of discrete biochemical composition was purified from fresh, unfrozen peripheral blood leukocytes of human patients with acute myeloblastic leukemia. This particle endogenously synthesized DNA by use of an RNA primer and template. About half of the DNA sequences synthesized in the presence of actinomycin D hybridized to RNA isolated from type-C sarcoma viruses of primates or mice; lower annealing values were obtained with RNA isolated from other sarcoma or leukemia viruses. The results confirm and extend previous results from molecular hybridization experiments related to the existence in human leukemia of components of RNA tumor viruses. |
| doi_str_mv | 10.1073/pnas.70.11.3219 |
| format | Article |
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C. ; Miller, N. R. ; Saxinger, W. C. ; Gillespie, D.</creator><creatorcontrib>Gallo, R. C. ; Miller, N. R. ; Saxinger, W. C. ; Gillespie, D.</creatorcontrib><description>A particle of discrete biochemical composition was purified from fresh, unfrozen peripheral blood leukocytes of human patients with acute myeloblastic leukemia. This particle endogenously synthesized DNA by use of an RNA primer and template. About half of the DNA sequences synthesized in the presence of actinomycin D hybridized to RNA isolated from type-C sarcoma viruses of primates or mice; lower annealing values were obtained with RNA isolated from other sarcoma or leukemia viruses. The results confirm and extend previous results from molecular hybridization experiments related to the existence in human leukemia of components of RNA tumor viruses.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.70.11.3219</identifier><identifier>PMID: 4131745</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Adult ; Avian Leukosis Virus - analysis ; Biological Sciences: Cell Biology ; DNA ; DNA, Viral - biosynthesis ; DNA, Viral - isolation & purification ; DNA, Viral - metabolism ; Electrophoresis, Polyacrylamide Gel ; Female ; Gammaretrovirus - analysis ; Gels ; Humans ; Leukemia ; Leukemia Virus, Feline - analysis ; Leukemia, Myeloid, Acute - microbiology ; Male ; Materials ; Middle Aged ; Murine leukemia virus ; Nucleic Acid Hybridization ; Oncogenic viruses ; Oncogenic Viruses - analysis ; Primates ; Rauscher Virus - analysis ; Retroviridae - analysis ; RNA ; RNA Viruses - isolation & purification ; RNA Viruses - metabolism ; RNA, Viral - isolation & purification ; RNA, Viral - metabolism ; RNA-Directed DNA Polymerase - metabolism ; Tritium ; Viruses</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1973-11, Vol.70 (11), p.3219-3224</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-7a669f557ae550d8cf06fb1b324beec0f6b06d1efce3b800b0ecbae91434c2953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/70/11.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/62956$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/62956$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4131745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallo, R. C.</creatorcontrib><creatorcontrib>Miller, N. R.</creatorcontrib><creatorcontrib>Saxinger, W. C.</creatorcontrib><creatorcontrib>Gillespie, D.</creatorcontrib><title>Primate RNA Tumor Virus-Like DNA Synthesized Endogenously by RNA-Dependent DNA Polymerase in Virus-Like Particles from Fresh Human Acute Leukemic Blood Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A particle of discrete biochemical composition was purified from fresh, unfrozen peripheral blood leukocytes of human patients with acute myeloblastic leukemia. This particle endogenously synthesized DNA by use of an RNA primer and template. About half of the DNA sequences synthesized in the presence of actinomycin D hybridized to RNA isolated from type-C sarcoma viruses of primates or mice; lower annealing values were obtained with RNA isolated from other sarcoma or leukemia viruses. The results confirm and extend previous results from molecular hybridization experiments related to the existence in human leukemia of components of RNA tumor viruses.</description><subject>Adult</subject><subject>Avian Leukosis Virus - analysis</subject><subject>Biological Sciences: Cell Biology</subject><subject>DNA</subject><subject>DNA, Viral - biosynthesis</subject><subject>DNA, Viral - isolation & purification</subject><subject>DNA, Viral - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Gammaretrovirus - analysis</subject><subject>Gels</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia Virus, Feline - analysis</subject><subject>Leukemia, Myeloid, Acute - microbiology</subject><subject>Male</subject><subject>Materials</subject><subject>Middle Aged</subject><subject>Murine leukemia virus</subject><subject>Nucleic Acid Hybridization</subject><subject>Oncogenic viruses</subject><subject>Oncogenic Viruses - analysis</subject><subject>Primates</subject><subject>Rauscher Virus - analysis</subject><subject>Retroviridae - analysis</subject><subject>RNA</subject><subject>RNA Viruses - isolation & purification</subject><subject>RNA Viruses - metabolism</subject><subject>RNA, Viral - isolation & purification</subject><subject>RNA, Viral - metabolism</subject><subject>RNA-Directed DNA Polymerase - metabolism</subject><subject>Tritium</subject><subject>Viruses</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EKtvCGQkJ5BOcsh0nzr8Dh2XbUqQVrKBwtRxn0nWb2IudVITvwnfFYZdVe-Fkjd_vjcfzCHnBYM4gT063Rvp5Hgo2T2JWPiIzBiWLMl7CYzIDiPOo4DF_So69vwGAMi3giBxxlrCcpzPye-10J3ukXz4t6NXQWUe_azf4aKVvkZ6Fy6-j6Tfo9S-s6bmp7TUaO_h2pNU4maIz3KKp0fR_6bVtxw6d9Ei1ud9qLV2vVYueNs529MKh39DLoZOGLtQQBljhcIudVvR9a21Nl9i2_hl50sjW4_P9eUK-XZxfLS-j1ecPH5eLVaR4xvool1lWNmmaS0xTqAvVQNZUrEpiXiEqaLIKspphozCpCoAKUFUSS8YTruIyTU7Iu13f7VB1WKvwGydbsZ1240ZhpRYPFaM34treCR7nMfDgf7P3O_tjQN-LTnsVfiANhmWJIg7ZQF4E8HQHKme9d9gc3mAgpkDFFKjIQ8HEFGhwvLo_2oHfJxj013t9Mv5THzR4-19ANEPb9vizD-TLHXnje-sOaBY2lCV_ANbnwEE</recordid><startdate>19731101</startdate><enddate>19731101</enddate><creator>Gallo, R. C.</creator><creator>Miller, N. R.</creator><creator>Saxinger, W. C.</creator><creator>Gillespie, D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19731101</creationdate><title>Primate RNA Tumor Virus-Like DNA Synthesized Endogenously by RNA-Dependent DNA Polymerase in Virus-Like Particles from Fresh Human Acute Leukemic Blood Cells</title><author>Gallo, R. C. ; Miller, N. R. ; Saxinger, W. C. ; Gillespie, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-7a669f557ae550d8cf06fb1b324beec0f6b06d1efce3b800b0ecbae91434c2953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Adult</topic><topic>Avian Leukosis Virus - analysis</topic><topic>Biological Sciences: Cell Biology</topic><topic>DNA</topic><topic>DNA, Viral - biosynthesis</topic><topic>DNA, Viral - isolation & purification</topic><topic>DNA, Viral - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Gammaretrovirus - analysis</topic><topic>Gels</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Leukemia Virus, Feline - analysis</topic><topic>Leukemia, Myeloid, Acute - microbiology</topic><topic>Male</topic><topic>Materials</topic><topic>Middle Aged</topic><topic>Murine leukemia virus</topic><topic>Nucleic Acid Hybridization</topic><topic>Oncogenic viruses</topic><topic>Oncogenic Viruses - analysis</topic><topic>Primates</topic><topic>Rauscher Virus - analysis</topic><topic>Retroviridae - analysis</topic><topic>RNA</topic><topic>RNA Viruses - isolation & purification</topic><topic>RNA Viruses - metabolism</topic><topic>RNA, Viral - isolation & purification</topic><topic>RNA, Viral - metabolism</topic><topic>RNA-Directed DNA Polymerase - metabolism</topic><topic>Tritium</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallo, R. C.</creatorcontrib><creatorcontrib>Miller, N. R.</creatorcontrib><creatorcontrib>Saxinger, W. C.</creatorcontrib><creatorcontrib>Gillespie, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallo, R. C.</au><au>Miller, N. R.</au><au>Saxinger, W. C.</au><au>Gillespie, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Primate RNA Tumor Virus-Like DNA Synthesized Endogenously by RNA-Dependent DNA Polymerase in Virus-Like Particles from Fresh Human Acute Leukemic Blood Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1973-11-01</date><risdate>1973</risdate><volume>70</volume><issue>11</issue><spage>3219</spage><epage>3224</epage><pages>3219-3224</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A particle of discrete biochemical composition was purified from fresh, unfrozen peripheral blood leukocytes of human patients with acute myeloblastic leukemia. This particle endogenously synthesized DNA by use of an RNA primer and template. About half of the DNA sequences synthesized in the presence of actinomycin D hybridized to RNA isolated from type-C sarcoma viruses of primates or mice; lower annealing values were obtained with RNA isolated from other sarcoma or leukemia viruses. The results confirm and extend previous results from molecular hybridization experiments related to the existence in human leukemia of components of RNA tumor viruses.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>4131745</pmid><doi>10.1073/pnas.70.11.3219</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1973-11, Vol.70 (11), p.3219-3224 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adult Avian Leukosis Virus - analysis Biological Sciences: Cell Biology DNA DNA, Viral - biosynthesis DNA, Viral - isolation & purification DNA, Viral - metabolism Electrophoresis, Polyacrylamide Gel Female Gammaretrovirus - analysis Gels Humans Leukemia Leukemia Virus, Feline - analysis Leukemia, Myeloid, Acute - microbiology Male Materials Middle Aged Murine leukemia virus Nucleic Acid Hybridization Oncogenic viruses Oncogenic Viruses - analysis Primates Rauscher Virus - analysis Retroviridae - analysis RNA RNA Viruses - isolation & purification RNA Viruses - metabolism RNA, Viral - isolation & purification RNA, Viral - metabolism RNA-Directed DNA Polymerase - metabolism Tritium Viruses |
| title | Primate RNA Tumor Virus-Like DNA Synthesized Endogenously by RNA-Dependent DNA Polymerase in Virus-Like Particles from Fresh Human Acute Leukemic Blood Cells |
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