Amplified Detection of DNA through an Autocatalytic and Catabolic DNAzyme-Mediated Process

Amplified Detection of DNA through an Autocatalytic and Catabolic DNAzyme-Mediated Process

Sense and sensibility: Amplified DNA detection is accomplished by the autocatalytic cleavage of a programmed nucleic acid structure based on the Mg2+‐dependent DNAzyme E6 (see picture). The analyte sequence is autocatalytically generated by the DNAzyme from a reporter nucleic acid, which is labeled...

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Veröffentlicht in:Angewandte Chemie International Edition 2011-01, Vol.50 (1), p.295-299
Hauptverfasser: Wang, Fuan, Elbaz, Johann, Teller, Carsten, Willner, Itamar
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
autocatalysis
Base Sequence
Biocatalysis
Biosensing Techniques - methods
Deoxyribonucleic acid
DNA
DNA - analysis
DNA - chemistry
DNA - metabolism
DNA, Catalytic - metabolism
DNAzymes
Fluorescence
Magnesium
Nucleic acids
sensors
Time Factors
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Beschreibung
Zusammenfassung:Sense and sensibility: Amplified DNA detection is accomplished by the autocatalytic cleavage of a programmed nucleic acid structure based on the Mg2+‐dependent DNAzyme E6 (see picture). The analyte sequence is autocatalytically generated by the DNAzyme from a reporter nucleic acid, which is labeled with a fluorophore and quencher at its two ends.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201005246