Rapid Online Nonenzymatic Protein Digestion Combining Microwave Heating Acid Hydrolysis and Electrochemical Oxidation

We report an online nonenzymatic method for site-specific digestion of proteins to yield peptides that are well suited for collision-induced dissociation tandem mass spectrometry. The method combines online microwave heating acid hydrolysis at aspartic acid and online electrochemical oxidation at tr...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-01, Vol.83 (1), p.359-367
Hauptverfasser:	Basile, Franco, Hauser, Nicolas
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Analytical chemistry Animals Biochemistry Cattle Chemistry Chromatography, Liquid Disulfides - chemistry Electrochemical methods Electrochemistry Escherichia coli - cytology Exact sciences and technology Hot Temperature Humans Hydrolysis Kinetics Mass spectrometry Microwaves Molecular Sequence Data Oxidation Oxidation-Reduction Peptide Fragments - chemistry Peptide Fragments - metabolism Peptides Proteins Proteins - chemistry Proteins - metabolism Spectrometric and optical methods Tandem Mass Spectrometry
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