Surface-Enhanced Raman Scattering Detection of DNA Derived from the West Nile Virus Genome Using Magnetic Capture of Raman-Active Gold Nanoparticles

A model paramagnetic nanoparticle (MNP) assay is demonstrated for surface-enhanced Raman scattering (SERS) detection of DNA oligonucleotides derived from the West Nile virus (WNV) genome. Detection is based on the capture of WNV target sequences by hybridization with complementary oligonucleotide pr...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-01, Vol.83 (1), p.254-260
Hauptverfasser:	Zhang, Hao, Harpster, Mark H, Park, Hee Joon, Johnson, Patrick A, Wilson, William C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Base Sequence Chemistry Deoxyribonucleic acid DNA DNA, Viral - analysis DNA, Viral - chemistry DNA, Viral - genetics Exact sciences and technology Genome, Viral - genetics Genomes Gold - chemistry Hybridization Lasers Light Limit of Detection Magnetics Metal Nanoparticles - chemistry Nanoparticles Nucleic Acid Hybridization Oligonucleotide Probes - chemistry Oligonucleotide Probes - genetics Photoelectron Spectroscopy Scattering, Radiation Spectrometric and optical methods Spectrum Analysis, Raman - methods Surface Properties Viruses West Nile virus - genetics
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