Inhibitory effect of Iboga-type indole alkaloids on capsaicin-induced contraction in isolated mouse rectum
Voacanga africana (Apocynaceae) is used as an anti-diarrheal medicine in West Africa. In the present study, we investigated the effect of an extract of V. africana and its constituents on smooth muscle contraction induced by capsaicin in mouse rectum, where transient receptor potential vanilloid typ...
Gespeichert in:
| Veröffentlicht in: | Journal of natural medicines 2011-01, Vol.65 (1), p.157-165 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 165 |
|---|---|
| container_issue | 1 |
| container_start_page | 157 |
| container_title | Journal of natural medicines |
| container_volume | 65 |
| creator | Lo, Mee Wah Matsumoto, Kenjiro Iwai, Masumi Tashima, Kimihito Kitajima, Mariko Horie, Syunji Takayama, Hiromitsu |
| description | Voacanga africana
(Apocynaceae) is used as an anti-diarrheal medicine in West Africa. In the present study, we investigated the effect of an extract of
V. africana
and its constituents on smooth muscle contraction induced by capsaicin in mouse rectum, where transient receptor potential vanilloid type 1 (TRPV1)-immunoreactive fibers are abundant. Methanol and alkaloid extracts of the root bark of
V. africana
were found to inhibit capsaicin-induced contraction in a dose-dependent manner (30–300 μg/ml). Major constituents isolated from the alkaloid extract were then studied for their effects on the capsaicin-induced contraction. The main active constituents were found to be Iboga-type alkaloids, including voacangine (
1
), 3-oxovoacangine (
2
), voacristine (
3
), and (7α)-voacangine hydroxyindolenine (
4
). The voacangine concentration dependently (3–100 μM) inhibited the capsaicin-induced contraction. The capsaicin-induced contraction was almost completely inhibited by the TRPV1 antagonist,
N
-(4-tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC). On the other hand, the Iboga-type alkaloids did not inhibit the contractions induced by 3 μM acetylcholine and 300 μM nicotine. These results suggest that Iboga-type alkaloids isolated from
V. africana
inhibit capsaicin-induced contraction in the mouse rectum, possibly via the inhibition of a TRPV1-mediated pathway. This inhibition may be involved in the anti-diarrheal effect of
V. africana
. |
| doi_str_mv | 10.1007/s11418-010-0478-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_821737024</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>821737024</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-104bf5b22826e16bdc6e612693e47e7863eb8fd6f64c386aac8309670ca820a73</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMotlZ_gBfJzVM0X03SoxQ_CgUveg7Z7GxN3d3UZPfQf2-k1aMwMEPed14mD0LXjN4xSvV9ZkwyQyijhEptiDpBU2YUI5QvxGmZhaRESCkm6CLnLaWSC8HO0YSzMhqlp2i76j9CFYaY9hiaBvyAY4NXVdw4Mux3gENfxxawaz9dG0Odceyxd7vsgg89KeroocY-9kNyfghFDaVybN1Q3rs4ZsCpxI7dJTprXJvh6thn6P3p8W35Qtavz6vlw5p4KeYDKadVzbzi3HAFTFW1V6AYVwsBUoM2SkBlmlo1SnphlHPeCLpQmnpnOHVazNDtIXeX4tcIebBdyB7a1vVQzrGGMy005bI42cHpU8w5QWN3KXQu7S2j9oewPRC2hbD9IWxV2bk5po9VB_Xfxi_SYuAHQy5Sv4Fkt3FMffnxP6nfDNGHTg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>821737024</pqid></control><display><type>article</type><title>Inhibitory effect of Iboga-type indole alkaloids on capsaicin-induced contraction in isolated mouse rectum</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Lo, Mee Wah ; Matsumoto, Kenjiro ; Iwai, Masumi ; Tashima, Kimihito ; Kitajima, Mariko ; Horie, Syunji ; Takayama, Hiromitsu</creator><creatorcontrib>Lo, Mee Wah ; Matsumoto, Kenjiro ; Iwai, Masumi ; Tashima, Kimihito ; Kitajima, Mariko ; Horie, Syunji ; Takayama, Hiromitsu</creatorcontrib><description>Voacanga africana
(Apocynaceae) is used as an anti-diarrheal medicine in West Africa. In the present study, we investigated the effect of an extract of
V. africana
and its constituents on smooth muscle contraction induced by capsaicin in mouse rectum, where transient receptor potential vanilloid type 1 (TRPV1)-immunoreactive fibers are abundant. Methanol and alkaloid extracts of the root bark of
V. africana
were found to inhibit capsaicin-induced contraction in a dose-dependent manner (30–300 μg/ml). Major constituents isolated from the alkaloid extract were then studied for their effects on the capsaicin-induced contraction. The main active constituents were found to be Iboga-type alkaloids, including voacangine (
1
), 3-oxovoacangine (
2
), voacristine (
3
), and (7α)-voacangine hydroxyindolenine (
4
). The voacangine concentration dependently (3–100 μM) inhibited the capsaicin-induced contraction. The capsaicin-induced contraction was almost completely inhibited by the TRPV1 antagonist,
N
-(4-tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC). On the other hand, the Iboga-type alkaloids did not inhibit the contractions induced by 3 μM acetylcholine and 300 μM nicotine. These results suggest that Iboga-type alkaloids isolated from
V. africana
inhibit capsaicin-induced contraction in the mouse rectum, possibly via the inhibition of a TRPV1-mediated pathway. This inhibition may be involved in the anti-diarrheal effect of
V. africana
.</description><identifier>ISSN: 1340-3443</identifier><identifier>EISSN: 1861-0293</identifier><identifier>DOI: 10.1007/s11418-010-0478-6</identifier><identifier>PMID: 21042867</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Animals ; Apocynaceae - chemistry ; Biomedical and Life Sciences ; Biomedicine ; Capsaicin - pharmacology ; Complementary & Alternative Medicine ; Immunohistochemistry ; In Vitro Techniques ; Indole Alkaloids - chemistry ; Indole Alkaloids - pharmacology ; Medicinal Chemistry ; Mice ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Original Paper ; Pharmacology/Toxicology ; Pharmacy ; Plant Sciences ; Rectum - drug effects ; TRPV Cation Channels - antagonists & inhibitors ; TRPV Cation Channels - metabolism</subject><ispartof>Journal of natural medicines, 2011-01, Vol.65 (1), p.157-165</ispartof><rights>The Japanese Society of Pharmacognosy and Springer 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-104bf5b22826e16bdc6e612693e47e7863eb8fd6f64c386aac8309670ca820a73</citedby><cites>FETCH-LOGICAL-c435t-104bf5b22826e16bdc6e612693e47e7863eb8fd6f64c386aac8309670ca820a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11418-010-0478-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11418-010-0478-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21042867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lo, Mee Wah</creatorcontrib><creatorcontrib>Matsumoto, Kenjiro</creatorcontrib><creatorcontrib>Iwai, Masumi</creatorcontrib><creatorcontrib>Tashima, Kimihito</creatorcontrib><creatorcontrib>Kitajima, Mariko</creatorcontrib><creatorcontrib>Horie, Syunji</creatorcontrib><creatorcontrib>Takayama, Hiromitsu</creatorcontrib><title>Inhibitory effect of Iboga-type indole alkaloids on capsaicin-induced contraction in isolated mouse rectum</title><title>Journal of natural medicines</title><addtitle>J Nat Med</addtitle><addtitle>J Nat Med</addtitle><description>Voacanga africana
(Apocynaceae) is used as an anti-diarrheal medicine in West Africa. In the present study, we investigated the effect of an extract of
V. africana
and its constituents on smooth muscle contraction induced by capsaicin in mouse rectum, where transient receptor potential vanilloid type 1 (TRPV1)-immunoreactive fibers are abundant. Methanol and alkaloid extracts of the root bark of
V. africana
were found to inhibit capsaicin-induced contraction in a dose-dependent manner (30–300 μg/ml). Major constituents isolated from the alkaloid extract were then studied for their effects on the capsaicin-induced contraction. The main active constituents were found to be Iboga-type alkaloids, including voacangine (
1
), 3-oxovoacangine (
2
), voacristine (
3
), and (7α)-voacangine hydroxyindolenine (
4
). The voacangine concentration dependently (3–100 μM) inhibited the capsaicin-induced contraction. The capsaicin-induced contraction was almost completely inhibited by the TRPV1 antagonist,
N
-(4-tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC). On the other hand, the Iboga-type alkaloids did not inhibit the contractions induced by 3 μM acetylcholine and 300 μM nicotine. These results suggest that Iboga-type alkaloids isolated from
V. africana
inhibit capsaicin-induced contraction in the mouse rectum, possibly via the inhibition of a TRPV1-mediated pathway. This inhibition may be involved in the anti-diarrheal effect of
V. africana
.</description><subject>Animals</subject><subject>Apocynaceae - chemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Capsaicin - pharmacology</subject><subject>Complementary & Alternative Medicine</subject><subject>Immunohistochemistry</subject><subject>In Vitro Techniques</subject><subject>Indole Alkaloids - chemistry</subject><subject>Indole Alkaloids - pharmacology</subject><subject>Medicinal Chemistry</subject><subject>Mice</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Original Paper</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Plant Sciences</subject><subject>Rectum - drug effects</subject><subject>TRPV Cation Channels - antagonists & inhibitors</subject><subject>TRPV Cation Channels - metabolism</subject><issn>1340-3443</issn><issn>1861-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlZ_gBfJzVM0X03SoxQ_CgUveg7Z7GxN3d3UZPfQf2-k1aMwMEPed14mD0LXjN4xSvV9ZkwyQyijhEptiDpBU2YUI5QvxGmZhaRESCkm6CLnLaWSC8HO0YSzMhqlp2i76j9CFYaY9hiaBvyAY4NXVdw4Mux3gENfxxawaz9dG0Odceyxd7vsgg89KeroocY-9kNyfghFDaVybN1Q3rs4ZsCpxI7dJTprXJvh6thn6P3p8W35Qtavz6vlw5p4KeYDKadVzbzi3HAFTFW1V6AYVwsBUoM2SkBlmlo1SnphlHPeCLpQmnpnOHVazNDtIXeX4tcIebBdyB7a1vVQzrGGMy005bI42cHpU8w5QWN3KXQu7S2j9oewPRC2hbD9IWxV2bk5po9VB_Xfxi_SYuAHQy5Sv4Fkt3FMffnxP6nfDNGHTg</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Lo, Mee Wah</creator><creator>Matsumoto, Kenjiro</creator><creator>Iwai, Masumi</creator><creator>Tashima, Kimihito</creator><creator>Kitajima, Mariko</creator><creator>Horie, Syunji</creator><creator>Takayama, Hiromitsu</creator><general>Springer Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110101</creationdate><title>Inhibitory effect of Iboga-type indole alkaloids on capsaicin-induced contraction in isolated mouse rectum</title><author>Lo, Mee Wah ; Matsumoto, Kenjiro ; Iwai, Masumi ; Tashima, Kimihito ; Kitajima, Mariko ; Horie, Syunji ; Takayama, Hiromitsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-104bf5b22826e16bdc6e612693e47e7863eb8fd6f64c386aac8309670ca820a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Apocynaceae - chemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Capsaicin - pharmacology</topic><topic>Complementary & Alternative Medicine</topic><topic>Immunohistochemistry</topic><topic>In Vitro Techniques</topic><topic>Indole Alkaloids - chemistry</topic><topic>Indole Alkaloids - pharmacology</topic><topic>Medicinal Chemistry</topic><topic>Mice</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Original Paper</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Plant Sciences</topic><topic>Rectum - drug effects</topic><topic>TRPV Cation Channels - antagonists & inhibitors</topic><topic>TRPV Cation Channels - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lo, Mee Wah</creatorcontrib><creatorcontrib>Matsumoto, Kenjiro</creatorcontrib><creatorcontrib>Iwai, Masumi</creatorcontrib><creatorcontrib>Tashima, Kimihito</creatorcontrib><creatorcontrib>Kitajima, Mariko</creatorcontrib><creatorcontrib>Horie, Syunji</creatorcontrib><creatorcontrib>Takayama, Hiromitsu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lo, Mee Wah</au><au>Matsumoto, Kenjiro</au><au>Iwai, Masumi</au><au>Tashima, Kimihito</au><au>Kitajima, Mariko</au><au>Horie, Syunji</au><au>Takayama, Hiromitsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of Iboga-type indole alkaloids on capsaicin-induced contraction in isolated mouse rectum</atitle><jtitle>Journal of natural medicines</jtitle><stitle>J Nat Med</stitle><addtitle>J Nat Med</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>65</volume><issue>1</issue><spage>157</spage><epage>165</epage><pages>157-165</pages><issn>1340-3443</issn><eissn>1861-0293</eissn><abstract>Voacanga africana
(Apocynaceae) is used as an anti-diarrheal medicine in West Africa. In the present study, we investigated the effect of an extract of
V. africana
and its constituents on smooth muscle contraction induced by capsaicin in mouse rectum, where transient receptor potential vanilloid type 1 (TRPV1)-immunoreactive fibers are abundant. Methanol and alkaloid extracts of the root bark of
V. africana
were found to inhibit capsaicin-induced contraction in a dose-dependent manner (30–300 μg/ml). Major constituents isolated from the alkaloid extract were then studied for their effects on the capsaicin-induced contraction. The main active constituents were found to be Iboga-type alkaloids, including voacangine (
1
), 3-oxovoacangine (
2
), voacristine (
3
), and (7α)-voacangine hydroxyindolenine (
4
). The voacangine concentration dependently (3–100 μM) inhibited the capsaicin-induced contraction. The capsaicin-induced contraction was almost completely inhibited by the TRPV1 antagonist,
N
-(4-tertiarybutylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC). On the other hand, the Iboga-type alkaloids did not inhibit the contractions induced by 3 μM acetylcholine and 300 μM nicotine. These results suggest that Iboga-type alkaloids isolated from
V. africana
inhibit capsaicin-induced contraction in the mouse rectum, possibly via the inhibition of a TRPV1-mediated pathway. This inhibition may be involved in the anti-diarrheal effect of
V. africana
.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>21042867</pmid><doi>10.1007/s11418-010-0478-6</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1340-3443 |
| ispartof | Journal of natural medicines, 2011-01, Vol.65 (1), p.157-165 |
| issn | 1340-3443 1861-0293 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_821737024 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Apocynaceae - chemistry Biomedical and Life Sciences Biomedicine Capsaicin - pharmacology Complementary & Alternative Medicine Immunohistochemistry In Vitro Techniques Indole Alkaloids - chemistry Indole Alkaloids - pharmacology Medicinal Chemistry Mice Muscle Contraction - drug effects Muscle, Smooth - drug effects Original Paper Pharmacology/Toxicology Pharmacy Plant Sciences Rectum - drug effects TRPV Cation Channels - antagonists & inhibitors TRPV Cation Channels - metabolism |
| title | Inhibitory effect of Iboga-type indole alkaloids on capsaicin-induced contraction in isolated mouse rectum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T06%3A02%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effect%20of%20Iboga-type%20indole%20alkaloids%20on%20capsaicin-induced%20contraction%20in%20isolated%20mouse%20rectum&rft.jtitle=Journal%20of%20natural%20medicines&rft.au=Lo,%20Mee%20Wah&rft.date=2011-01-01&rft.volume=65&rft.issue=1&rft.spage=157&rft.epage=165&rft.pages=157-165&rft.issn=1340-3443&rft.eissn=1861-0293&rft_id=info:doi/10.1007/s11418-010-0478-6&rft_dat=%3Cproquest_cross%3E821737024%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=821737024&rft_id=info:pmid/21042867&rfr_iscdi=true |