An activated medium with high durability and low nonspecific adsorption: Application to protein A chromatography

Activated media allow the user to easily synthesize a variety of affinity media. We have developed a novel activated medium based on porous silica modified with phosphorylcholine (PC) and N-hydroxysuccinimide (NHS) groups for the purpose of high-throughput purification and reducing nonspecific prote...

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Veröffentlicht in:	Analytical biochemistry 2011-02, Vol.409 (1), p.123-129
Hauptverfasser:	Maeno, Katsuyuki, Hirayama, Aya, Sakuma, Kenichi, Miyazawa, Kazuyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Affinity Chromatography, Affinity - methods High throughput Nonspecific Phosphorylcholine Phosphorylcholine - chemistry Protein A Protein Binding Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Silicon Dioxide - chemistry Staphylococcal Protein A - chemistry Staphylococcal Protein A - genetics Staphylococcal Protein A - metabolism Succinimides - chemistry
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