Carbodiimide EDC Induces Cross-Links That Stabilize RNase A C-Dimer against Dissociation: EDC Adducts Can Affect Protein Net Charge, Conformation, and Activity

RNase A self-associates under certain conditions to form a series of domain-swapped oligomers. These oligomers show high catalytic activity against double-stranded RNA and striking antitumor actions that are lacking in the monomer. However, the dissociation of these metastable oligomers limits their...

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Veröffentlicht in:	Bioconjugate chemistry 2009-08, Vol.20 (8), p.1459-1473
Hauptverfasser:	López-Alonso, Jorge P, Diez-García, Fernando, Font, Josep, Ribó, Marc, Vilanova, Maria, Scholtz, J. Martin, González, Carlos, Vottariello, Francesca, Gotte, Giovanni, Libonati, Massimo, Laurents, Douglas V
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Carbodiimides - chemistry Chemical bonds Chromatography Cross-Linking Reagents - chemistry Dimerization Mass spectrometry Models, Molecular Molecular Structure Proteins Ribonuclease, Pancreatic - chemistry Ribonuclease, Pancreatic - metabolism Ribonucleic acid RNA Stereoisomerism
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