Ligand-mediated osmium binding: Its application in coating biological specimens for scanning electron microscopy
The osmium-bridging properties of thiocarbohydrazide have been used to bind additional osmium to a variety of soft tissue surfaces before critical-point drying. The procedure gives satisfactory protection to specimens under a scanning electron beam at accelerating voltages between 5 and 25 kV and pr...
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| Veröffentlicht in: | Journal of ultrastructure research 1973-11, Vol.45 (3), p.254-258 |
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| container_end_page | 258 |
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| container_issue | 3 |
| container_start_page | 254 |
| container_title | Journal of ultrastructure research |
| container_volume | 45 |
| creator | Kelley, Robert O. Dekker, Ronald A.F. Bluemink, John G. |
| description | The osmium-bridging properties of thiocarbohydrazide have been used to bind additional osmium to a variety of soft tissue surfaces before critical-point drying. The procedure gives satisfactory protection to specimens under a scanning electron beam at accelerating voltages between 5 and 25 kV and provides the microscopist with a simple, efficient, and economical alternative to evaporative coating with other heavy metals prior to analysis in the scanning electron microscope. |
| doi_str_mv | 10.1016/S0022-5320(73)80051-6 |
| format | Article |
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| ispartof | Journal of ultrastructure research, 1973-11, Vol.45 (3), p.254-258 |
| issn | 0022-5320 1878-2345 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Evaluation Studies as Topic Histological Techniques - standards Hydrazines Ligands Methods Mice Microscopy, Electron, Scanning Osmium Sulfides Xenopus |
| title | Ligand-mediated osmium binding: Its application in coating biological specimens for scanning electron microscopy |
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