Some homogeneity studies on commercial preparations of allegedly crystalline phosphomannose isomerase

Commercial preparations of what is labeled as “Crystalline Phosphomannose Isomerase” were subjected to chromatographic, electrophoretic and ultracentrifugal analysis to determine the degree of their homogeneity. The results suggest that these preparations do not contain more than 5 to 10% of their t...

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Veröffentlicht in:	Biochemical and biophysical research communications 1973-12, Vol.55 (3), p.636-641
Hauptverfasser:	Feramisco, J.R., Tilley, B.E., Conn, W.R., Gracy, R.W., Noltmann, E.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrate Epimerases - analysis Carbohydrate Epimerases - isolation & purification Chromatography, DEAE-Cellulose Crystallization Electrophoresis, Polyacrylamide Gel Evaluation Studies as Topic Hexosephosphates Mannose Methods Saccharomyces cerevisiae - enzymology Spectrophotometry, Ultraviolet Ultracentrifugation
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