Sensitive determination of a pharmaceutical compound and its metabolites in human plasma by ultra-high performance liquid chromatography–tandem mass spectrometry with on-line solid-phase extraction

This paper describes the determination of a drug candidate and two metabolites in human plasma by column-switching LC–MS/MS after protein precipitation. Starting from a standard method with a quantitation limit of 0.5ng/mL, a highly sensitive assay was developed, employing UHPLC separation and detec...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 2011-03, Vol.54 (4), p.742-749
Hauptverfasser:	Heinig, K., Wirz, T., Bucheli, F., Monin, V., Gloge, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Calibration Chromatography, High Pressure Liquid - methods drugs Fundamental and applied biological sciences. Psychology General pharmacology High-Throughput Screening Assays Human plasma Humans LC–MS/MS Limit of Detection liquid chromatography Medical sciences metabolites Microchemistry - methods Microdosing Molecular Structure On-line SPE Pharmaceutical Preparations - administration & dosage Pharmaceutical Preparations - blood Pharmaceutical Preparations - chemistry Pharmacokinetics Pharmacology. Drug treatments Reproducibility of Results solid phase extraction Solid Phase Microextraction spectrometers Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Technology, Pharmaceutical UHPLC
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!