Determination of urinary amino acids by gas chromatography

We have used gas chromatography to measure amino acids in hydrolysates of urine. Pigments were removed by adsorption on activated charcoal-resin which has been treated with salicylic acid. Nor-leucine was added as an internal standard. The amino acids were converted to their n-propyl- N-acetyl ester...

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Veröffentlicht in:	Clinica chimica acta 1973-09, Vol.48 (1), p.65-75
Hauptverfasser:	McGregor, Robert F., Brittin, Geoffrey M., Sharon, Mary S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - urine Aminopeptidases Charcoal Chemical Phenomena Chemistry, Physical Chromatography, Gas Esters - urine Humans Hydrolysis Ion Exchange Methionine - urine Salicylates Silicon Dioxide Talc
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creator	McGregor, Robert F. Brittin, Geoffrey M. Sharon, Mary S.
description	We have used gas chromatography to measure amino acids in hydrolysates of urine. Pigments were removed by adsorption on activated charcoal-resin which has been treated with salicylic acid. Nor-leucine was added as an internal standard. The amino acids were converted to their n-propyl- N-acetyl esters, which were separated by gas chromatography and quantitated by a flame ionization detector and an electronic integrator. Although overnight hydrolysis of urine and esterification and acetylation of the amino acids were time-consuming, these operations were well suited to batch processing, so that a single technologist could routinely analyze 10 urine samples per day with a single gas Chromatograph. The method quantitated 16 amino acids in a single Chromatographie run. The precision of analysis of specific amino acids was excellent (coefficient of variation
doi_str_mv	10.1016/0009-8981(73)90218-0
format	Article
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Pigments were removed by adsorption on activated charcoal-resin which has been treated with salicylic acid. Nor-leucine was added as an internal standard. The amino acids were converted to their n-propyl- N-acetyl esters, which were separated by gas chromatography and quantitated by a flame ionization detector and an electronic integrator. Although overnight hydrolysis of urine and esterification and acetylation of the amino acids were time-consuming, these operations were well suited to batch processing, so that a single technologist could routinely analyze 10 urine samples per day with a single gas Chromatograph. The method quantitated 16 amino acids in a single Chromatographie run. The precision of analysis of specific amino acids was excellent (coefficient of variation <5%). Recoveries were variable for glycine, but were excellent for other amino acids.</description><subject>Amino Acids - urine</subject><subject>Aminopeptidases</subject><subject>Charcoal</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Chromatography, Gas</subject><subject>Esters - urine</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Ion Exchange</subject><subject>Methionine - urine</subject><subject>Salicylates</subject><subject>Silicon Dioxide</subject><subject>Talc</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9PwzAMxSMEGmPwDUDqCcGh4DRtk3JAQuOvNIkLnKMkdbegdRlJi7RvT8qmHTlZz-_Zln-EnFO4oUDLWwCoUlEJesXZdQUZFSkckDEVnKUsr7JDMt5HjslJCF9R5lDSERnlrCh4Rsfk7hE79K1dqc66VeKapPdR-E2iYtMlytg6JHqTzFVIzMK7VnVu7tV6sTklR41aBjzb1Qn5fH76mL6ms_eXt-nDLDWs4F2KRYOi1oIjw0KJGihlyKMqwRgoUWvDhRClECAgMxk1VaV1RotGq5JjxSbkcrt37d13j6GTrQ0Gl0u1QtcHKTLgRV4MwXwbNN6F4LGRa2_b-IukIAdkcuAhBx6SM_mHTEIcu9jt73WL9X5oxyj691sf45M_Fr0MxuLKYG09mk7Wzv5_4BfcKnpv</recordid><startdate>19730928</startdate><enddate>19730928</enddate><creator>McGregor, Robert F.</creator><creator>Brittin, Geoffrey M.</creator><creator>Sharon, Mary S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19730928</creationdate><title>Determination of urinary amino acids by gas chromatography</title><author>McGregor, Robert F. ; Brittin, Geoffrey M. ; Sharon, Mary S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-e5fe8db87e3e5a8d0113e77e360cc06ebbc78886880802c21c99bb215fba67e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Amino Acids - urine</topic><topic>Aminopeptidases</topic><topic>Charcoal</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Chromatography, Gas</topic><topic>Esters - urine</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Ion Exchange</topic><topic>Methionine - urine</topic><topic>Salicylates</topic><topic>Silicon Dioxide</topic><topic>Talc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McGregor, Robert F.</creatorcontrib><creatorcontrib>Brittin, Geoffrey M.</creatorcontrib><creatorcontrib>Sharon, Mary S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McGregor, Robert F.</au><au>Brittin, Geoffrey M.</au><au>Sharon, Mary S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of urinary amino acids by gas chromatography</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1973-09-28</date><risdate>1973</risdate><volume>48</volume><issue>1</issue><spage>65</spage><epage>75</epage><pages>65-75</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>We have used gas chromatography to measure amino acids in hydrolysates of urine. Pigments were removed by adsorption on activated charcoal-resin which has been treated with salicylic acid. Nor-leucine was added as an internal standard. The amino acids were converted to their n-propyl- N-acetyl esters, which were separated by gas chromatography and quantitated by a flame ionization detector and an electronic integrator. Although overnight hydrolysis of urine and esterification and acetylation of the amino acids were time-consuming, these operations were well suited to batch processing, so that a single technologist could routinely analyze 10 urine samples per day with a single gas Chromatograph. The method quantitated 16 amino acids in a single Chromatographie run. The precision of analysis of specific amino acids was excellent (coefficient of variation <5%). 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subjects	Amino Acids - urine Aminopeptidases Charcoal Chemical Phenomena Chemistry, Physical Chromatography, Gas Esters - urine Humans Hydrolysis Ion Exchange Methionine - urine Salicylates Silicon Dioxide Talc
title	Determination of urinary amino acids by gas chromatography
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