Simple method for differential staining of paraffin embedded plant material using toluidine blue O
Differential staining of paraffin embedded plant material is accomplished without removing the paraffin. Material fixed in 3% glutaraldehyde in 0.02 M phosphate buffer is dehydrated, embedded, sectioned, and mounted on glass slides using conventional methods. The slides are placed in 0.05% toluidine...
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| Veröffentlicht in: | Biotechnic & histochemistry 1973-01, Vol.48 (5), p.247-249 |
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| container_issue | 5 |
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| container_title | Biotechnic & histochemistry |
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| creator | Sakai, W.S |
| description | Differential staining of paraffin embedded plant material is accomplished without removing the paraffin. Material fixed in 3% glutaraldehyde in 0.02 M phosphate buffer is dehydrated, embedded, sectioned, and mounted on glass slides using conventional methods. The slides are placed in 0.05% toluidine blue O in distilled water for 2-30 min, rinsed in water for 1 min, and allowed to air dry. Paraffin is removed with 2 changes of xylene and the cover slip mounted with resin. Lignified tissue, suberized tissue, and some tannins are stained blue-green, while nonlignified walls are stained red-purple. An advantage of the method is the short time required for staining and mounting. Also, critical counterstaining is not required. |
| doi_str_mv | 10.3109/10520297309116632 |
| format | Article |
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Material fixed in 3% glutaraldehyde in 0.02 M phosphate buffer is dehydrated, embedded, sectioned, and mounted on glass slides using conventional methods. The slides are placed in 0.05% toluidine blue O in distilled water for 2-30 min, rinsed in water for 1 min, and allowed to air dry. Paraffin is removed with 2 changes of xylene and the cover slip mounted with resin. Lignified tissue, suberized tissue, and some tannins are stained blue-green, while nonlignified walls are stained red-purple. An advantage of the method is the short time required for staining and mounting. 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Also, critical counterstaining is not required.</description><subject>botany</subject><subject>Glutaral</subject><subject>Methods</subject><subject>Paraffin</subject><subject>Plant Cells</subject><subject>Plant Science and Plant Products</subject><subject>Staining and Labeling</subject><subject>Tolonium Chloride</subject><issn>0038-9153</issn><issn>1052-0295</issn><issn>1473-7760</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9v1DAQxS0EKkvhA3BA-MQtMLbzz4ILqqAgVeqh9BxN7HHXlRMH21HVb09Wu0JCCE5zmPfevPkx9lrAeyVAfxDQSJC6U6CFaFsln7CdqDtVdV0LT9kOQPWVFo16zl7kfA8AfQ_yjJ3VQratFjs23vhpCcQnKvtouYuJW-8cJZqLx8BzQT_7-Y5HxxdM6JyfOU0jWUuWLwHnwicslA7iNR-UJYbVWz8TH8NK_Pole-YwZHp1mufs9uuXHxffqqvry-8Xn68q08imVLUBpE6NRmpDbW1s53pllJWjrLfWNY6dNr0FgaQbK5TqARVqDXZEI6hR5-zdMXdJ8edKuQyTz4bC1pHimodeQtO2UmxCcRSaFHNO5IYl-QnT4yBgOHAd_uK6ed6cwtdxIvvbcQK57T8d937eGE74EFOwQ8HHEJNLOBufD9H_jv_4h31PGMreYKLhPq5p3rj9t9zbo9thHPAubbdubyQIBXJ7V_ZK_QI6cqJA</recordid><startdate>19730101</startdate><enddate>19730101</enddate><creator>Sakai, W.S</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19730101</creationdate><title>Simple method for differential staining of paraffin embedded plant material using toluidine blue O</title><author>Sakai, W.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-4c0ae73bc29ce64cd7f83c3d2b248804ab79c8d01ae95d13380a3a990dbac1e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>botany</topic><topic>Glutaral</topic><topic>Methods</topic><topic>Paraffin</topic><topic>Plant Cells</topic><topic>Plant Science and Plant Products</topic><topic>Staining and Labeling</topic><topic>Tolonium Chloride</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakai, W.S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnic & histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakai, W.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple method for differential staining of paraffin embedded plant material using toluidine blue O</atitle><jtitle>Biotechnic & histochemistry</jtitle><addtitle>Stain Technol</addtitle><date>1973-01-01</date><risdate>1973</risdate><volume>48</volume><issue>5</issue><spage>247</spage><epage>249</epage><pages>247-249</pages><issn>0038-9153</issn><issn>1052-0295</issn><eissn>1473-7760</eissn><abstract>Differential staining of paraffin embedded plant material is accomplished without removing the paraffin. Material fixed in 3% glutaraldehyde in 0.02 M phosphate buffer is dehydrated, embedded, sectioned, and mounted on glass slides using conventional methods. The slides are placed in 0.05% toluidine blue O in distilled water for 2-30 min, rinsed in water for 1 min, and allowed to air dry. Paraffin is removed with 2 changes of xylene and the cover slip mounted with resin. Lignified tissue, suberized tissue, and some tannins are stained blue-green, while nonlignified walls are stained red-purple. An advantage of the method is the short time required for staining and mounting. Also, critical counterstaining is not required.</abstract><cop>United States</cop><pub>Informa UK Ltd</pub><pmid>4126691</pmid><doi>10.3109/10520297309116632</doi><tpages>3</tpages></addata></record> |
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| ispartof | Biotechnic & histochemistry, 1973-01, Vol.48 (5), p.247-249 |
| issn | 0038-9153 1052-0295 1473-7760 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_82056621 |
| source | Taylor & Francis:Master (3349 titles); MEDLINE |
| subjects | botany Glutaral Methods Paraffin Plant Cells Plant Science and Plant Products Staining and Labeling Tolonium Chloride |
| title | Simple method for differential staining of paraffin embedded plant material using toluidine blue O |
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