Tissue Plate: A New Technique for Long-Term Organotypic Culture
A technique is described in which kidneys of 14- and 18-day-old fetal chicks, or hatched chicks, are dissociated either partially or totally into individual cells. In either case the cells are sedimented and subsequently cultured on a three-dimensional substrate. This approach leads to formation of...
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| Veröffentlicht in: | In vitro 1973-05, Vol.8 (6), p.483-488 |
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| container_title | In vitro |
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| creator | Ansevin, Krystyna D. Lipps, Binie V. |
| description | A technique is described in which kidneys of 14- and 18-day-old fetal chicks, or hatched chicks, are dissociated either partially or totally into individual cells. In either case the cells are sedimented and subsequently cultured on a three-dimensional substrate. This approach leads to formation of gigantic aggregates, "tissue plates," which undergo excellent histogenesis and remain differentiated for several weeks. Eventually an increase in connective tissue occurs; however, organ specific structures persist in the tissue plates even after 6 weeks of cultivation. |
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In either case the cells are sedimented and subsequently cultured on a three-dimensional substrate. This approach leads to formation of gigantic aggregates, "tissue plates," which undergo excellent histogenesis and remain differentiated for several weeks. Eventually an increase in connective tissue occurs; however, organ specific structures persist in the tissue plates even after 6 weeks of cultivation.</description><identifier>ISSN: 0073-5655</identifier><identifier>PMID: 4725695</identifier><language>eng</language><publisher>United States: Tissue Culture Association, Inc</publisher><subject>Animals ; Cell aggregates ; Cell Differentiation ; Chick Embryo ; Chickens ; Chicks ; Connective tissues ; Cultured cells ; Female ; Histocytochemistry ; Kidney - cytology ; Kidney cells ; Kidneys ; Liver cells ; Morphogenesis ; Organ Culture Techniques ; Organ Specificity ; Pregnancy ; Time Factors ; Tissue culture techniques</subject><ispartof>In vitro, 1973-05, Vol.8 (6), p.483-488</ispartof><rights>Copyright 1973 Tissue Culture Association</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4291700$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4291700$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4725695$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ansevin, Krystyna D.</creatorcontrib><creatorcontrib>Lipps, Binie V.</creatorcontrib><title>Tissue Plate: A New Technique for Long-Term Organotypic Culture</title><title>In vitro</title><addtitle>In Vitro</addtitle><description>A technique is described in which kidneys of 14- and 18-day-old fetal chicks, or hatched chicks, are dissociated either partially or totally into individual cells. 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Eventually an increase in connective tissue occurs; however, organ specific structures persist in the tissue plates even after 6 weeks of cultivation.</description><subject>Animals</subject><subject>Cell aggregates</subject><subject>Cell Differentiation</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Chicks</subject><subject>Connective tissues</subject><subject>Cultured cells</subject><subject>Female</subject><subject>Histocytochemistry</subject><subject>Kidney - cytology</subject><subject>Kidney cells</subject><subject>Kidneys</subject><subject>Liver cells</subject><subject>Morphogenesis</subject><subject>Organ Culture Techniques</subject><subject>Organ Specificity</subject><subject>Pregnancy</subject><subject>Time Factors</subject><subject>Tissue culture techniques</subject><issn>0073-5655</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtqwzAQRbVoSdO0f9CCVt0ZJFsjS92UEPqC0HThro2kjFIbvyrZlPx9DQl0NTDncJm5F2TJWJ4lIAGuyHWMNWMZkylfkIXIU5AaluSpqGKckH42ZsRHuqYf-EsLdN9d9TOvfR_otu8OSYGhpbtwMF0_HofK0c3UjFPAG3LpTRPx9jxX5Ovludi8Jdvd6_tmvU1qDnpMABC0YM5zL_fCepUJtgcjjPTKgpNCWGBWeMiszplTAgTXKboUtXLMptmKPJxyh9DPh8WxbKvosGlMh_0US8W1yjWXs3h_Fifb4r4cQtWacCzPH8_87sTrOPbhH6ea53M_fwyyWRM</recordid><startdate>197305</startdate><enddate>197305</enddate><creator>Ansevin, Krystyna D.</creator><creator>Lipps, Binie V.</creator><general>Tissue Culture Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>197305</creationdate><title>Tissue Plate: A New Technique for Long-Term Organotypic Culture</title><author>Ansevin, Krystyna D. ; Lipps, Binie V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j159t-55e5940cf1f6d4bf8340d5a4a6f8b5c644b50b4f53b970c8454192ec2e98c0b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Cell aggregates</topic><topic>Cell Differentiation</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Chicks</topic><topic>Connective tissues</topic><topic>Cultured cells</topic><topic>Female</topic><topic>Histocytochemistry</topic><topic>Kidney - cytology</topic><topic>Kidney cells</topic><topic>Kidneys</topic><topic>Liver cells</topic><topic>Morphogenesis</topic><topic>Organ Culture Techniques</topic><topic>Organ Specificity</topic><topic>Pregnancy</topic><topic>Time Factors</topic><topic>Tissue culture techniques</topic><toplevel>online_resources</toplevel><creatorcontrib>Ansevin, Krystyna D.</creatorcontrib><creatorcontrib>Lipps, Binie V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>In vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ansevin, Krystyna D.</au><au>Lipps, Binie V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue Plate: A New Technique for Long-Term Organotypic Culture</atitle><jtitle>In vitro</jtitle><addtitle>In Vitro</addtitle><date>1973-05</date><risdate>1973</risdate><volume>8</volume><issue>6</issue><spage>483</spage><epage>488</epage><pages>483-488</pages><issn>0073-5655</issn><abstract>A technique is described in which kidneys of 14- and 18-day-old fetal chicks, or hatched chicks, are dissociated either partially or totally into individual cells. In either case the cells are sedimented and subsequently cultured on a three-dimensional substrate. This approach leads to formation of gigantic aggregates, "tissue plates," which undergo excellent histogenesis and remain differentiated for several weeks. Eventually an increase in connective tissue occurs; however, organ specific structures persist in the tissue plates even after 6 weeks of cultivation.</abstract><cop>United States</cop><pub>Tissue Culture Association, Inc</pub><pmid>4725695</pmid><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0073-5655 |
| ispartof | In vitro, 1973-05, Vol.8 (6), p.483-488 |
| issn | 0073-5655 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81987916 |
| source | JSTOR Archive Collection A-Z Listing; MEDLINE |
| subjects | Animals Cell aggregates Cell Differentiation Chick Embryo Chickens Chicks Connective tissues Cultured cells Female Histocytochemistry Kidney - cytology Kidney cells Kidneys Liver cells Morphogenesis Organ Culture Techniques Organ Specificity Pregnancy Time Factors Tissue culture techniques |
| title | Tissue Plate: A New Technique for Long-Term Organotypic Culture |
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