Effects of preparation method on reversible conformational changes induced by neutral salts in spin-labeled erythrocyte membranes
Addition of “chaotropic” neutral salts to spin-labeled erythrocyte membranes produced changes in membrane conformation proportional to the known activities of the salts: KSCN > CaCl 2 ≥ KNO 3 ⪢ NaCl. The effects had a threshold of about 0.10 m and increased through 0.60 m for all salts. Above 0.6...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1973-06, Vol.156 (2), p.653-657 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Kirkpatrick, Francis H. Sandberg, Howard E. |
| description | Addition of “chaotropic” neutral salts to spin-labeled erythrocyte membranes produced changes in membrane conformation proportional to the known activities of the salts: KSCN > CaCl
2 ≥ KNO
3 ⪢ NaCl. The effects had a threshold of about 0.10
m and increased through 0.60
m for all salts. Above 0.6
m other changes due to dielectric effects and/or protein loss began.
The chaotropic effects were reversible on removal of the chaotropic ion, for human (or bovine) membranes prepared with a buffer ionic strength of 0.02. For membranes prepared with a buffer ionic strength of 0.005 the changes were irreversible. Exposure to salt concentration greater than 1
m also produced irreversible changes, probably due to solubilization. The results appear to show that the water-soluble membrane proteins are essential to maintain the “native” membrane conformation. |
| doi_str_mv | 10.1016/0003-9861(73)90317-2 |
| format | Article |
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2 ≥ KNO
3 ⪢ NaCl. The effects had a threshold of about 0.10
m and increased through 0.60
m for all salts. Above 0.6
m other changes due to dielectric effects and/or protein loss began.
The chaotropic effects were reversible on removal of the chaotropic ion, for human (or bovine) membranes prepared with a buffer ionic strength of 0.02. For membranes prepared with a buffer ionic strength of 0.005 the changes were irreversible. Exposure to salt concentration greater than 1
m also produced irreversible changes, probably due to solubilization. The results appear to show that the water-soluble membrane proteins are essential to maintain the “native” membrane conformation.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(73)90317-2</identifier><identifier>PMID: 4352421</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blood Proteins - physiology ; Calcium Chloride - pharmacology ; Cattle ; Cell Membrane - drug effects ; Electron Spin Resonance Spectroscopy ; Erythrocytes - cytology ; Erythrocytes - drug effects ; Erythrocytes - physiology ; Humans ; Maleimides ; Nitrates - pharmacology ; Osmolar Concentration ; Potassium - pharmacology ; Sodium Chloride - pharmacology ; Thiocyanates - pharmacology</subject><ispartof>Archives of biochemistry and biophysics, 1973-06, Vol.156 (2), p.653-657</ispartof><rights>1973</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-6a69181cb3429fec58951a2576116b4ff6c1c821af687af818ab97bbf0c0ebf23</citedby><cites>FETCH-LOGICAL-c357t-6a69181cb3429fec58951a2576116b4ff6c1c821af687af818ab97bbf0c0ebf23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(73)90317-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4352421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kirkpatrick, Francis H.</creatorcontrib><creatorcontrib>Sandberg, Howard E.</creatorcontrib><title>Effects of preparation method on reversible conformational changes induced by neutral salts in spin-labeled erythrocyte membranes</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>Addition of “chaotropic” neutral salts to spin-labeled erythrocyte membranes produced changes in membrane conformation proportional to the known activities of the salts: KSCN > CaCl
2 ≥ KNO
3 ⪢ NaCl. The effects had a threshold of about 0.10
m and increased through 0.60
m for all salts. Above 0.6
m other changes due to dielectric effects and/or protein loss began.
The chaotropic effects were reversible on removal of the chaotropic ion, for human (or bovine) membranes prepared with a buffer ionic strength of 0.02. For membranes prepared with a buffer ionic strength of 0.005 the changes were irreversible. Exposure to salt concentration greater than 1
m also produced irreversible changes, probably due to solubilization. The results appear to show that the water-soluble membrane proteins are essential to maintain the “native” membrane conformation.</description><subject>Animals</subject><subject>Blood Proteins - physiology</subject><subject>Calcium Chloride - pharmacology</subject><subject>Cattle</subject><subject>Cell Membrane - drug effects</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Erythrocytes - cytology</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - physiology</subject><subject>Humans</subject><subject>Maleimides</subject><subject>Nitrates - pharmacology</subject><subject>Osmolar Concentration</subject><subject>Potassium - pharmacology</subject><subject>Sodium Chloride - pharmacology</subject><subject>Thiocyanates - pharmacology</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-LFDEQxYMo67j6DRRyEj20ptLd6eQiyLL-gQUveg5JuuJEujttkl6Yo9_ctDPs0VMVvFevKr8Q8hLYO2Ag3jPG2kZJAW-G9q1iLQwNf0QOwJRoWCu7x-TwYHlKnuX8izGATvArctW1Pe84HMifW-_RlUyjp2vC1SRTQlzojOUYR1q7hPeYcrATUhcXH9P8z2Em6o5m-YmZhmXcHI7UnuiCW0lVymYqu0DzGpZmMhanasB0KscU3algXTDbZBbMz8kTb6aMLy71mvz4dPv95ktz9-3z15uPd41r-6E0wggFEpxtO67qyb1UPRjeDwJA2M574cBJDsYLORgvQRqrBms9cwyt5-01eX3OXVP8vWEueg7Z4TTVI-KWtQQ1MOCqGruz0aWYc0Kv1xRmk04amN7J6x2r3rHqodadvN7zX13yNzvj-DB0QV31D2cd6yPvAyadXcClggup_oAeY_j_gr8VUJWQ</recordid><startdate>197306</startdate><enddate>197306</enddate><creator>Kirkpatrick, Francis H.</creator><creator>Sandberg, Howard E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197306</creationdate><title>Effects of preparation method on reversible conformational changes induced by neutral salts in spin-labeled erythrocyte membranes</title><author>Kirkpatrick, Francis H. ; Sandberg, Howard E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-6a69181cb3429fec58951a2576116b4ff6c1c821af687af818ab97bbf0c0ebf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Blood Proteins - physiology</topic><topic>Calcium Chloride - pharmacology</topic><topic>Cattle</topic><topic>Cell Membrane - drug effects</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Erythrocytes - cytology</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - physiology</topic><topic>Humans</topic><topic>Maleimides</topic><topic>Nitrates - pharmacology</topic><topic>Osmolar Concentration</topic><topic>Potassium - pharmacology</topic><topic>Sodium Chloride - pharmacology</topic><topic>Thiocyanates - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kirkpatrick, Francis H.</creatorcontrib><creatorcontrib>Sandberg, Howard E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kirkpatrick, Francis H.</au><au>Sandberg, Howard E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of preparation method on reversible conformational changes induced by neutral salts in spin-labeled erythrocyte membranes</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1973-06</date><risdate>1973</risdate><volume>156</volume><issue>2</issue><spage>653</spage><epage>657</epage><pages>653-657</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>Addition of “chaotropic” neutral salts to spin-labeled erythrocyte membranes produced changes in membrane conformation proportional to the known activities of the salts: KSCN > CaCl
2 ≥ KNO
3 ⪢ NaCl. The effects had a threshold of about 0.10
m and increased through 0.60
m for all salts. Above 0.6
m other changes due to dielectric effects and/or protein loss began.
The chaotropic effects were reversible on removal of the chaotropic ion, for human (or bovine) membranes prepared with a buffer ionic strength of 0.02. For membranes prepared with a buffer ionic strength of 0.005 the changes were irreversible. Exposure to salt concentration greater than 1
m also produced irreversible changes, probably due to solubilization. The results appear to show that the water-soluble membrane proteins are essential to maintain the “native” membrane conformation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4352421</pmid><doi>10.1016/0003-9861(73)90317-2</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1973-06, Vol.156 (2), p.653-657 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81970129 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Blood Proteins - physiology Calcium Chloride - pharmacology Cattle Cell Membrane - drug effects Electron Spin Resonance Spectroscopy Erythrocytes - cytology Erythrocytes - drug effects Erythrocytes - physiology Humans Maleimides Nitrates - pharmacology Osmolar Concentration Potassium - pharmacology Sodium Chloride - pharmacology Thiocyanates - pharmacology |
| title | Effects of preparation method on reversible conformational changes induced by neutral salts in spin-labeled erythrocyte membranes |
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