Chromatographic separation of two ionic strength dependent conformations of valine transfer ribonucleic acid of Bacillus subtilis
The chromatographic profile of valyl tRNA Val of stationary phase B. subtilis W 23 on a salmine column shows three peaks I, II and III in the proportions 1/0.44/0.25. Exponential phase valyl tRNA Val shows only peaks I and II. The results obtained by rechromatography and by centrifugation on sucrose...
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| Veröffentlicht in: | Biochimie 1973, Vol.55 (2), p.127-134 |
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| creator | Heyman, T. Leidner, J. Menichi-Desseaux, B. |
| description | The chromatographic profile of valyl tRNA
Val of stationary phase B. subtilis W 23 on a salmine column shows three peaks I, II and III in the proportions 1/0.44/0.25. Exponential phase valyl tRNA
Val shows only peaks I and II.
The results obtained by rechromatography and by centrifugation on sucrose gradients show that valyl tRNA
Val eluted as peak III can be partially converted to a form I′, through a variation in the ionic strength. Form I and I′ give superimposable profiles.
Par chromatographie sur colonne de salmine, le valyl tARN
Val de
B. subtilis W 23 de phase stationnaire de croissance présente trois pics I, II et III dans les proportions 1/0,44/0,25. Le profil d'élution du valyl tARN
Val des bactéries de phase exponentielle ne comporte que les pics I et II.
Les résultats obtenus par rechromatographie et par centrifugation sur gradient de saccharose montrent que la forme III peut être partiellement convertie en une forme I′, superposable à I. Cette conversion se fait par simple variation de la force ionique. |
| doi_str_mv | 10.1016/S0300-9084(73)80384-0 |
| format | Article |
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Val of stationary phase B. subtilis W 23 on a salmine column shows three peaks I, II and III in the proportions 1/0.44/0.25. Exponential phase valyl tRNA
Val shows only peaks I and II.
The results obtained by rechromatography and by centrifugation on sucrose gradients show that valyl tRNA
Val eluted as peak III can be partially converted to a form I′, through a variation in the ionic strength. Form I and I′ give superimposable profiles.
Par chromatographie sur colonne de salmine, le valyl tARN
Val de
B. subtilis W 23 de phase stationnaire de croissance présente trois pics I, II et III dans les proportions 1/0,44/0,25. Le profil d'élution du valyl tARN
Val des bactéries de phase exponentielle ne comporte que les pics I et II.
Les résultats obtenus par rechromatographie et par centrifugation sur gradient de saccharose montrent que la forme III peut être partiellement convertie en une forme I′, superposable à I. Cette conversion se fait par simple variation de la force ionique.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/S0300-9084(73)80384-0</identifier><identifier>PMID: 4198186</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Amino Acyl-tRNA Synthetases - isolation & purification ; Bacillus subtilis - analysis ; Carbon Isotopes ; Centrifugation, Density Gradient ; Chromatography ; Nucleic Acid Conformation ; Protamines ; RNA, Transfer - isolation & purification ; Sucrose ; Valine</subject><ispartof>Biochimie, 1973, Vol.55 (2), p.127-134</ispartof><rights>1973</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c275t-ba09486d62e3c08d787d2166b745cc95efca0ee47687773bfaeca432495fa66a3</citedby><cites>FETCH-LOGICAL-c275t-ba09486d62e3c08d787d2166b745cc95efca0ee47687773bfaeca432495fa66a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0300-9084(73)80384-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4198186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heyman, T.</creatorcontrib><creatorcontrib>Leidner, J.</creatorcontrib><creatorcontrib>Menichi-Desseaux, B.</creatorcontrib><title>Chromatographic separation of two ionic strength dependent conformations of valine transfer ribonucleic acid of Bacillus subtilis</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>The chromatographic profile of valyl tRNA
Val of stationary phase B. subtilis W 23 on a salmine column shows three peaks I, II and III in the proportions 1/0.44/0.25. Exponential phase valyl tRNA
Val shows only peaks I and II.
The results obtained by rechromatography and by centrifugation on sucrose gradients show that valyl tRNA
Val eluted as peak III can be partially converted to a form I′, through a variation in the ionic strength. Form I and I′ give superimposable profiles.
Par chromatographie sur colonne de salmine, le valyl tARN
Val de
B. subtilis W 23 de phase stationnaire de croissance présente trois pics I, II et III dans les proportions 1/0,44/0,25. Le profil d'élution du valyl tARN
Val des bactéries de phase exponentielle ne comporte que les pics I et II.
Les résultats obtenus par rechromatographie et par centrifugation sur gradient de saccharose montrent que la forme III peut être partiellement convertie en une forme I′, superposable à I. Cette conversion se fait par simple variation de la force ionique.</description><subject>Amino Acyl-tRNA Synthetases - isolation & purification</subject><subject>Bacillus subtilis - analysis</subject><subject>Carbon Isotopes</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromatography</subject><subject>Nucleic Acid Conformation</subject><subject>Protamines</subject><subject>RNA, Transfer - isolation & purification</subject><subject>Sucrose</subject><subject>Valine</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v3CAQhlHUKtl8_IRInKr24BYMBnyqmlWbVorUQ5szwjDOEtngAk7UY_957d1VrjkxYp53RvMgdE3JR0qo-PSLMEKqlij-XrIPijDFK3KCNlQwVQmq2Bu0eUHO0HnOj4SQhtTtKTrltFVUiQ36t92lOJoSH5KZdt7iDJNJpvgYcOxxeY54Kdf_kiA8lB12MEFwEAq2MfQxjXs4r_STGXwAXJIJuYeEk-9imO0AS95Y71bmZimGYc44z13xg8-X6G1vhgxXx_cC3X_7-nv7vbr7eftj--WusrVsStUZ0nIlnKiBWaKcVNLVVIhO8sbatoHeGgLApVBSStb1BqzhrOZt0xshDLtA7w5zpxT_zJCLHn22MAwmQJyzVrQVitdyAZsDaFPMOUGvp-RHk_5qSvSqXu_V69Wrlkzv1Wuy5K6PC-ZuBPeSOrpe-p8PfViufPKQdLYeggXnE9iiXfSvbPgPjpeW6w</recordid><startdate>1973</startdate><enddate>1973</enddate><creator>Heyman, T.</creator><creator>Leidner, J.</creator><creator>Menichi-Desseaux, B.</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1973</creationdate><title>Chromatographic separation of two ionic strength dependent conformations of valine transfer ribonucleic acid of Bacillus subtilis</title><author>Heyman, T. ; Leidner, J. ; Menichi-Desseaux, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-ba09486d62e3c08d787d2166b745cc95efca0ee47687773bfaeca432495fa66a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Amino Acyl-tRNA Synthetases - isolation & purification</topic><topic>Bacillus subtilis - analysis</topic><topic>Carbon Isotopes</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromatography</topic><topic>Nucleic Acid Conformation</topic><topic>Protamines</topic><topic>RNA, Transfer - isolation & purification</topic><topic>Sucrose</topic><topic>Valine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heyman, T.</creatorcontrib><creatorcontrib>Leidner, J.</creatorcontrib><creatorcontrib>Menichi-Desseaux, B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heyman, T.</au><au>Leidner, J.</au><au>Menichi-Desseaux, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromatographic separation of two ionic strength dependent conformations of valine transfer ribonucleic acid of Bacillus subtilis</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>1973</date><risdate>1973</risdate><volume>55</volume><issue>2</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>The chromatographic profile of valyl tRNA
Val of stationary phase B. subtilis W 23 on a salmine column shows three peaks I, II and III in the proportions 1/0.44/0.25. Exponential phase valyl tRNA
Val shows only peaks I and II.
The results obtained by rechromatography and by centrifugation on sucrose gradients show that valyl tRNA
Val eluted as peak III can be partially converted to a form I′, through a variation in the ionic strength. Form I and I′ give superimposable profiles.
Par chromatographie sur colonne de salmine, le valyl tARN
Val de
B. subtilis W 23 de phase stationnaire de croissance présente trois pics I, II et III dans les proportions 1/0,44/0,25. Le profil d'élution du valyl tARN
Val des bactéries de phase exponentielle ne comporte que les pics I et II.
Les résultats obtenus par rechromatographie et par centrifugation sur gradient de saccharose montrent que la forme III peut être partiellement convertie en une forme I′, superposable à I. Cette conversion se fait par simple variation de la force ionique.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>4198186</pmid><doi>10.1016/S0300-9084(73)80384-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9084 |
| ispartof | Biochimie, 1973, Vol.55 (2), p.127-134 |
| issn | 0300-9084 1638-6183 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81968427 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acyl-tRNA Synthetases - isolation & purification Bacillus subtilis - analysis Carbon Isotopes Centrifugation, Density Gradient Chromatography Nucleic Acid Conformation Protamines RNA, Transfer - isolation & purification Sucrose Valine |
| title | Chromatographic separation of two ionic strength dependent conformations of valine transfer ribonucleic acid of Bacillus subtilis |
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