Effect of puromycin on protein and glycerolipid biosynthesis in isolated mucosal cells
The effect of puromycin on phosphatidylcholine and triacylglycerol synthesis was studied in isolated cells of rat intestinal mucosa using radioactive palmitate, glycerol, 2-hexadecylglycerol, and lysophosphatidylcholine as markers. Puromycin caused a 60–65% inhibition of phosphatidylcholine biosynth...
Gespeichert in:
| Veröffentlicht in: | Archives of biochemistry and biophysics 1973-06, Vol.156 (2), p.586-594 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 594 |
|---|---|
| container_issue | 2 |
| container_start_page | 586 |
| container_title | Archives of biochemistry and biophysics |
| container_volume | 156 |
| creator | O'Doherty, P.J.A. Yousef, I.M. Kuksis, A. |
| description | The effect of puromycin on phosphatidylcholine and triacylglycerol synthesis was studied in isolated cells of rat intestinal mucosa using radioactive palmitate, glycerol, 2-hexadecylglycerol, and lysophosphatidylcholine as markers. Puromycin caused a 60–65% inhibition of phosphatidylcholine biosynthesis but did not affect the formation of triacylglycerols. Under comparable conditions protein synthesis was inhibited 90–95% and glycoprotein synthesis 60–70%. The utilization of the various lipid precursors indicated that puromycin inhibited the biosynthesis of phosphatidylcholine via both the CDP-choline and the lysophosphatidylcholine pathways, without interfering with triacylglycerol synthesis from either phosphatidic acid or monoacylglycerol precursors. Since both phosphatidylcholines and proteins are involved in the assembly of chylomicrons, it is suggested that the effect of puromycin on chylomicron formation could be due to an inhibition of the biosynthesis of any one or all three of the membrane components: proteins, glycoproteins, and phosphatidylcholines. |
| doi_str_mv | 10.1016/0003-9861(73)90309-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_81957190</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003986173903093</els_id><sourcerecordid>81957190</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-746f234a390288648d13491e5a3a98a5f6ea1f56e49e54424e0b6031d4f9eff03</originalsourceid><addsrcrecordid>eNp9kE9LxDAQxYMo67r6DRRyEj1UkyZNk4sgy_oHFryo15BNJxppmzVphX57W3bx6GkG3ps3Mz-Ezim5oYSKW0IIy5QU9Kpk14owojJ2gOaUKJERJvkhmv9ZjtFJSl-EUMpFPkMzXlJZSj5H7yvnwHY4OLztY2gG61scWryNoYOxNW2FP-rBQgy13_oKb3xIQ9t9QvIJjwafQm06qHDT25BMjS3UdTpFR87UCc72dYHeHlavy6ds_fL4vLxfZ5YVZZeVXLicccMUyaUUXFaUcUWhMMwoaQonwFBXCOAKCs5zDmQjCKMVdwqcI2yBLne5473fPaRONz5NF5gWQp-0pKooqZqMfGe0MaQUwelt9I2Jg6ZETzj1xEpPrHQ51gmnZuPYxT6_3zRQ_Q3t-Y363U6H8ckfD1En66G1UPk4YtVV8P8v-AXra4RG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>81957190</pqid></control><display><type>article</type><title>Effect of puromycin on protein and glycerolipid biosynthesis in isolated mucosal cells</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>O'Doherty, P.J.A. ; Yousef, I.M. ; Kuksis, A.</creator><creatorcontrib>O'Doherty, P.J.A. ; Yousef, I.M. ; Kuksis, A.</creatorcontrib><description>The effect of puromycin on phosphatidylcholine and triacylglycerol synthesis was studied in isolated cells of rat intestinal mucosa using radioactive palmitate, glycerol, 2-hexadecylglycerol, and lysophosphatidylcholine as markers. Puromycin caused a 60–65% inhibition of phosphatidylcholine biosynthesis but did not affect the formation of triacylglycerols. Under comparable conditions protein synthesis was inhibited 90–95% and glycoprotein synthesis 60–70%. The utilization of the various lipid precursors indicated that puromycin inhibited the biosynthesis of phosphatidylcholine via both the CDP-choline and the lysophosphatidylcholine pathways, without interfering with triacylglycerol synthesis from either phosphatidic acid or monoacylglycerol precursors. Since both phosphatidylcholines and proteins are involved in the assembly of chylomicrons, it is suggested that the effect of puromycin on chylomicron formation could be due to an inhibition of the biosynthesis of any one or all three of the membrane components: proteins, glycoproteins, and phosphatidylcholines.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(73)90309-3</identifier><identifier>PMID: 4718784</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Carbon Isotopes ; Chromatography, Thin Layer ; Chylomicrons - biosynthesis ; Glucosamine - metabolism ; Glycerides - biosynthesis ; Glycerides - metabolism ; Glycerol - metabolism ; Glycoproteins - biosynthesis ; Intestinal Mucosa - cytology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Jejunum - drug effects ; Jejunum - metabolism ; Kinetics ; Leucine - metabolism ; Lysophosphatidylcholines - metabolism ; Male ; Palmitic Acids - metabolism ; Phosphatidylcholines - biosynthesis ; Protein Biosynthesis ; Puromycin - pharmacology ; Rats ; Triglycerides - biosynthesis ; Tritium</subject><ispartof>Archives of biochemistry and biophysics, 1973-06, Vol.156 (2), p.586-594</ispartof><rights>1973</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-746f234a390288648d13491e5a3a98a5f6ea1f56e49e54424e0b6031d4f9eff03</citedby><cites>FETCH-LOGICAL-c357t-746f234a390288648d13491e5a3a98a5f6ea1f56e49e54424e0b6031d4f9eff03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(73)90309-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4718784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>O'Doherty, P.J.A.</creatorcontrib><creatorcontrib>Yousef, I.M.</creatorcontrib><creatorcontrib>Kuksis, A.</creatorcontrib><title>Effect of puromycin on protein and glycerolipid biosynthesis in isolated mucosal cells</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The effect of puromycin on phosphatidylcholine and triacylglycerol synthesis was studied in isolated cells of rat intestinal mucosa using radioactive palmitate, glycerol, 2-hexadecylglycerol, and lysophosphatidylcholine as markers. Puromycin caused a 60–65% inhibition of phosphatidylcholine biosynthesis but did not affect the formation of triacylglycerols. Under comparable conditions protein synthesis was inhibited 90–95% and glycoprotein synthesis 60–70%. The utilization of the various lipid precursors indicated that puromycin inhibited the biosynthesis of phosphatidylcholine via both the CDP-choline and the lysophosphatidylcholine pathways, without interfering with triacylglycerol synthesis from either phosphatidic acid or monoacylglycerol precursors. Since both phosphatidylcholines and proteins are involved in the assembly of chylomicrons, it is suggested that the effect of puromycin on chylomicron formation could be due to an inhibition of the biosynthesis of any one or all three of the membrane components: proteins, glycoproteins, and phosphatidylcholines.</description><subject>Animals</subject><subject>Carbon Isotopes</subject><subject>Chromatography, Thin Layer</subject><subject>Chylomicrons - biosynthesis</subject><subject>Glucosamine - metabolism</subject><subject>Glycerides - biosynthesis</subject><subject>Glycerides - metabolism</subject><subject>Glycerol - metabolism</subject><subject>Glycoproteins - biosynthesis</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Jejunum - drug effects</subject><subject>Jejunum - metabolism</subject><subject>Kinetics</subject><subject>Leucine - metabolism</subject><subject>Lysophosphatidylcholines - metabolism</subject><subject>Male</subject><subject>Palmitic Acids - metabolism</subject><subject>Phosphatidylcholines - biosynthesis</subject><subject>Protein Biosynthesis</subject><subject>Puromycin - pharmacology</subject><subject>Rats</subject><subject>Triglycerides - biosynthesis</subject><subject>Tritium</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LxDAQxYMo67r6DRRyEj1UkyZNk4sgy_oHFryo15BNJxppmzVphX57W3bx6GkG3ps3Mz-Ezim5oYSKW0IIy5QU9Kpk14owojJ2gOaUKJERJvkhmv9ZjtFJSl-EUMpFPkMzXlJZSj5H7yvnwHY4OLztY2gG61scWryNoYOxNW2FP-rBQgy13_oKb3xIQ9t9QvIJjwafQm06qHDT25BMjS3UdTpFR87UCc72dYHeHlavy6ds_fL4vLxfZ5YVZZeVXLicccMUyaUUXFaUcUWhMMwoaQonwFBXCOAKCs5zDmQjCKMVdwqcI2yBLne5473fPaRONz5NF5gWQp-0pKooqZqMfGe0MaQUwelt9I2Jg6ZETzj1xEpPrHQ51gmnZuPYxT6_3zRQ_Q3t-Y363U6H8ckfD1En66G1UPk4YtVV8P8v-AXra4RG</recordid><startdate>197306</startdate><enddate>197306</enddate><creator>O'Doherty, P.J.A.</creator><creator>Yousef, I.M.</creator><creator>Kuksis, A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197306</creationdate><title>Effect of puromycin on protein and glycerolipid biosynthesis in isolated mucosal cells</title><author>O'Doherty, P.J.A. ; Yousef, I.M. ; Kuksis, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-746f234a390288648d13491e5a3a98a5f6ea1f56e49e54424e0b6031d4f9eff03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Animals</topic><topic>Carbon Isotopes</topic><topic>Chromatography, Thin Layer</topic><topic>Chylomicrons - biosynthesis</topic><topic>Glucosamine - metabolism</topic><topic>Glycerides - biosynthesis</topic><topic>Glycerides - metabolism</topic><topic>Glycerol - metabolism</topic><topic>Glycoproteins - biosynthesis</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Jejunum - drug effects</topic><topic>Jejunum - metabolism</topic><topic>Kinetics</topic><topic>Leucine - metabolism</topic><topic>Lysophosphatidylcholines - metabolism</topic><topic>Male</topic><topic>Palmitic Acids - metabolism</topic><topic>Phosphatidylcholines - biosynthesis</topic><topic>Protein Biosynthesis</topic><topic>Puromycin - pharmacology</topic><topic>Rats</topic><topic>Triglycerides - biosynthesis</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>O'Doherty, P.J.A.</creatorcontrib><creatorcontrib>Yousef, I.M.</creatorcontrib><creatorcontrib>Kuksis, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>O'Doherty, P.J.A.</au><au>Yousef, I.M.</au><au>Kuksis, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of puromycin on protein and glycerolipid biosynthesis in isolated mucosal cells</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1973-06</date><risdate>1973</risdate><volume>156</volume><issue>2</issue><spage>586</spage><epage>594</epage><pages>586-594</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The effect of puromycin on phosphatidylcholine and triacylglycerol synthesis was studied in isolated cells of rat intestinal mucosa using radioactive palmitate, glycerol, 2-hexadecylglycerol, and lysophosphatidylcholine as markers. Puromycin caused a 60–65% inhibition of phosphatidylcholine biosynthesis but did not affect the formation of triacylglycerols. Under comparable conditions protein synthesis was inhibited 90–95% and glycoprotein synthesis 60–70%. The utilization of the various lipid precursors indicated that puromycin inhibited the biosynthesis of phosphatidylcholine via both the CDP-choline and the lysophosphatidylcholine pathways, without interfering with triacylglycerol synthesis from either phosphatidic acid or monoacylglycerol precursors. Since both phosphatidylcholines and proteins are involved in the assembly of chylomicrons, it is suggested that the effect of puromycin on chylomicron formation could be due to an inhibition of the biosynthesis of any one or all three of the membrane components: proteins, glycoproteins, and phosphatidylcholines.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4718784</pmid><doi>10.1016/0003-9861(73)90309-3</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1973-06, Vol.156 (2), p.586-594 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81957190 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Carbon Isotopes Chromatography, Thin Layer Chylomicrons - biosynthesis Glucosamine - metabolism Glycerides - biosynthesis Glycerides - metabolism Glycerol - metabolism Glycoproteins - biosynthesis Intestinal Mucosa - cytology Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Jejunum - drug effects Jejunum - metabolism Kinetics Leucine - metabolism Lysophosphatidylcholines - metabolism Male Palmitic Acids - metabolism Phosphatidylcholines - biosynthesis Protein Biosynthesis Puromycin - pharmacology Rats Triglycerides - biosynthesis Tritium |
| title | Effect of puromycin on protein and glycerolipid biosynthesis in isolated mucosal cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T06%3A58%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20puromycin%20on%20protein%20and%20glycerolipid%20biosynthesis%20in%20isolated%20mucosal%20cells&rft.jtitle=Archives%20of%20biochemistry%20and%20biophysics&rft.au=O'Doherty,%20P.J.A.&rft.date=1973-06&rft.volume=156&rft.issue=2&rft.spage=586&rft.epage=594&rft.pages=586-594&rft.issn=0003-9861&rft.eissn=1096-0384&rft_id=info:doi/10.1016/0003-9861(73)90309-3&rft_dat=%3Cproquest_cross%3E81957190%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=81957190&rft_id=info:pmid/4718784&rft_els_id=0003986173903093&rfr_iscdi=true |