Stabilization of heterogeneous nuclear RNA by intercalating drugs
The effect of the intercalating drugs proflavine, ethidium and daunomycin on the rate of degradation of 14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of 14C-HnRNA to acid soluble products. The most striking effect was produce...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1973-06, Vol.52 (3), p.928-934 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Brinker, Jane M. Madore, H.Paul Bello, Leonard J. |
| description | The effect of the intercalating drugs proflavine, ethidium and daunomycin on the rate of degradation of
14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of
14C-HnRNA to acid soluble products. The most striking effect was produced by proflavine which promptly and completely stabilized
14C-HnRNA against degradation. Ethidium also produced complete stabilization after a delay of 30 to 60 min. Daunomycin decreased the rate of
14C-HnRNA degradation but did not alter the fraction of
14C-HnRNA which was ultimately degraded. The results are consistent with the view that base-paired sequences are present in HnRNA
in vivo
and play a role in the processing of HnRNA. |
| doi_str_mv | 10.1016/0006-291X(73)91026-7 |
| format | Article |
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14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of
14C-HnRNA to acid soluble products. The most striking effect was produced by proflavine which promptly and completely stabilized
14C-HnRNA against degradation. Ethidium also produced complete stabilization after a delay of 30 to 60 min. Daunomycin decreased the rate of
14C-HnRNA degradation but did not alter the fraction of
14C-HnRNA which was ultimately degraded. The results are consistent with the view that base-paired sequences are present in HnRNA
in vivo
and play a role in the processing of HnRNA.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(73)91026-7</identifier><identifier>PMID: 4710572</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acridines - pharmacology ; Amines - pharmacology ; Binding Sites ; Carbon Isotopes ; Carcinoma ; Cell Line ; Cycloheximide - pharmacology ; Dactinomycin - pharmacology ; Daunorubicin - pharmacology ; Drug Stability ; Ethidium - pharmacology ; Humans ; Kinetics ; Mouth Neoplasms ; Nucleic Acid Conformation ; Nucleic Acid Denaturation ; Phenanthridines - pharmacology ; RNA, Neoplasm - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1973-06, Vol.52 (3), p.928-934</ispartof><rights>1973</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-cb4fa2ceed1f467e9e233b83ca351640f96d425475fa4cbd3b9014cee55527323</citedby><cites>FETCH-LOGICAL-c357t-cb4fa2ceed1f467e9e233b83ca351640f96d425475fa4cbd3b9014cee55527323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(73)91026-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4710572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brinker, Jane M.</creatorcontrib><creatorcontrib>Madore, H.Paul</creatorcontrib><creatorcontrib>Bello, Leonard J.</creatorcontrib><title>Stabilization of heterogeneous nuclear RNA by intercalating drugs</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The effect of the intercalating drugs proflavine, ethidium and daunomycin on the rate of degradation of
14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of
14C-HnRNA to acid soluble products. The most striking effect was produced by proflavine which promptly and completely stabilized
14C-HnRNA against degradation. Ethidium also produced complete stabilization after a delay of 30 to 60 min. Daunomycin decreased the rate of
14C-HnRNA degradation but did not alter the fraction of
14C-HnRNA which was ultimately degraded. The results are consistent with the view that base-paired sequences are present in HnRNA
in vivo
and play a role in the processing of HnRNA.</description><subject>Acridines - pharmacology</subject><subject>Amines - pharmacology</subject><subject>Binding Sites</subject><subject>Carbon Isotopes</subject><subject>Carcinoma</subject><subject>Cell Line</subject><subject>Cycloheximide - pharmacology</subject><subject>Dactinomycin - pharmacology</subject><subject>Daunorubicin - pharmacology</subject><subject>Drug Stability</subject><subject>Ethidium - pharmacology</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Mouth Neoplasms</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Denaturation</subject><subject>Phenanthridines - pharmacology</subject><subject>RNA, Neoplasm - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcKeRA-rk4_dNBehiF9QFPwAbyGbna2R7W5NdoX6601t6dFTBt5nJjMPIccULijQ_BIA8pQp-n4m-bmiwPJU7pAhBQUpoyB2yXCL7JODED4BKBW5GpCBkBQyyYZk8tKZwtXux3SubZK2Sj6wQ9_OsMG2D0nT2xqNT54fJ0mxTFwTQ2vqSDezpPT9LBySvcrUAY8274i83d68Xt-n06e7h-vJNLU8k11qC1EZZhFLWolcokLGeTHm1vCM5gIqlZeCZUJmlRG2KHmhgIrIZ1nGJGd8RE7Xcxe-_eoxdHrugsW6Nn-b6jFV8WqACIo1aH0bgsdKL7ybG7_UFPTKnF5p0SstWnL9Zy4WI3Kymd8Xcyy3TRtVMb9a5xiP_HbodbAOG4ul82g7Xbbu_w9-ASlPfT8</recordid><startdate>19730608</startdate><enddate>19730608</enddate><creator>Brinker, Jane M.</creator><creator>Madore, H.Paul</creator><creator>Bello, Leonard J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19730608</creationdate><title>Stabilization of heterogeneous nuclear RNA by intercalating drugs</title><author>Brinker, Jane M. ; Madore, H.Paul ; Bello, Leonard J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-cb4fa2ceed1f467e9e233b83ca351640f96d425475fa4cbd3b9014cee55527323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Acridines - pharmacology</topic><topic>Amines - pharmacology</topic><topic>Binding Sites</topic><topic>Carbon Isotopes</topic><topic>Carcinoma</topic><topic>Cell Line</topic><topic>Cycloheximide - pharmacology</topic><topic>Dactinomycin - pharmacology</topic><topic>Daunorubicin - pharmacology</topic><topic>Drug Stability</topic><topic>Ethidium - pharmacology</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Mouth Neoplasms</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Denaturation</topic><topic>Phenanthridines - pharmacology</topic><topic>RNA, Neoplasm - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brinker, Jane M.</creatorcontrib><creatorcontrib>Madore, H.Paul</creatorcontrib><creatorcontrib>Bello, Leonard J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brinker, Jane M.</au><au>Madore, H.Paul</au><au>Bello, Leonard J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stabilization of heterogeneous nuclear RNA by intercalating drugs</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1973-06-08</date><risdate>1973</risdate><volume>52</volume><issue>3</issue><spage>928</spage><epage>934</epage><pages>928-934</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The effect of the intercalating drugs proflavine, ethidium and daunomycin on the rate of degradation of
14C-labeled heterogeneous nuclear RNA (HnRNA) in KB cells was studied. All three drugs decreased the rate of degradation of
14C-HnRNA to acid soluble products. The most striking effect was produced by proflavine which promptly and completely stabilized
14C-HnRNA against degradation. Ethidium also produced complete stabilization after a delay of 30 to 60 min. Daunomycin decreased the rate of
14C-HnRNA degradation but did not alter the fraction of
14C-HnRNA which was ultimately degraded. The results are consistent with the view that base-paired sequences are present in HnRNA
in vivo
and play a role in the processing of HnRNA.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4710572</pmid><doi>10.1016/0006-291X(73)91026-7</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1973-06, Vol.52 (3), p.928-934 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_81921000 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acridines - pharmacology Amines - pharmacology Binding Sites Carbon Isotopes Carcinoma Cell Line Cycloheximide - pharmacology Dactinomycin - pharmacology Daunorubicin - pharmacology Drug Stability Ethidium - pharmacology Humans Kinetics Mouth Neoplasms Nucleic Acid Conformation Nucleic Acid Denaturation Phenanthridines - pharmacology RNA, Neoplasm - metabolism |
| title | Stabilization of heterogeneous nuclear RNA by intercalating drugs |
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