DNA CONTENT OF PURIFIED PREPARATIONS OF MOUSE PURKINJE NEURONS ISOLATED BY A VELOCITY SEDIMENTATION TECHNIQUE

DNA CONTENT OF PURIFIED PREPARATIONS OF MOUSE PURKINJE NEURONS ISOLATED BY A VELOCITY SEDIMENTATION TECHNIQUE

— The DNA content of mouse Purkinje neurons was investigated employing a biochemical approach. Material for the biochemical assay was provided by means of a sedimentation velocity separation technique which yields bulk quantities of well‐preserved Purkinje perikarya in a high degree of purity. The s...

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Veröffentlicht in:Journal of neurochemistry 1973-03, Vol.20 (3), p.651-657
Hauptverfasser: Cohen, J., Mareš, V., Lodin, Z.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Nucleus - analysis
Cerebellum - analysis
Cerebellum - cytology
Cytological Techniques
DNA - analysis
Female
Histocytochemistry
Male
Mice
Mice, Inbred Strains
Microscopy, Phase-Contrast
Purkinje Cells - analysis
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container_title Journal of neurochemistry
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creator Cohen, J.
Mareš, V.
Lodin, Z.
description — The DNA content of mouse Purkinje neurons was investigated employing a biochemical approach. Material for the biochemical assay was provided by means of a sedimentation velocity separation technique which yields bulk quantities of well‐preserved Purkinje perikarya in a high degree of purity. The same amount of DNA/cell was recorded for mixed cerebellar cell somata (7·6 ± 0±2 pg/cell), as for the Purkinje perikarya enriched fractions (7±2 & 0·2 pg/cell). No evidence could be found for the existence of a tetraploid DNA complement in mouse Purkinje neurons despite indications to the contrary from a parallel cytophotometric study.
doi_str_mv 10.1111/j.1471-4159.1973.tb00025.x
format Article
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source MEDLINE; Access via Wiley Online Library
subjects Animals
Cell Nucleus - analysis
Cerebellum - analysis
Cerebellum - cytology
Cytological Techniques
DNA - analysis
Female
Histocytochemistry
Male
Mice
Mice, Inbred Strains
Microscopy, Phase-Contrast
Purkinje Cells - analysis
title DNA CONTENT OF PURIFIED PREPARATIONS OF MOUSE PURKINJE NEURONS ISOLATED BY A VELOCITY SEDIMENTATION TECHNIQUE
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