The effect of free fatty acid and bilirubin on a dye-binding assay for albumin with and without the presence of detergent

1. The effect of oleate and of bilirubin on the assay of albumin by a fluorescent dye-binding technique has been studied. 2. Using a solution of the dye 1-anilino-naphthalene-8-sulphonic acid (ANSA) in phosphate buffer and saline, competition of both oleate and bilirubin for dye-binding sites was ob...

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