A method to improve contrast in high resolution electron microscopy of ultrathin tissue sections

A method to improve contrast in high resolution electron microscopy of ultrathin tissue sections

The general background granularity observed in out-of-focus electron micrographs is discussed as a phase contrast effect, that prevents higher resolution than about 25 Å on low contrast specimens as ultrathin tissue sections. The problem of improving the specimen resolution when dealing with ultrath...

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Veröffentlicht in:Experimental cell research 1956-06, Vol.10 (3), p.657-664
1. Verfasser: SJOSTRAND, F S
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Electrons
Microscopy
Microscopy, Electron
Old Medline
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description The general background granularity observed in out-of-focus electron micrographs is discussed as a phase contrast effect, that prevents higher resolution than about 25 Å on low contrast specimens as ultrathin tissue sections. The problem of improving the specimen resolution when dealing with ultrathin sections consists of improving the contrast so that in-focus pictures may be used. A method of mounting the sections on formvar nets is described, which makes it possible to obtain pictures of sections, which are partially not supported by a formvar film. The reduced diffuse scattering of the electrons when passing through these areas means a drastic increase of contrast.
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subjects Electrons
Microscopy
Microscopy, Electron
Old Medline
title A method to improve contrast in high resolution electron microscopy of ultrathin tissue sections
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