Epidermal Intercellular Staining with Fluorescein-Conjugated Phytohemagglutinins
Fluorescein-conjugated phytohemagglutinins, Concanavalin A and Phytohemagglutinin P were used to stain frozen-cut sections of human skin to determine the presence and distribution of carbohydrate-containing substances in the epidermis. An intercellular staining pattern was observed which appeared id...
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| Veröffentlicht in: | Journal of investigative dermatology 1973-02, Vol.60 (2), p.61-66 |
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| creator | Nieland, Michael L. |
| description | Fluorescein-conjugated phytohemagglutinins, Concanavalin A and Phytohemagglutinin P were used to stain frozen-cut sections of human skin to determine the presence and distribution of carbohydrate-containing substances in the epidermis. An intercellular staining pattern was observed which appeared identical to that found in various forms of pemphigus. The intercellular staining could be inhibited, or abolished, by α-methyl-D-mannopyranoside or α-methyI-D-glucopyranoside in the case of Concanavalin A, and by N-acetyl-D-galactosamine in the case of Phytohemagglutinin P, but not by a variety of other monosaccharides tested. A similar pattern of staining could be produced in vivo in mouse and guinea-pig skin by intradermal injection of fluorescein-conjugated Concanavalin A. Staining with fluorescein isothiocyanate alone produced a staining pattern which was a negative image of that seen with the fluorescein-conjugated phytohemagglutinins, namely cytoplasmic staining, but absent intercellular staining. |
| doi_str_mv | 10.1111/1523-1747.ep12724137 |
| format | Article |
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An intercellular staining pattern was observed which appeared identical to that found in various forms of pemphigus. The intercellular staining could be inhibited, or abolished, by α-methyl-D-mannopyranoside or α-methyI-D-glucopyranoside in the case of Concanavalin A, and by N-acetyl-D-galactosamine in the case of Phytohemagglutinin P, but not by a variety of other monosaccharides tested. A similar pattern of staining could be produced in vivo in mouse and guinea-pig skin by intradermal injection of fluorescein-conjugated Concanavalin A. Staining with fluorescein isothiocyanate alone produced a staining pattern which was a negative image of that seen with the fluorescein-conjugated phytohemagglutinins, namely cytoplasmic staining, but absent intercellular staining.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12724137</identifier><identifier>PMID: 4120828</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Membrane ; Cell Nucleus ; Concanavalin A ; Fluoresceins ; Humans ; Lectins ; Microscopy, Fluorescence ; Monosaccharides - analysis ; Pemphigus - pathology ; Protein Binding ; Skin - analysis ; Staining and Labeling</subject><ispartof>Journal of investigative dermatology, 1973-02, Vol.60 (2), p.61-66</ispartof><rights>1973 The Williams & Wilkins Co.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-e6177b287caba48a1683987973d3ae53fc6030e646fd20409bbbe382be23b5ab3</citedby><cites>FETCH-LOGICAL-c425t-e6177b287caba48a1683987973d3ae53fc6030e646fd20409bbbe382be23b5ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4120828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nieland, Michael L.</creatorcontrib><title>Epidermal Intercellular Staining with Fluorescein-Conjugated Phytohemagglutinins</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Fluorescein-conjugated phytohemagglutinins, Concanavalin A and Phytohemagglutinin P were used to stain frozen-cut sections of human skin to determine the presence and distribution of carbohydrate-containing substances in the epidermis. An intercellular staining pattern was observed which appeared identical to that found in various forms of pemphigus. The intercellular staining could be inhibited, or abolished, by α-methyl-D-mannopyranoside or α-methyI-D-glucopyranoside in the case of Concanavalin A, and by N-acetyl-D-galactosamine in the case of Phytohemagglutinin P, but not by a variety of other monosaccharides tested. A similar pattern of staining could be produced in vivo in mouse and guinea-pig skin by intradermal injection of fluorescein-conjugated Concanavalin A. Staining with fluorescein isothiocyanate alone produced a staining pattern which was a negative image of that seen with the fluorescein-conjugated phytohemagglutinins, namely cytoplasmic staining, but absent intercellular staining.</description><subject>Cell Membrane</subject><subject>Cell Nucleus</subject><subject>Concanavalin A</subject><subject>Fluoresceins</subject><subject>Humans</subject><subject>Lectins</subject><subject>Microscopy, Fluorescence</subject><subject>Monosaccharides - analysis</subject><subject>Pemphigus - pathology</subject><subject>Protein Binding</subject><subject>Skin - analysis</subject><subject>Staining and Labeling</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1973</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcKevG3N127SiyCl1ULBggreQpKdtin7UZOs0n9vl5Z6cy4D877vMPMgdEvwkOzrgWSUpURwMYQtoYJywsQZ6p_G56iPMaUpxfTzEl2FsMGY5DyTPdTjhGJJZR8tJltXgK90mczqCN5CWbal9slb1K529Sr5cXGdTMu28RAsuDodN_WmXekIRbJY72KzhkqvVmUbO3-4RhdLXQa4OfYB-phO3scv6fz1eTZ-mqeW0yymkBMhDJXCaqO51CSXbCTFSLCCacjY0uaYYch5viwo5nhkjAEmqQHKTKYNG6D7w96tb75aCFFVLnTX6xqaNihJJM8EYXsjPxitb0LwsFRb7yrtd4pg1YFUHTHVEVN_IPexu-P-1lRQnEJHcn96rWPr4aRn3WNZl3886LCH8O3Aq2Ad1BYK58FGVTTu_wN-AZACjYo</recordid><startdate>197302</startdate><enddate>197302</enddate><creator>Nieland, Michael L.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197302</creationdate><title>Epidermal Intercellular Staining with Fluorescein-Conjugated Phytohemagglutinins</title><author>Nieland, Michael L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-e6177b287caba48a1683987973d3ae53fc6030e646fd20409bbbe382be23b5ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1973</creationdate><topic>Cell Membrane</topic><topic>Cell Nucleus</topic><topic>Concanavalin A</topic><topic>Fluoresceins</topic><topic>Humans</topic><topic>Lectins</topic><topic>Microscopy, Fluorescence</topic><topic>Monosaccharides - analysis</topic><topic>Pemphigus - pathology</topic><topic>Protein Binding</topic><topic>Skin - analysis</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nieland, Michael L.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nieland, Michael L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidermal Intercellular Staining with Fluorescein-Conjugated Phytohemagglutinins</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1973-02</date><risdate>1973</risdate><volume>60</volume><issue>2</issue><spage>61</spage><epage>66</epage><pages>61-66</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><abstract>Fluorescein-conjugated phytohemagglutinins, Concanavalin A and Phytohemagglutinin P were used to stain frozen-cut sections of human skin to determine the presence and distribution of carbohydrate-containing substances in the epidermis. An intercellular staining pattern was observed which appeared identical to that found in various forms of pemphigus. The intercellular staining could be inhibited, or abolished, by α-methyl-D-mannopyranoside or α-methyI-D-glucopyranoside in the case of Concanavalin A, and by N-acetyl-D-galactosamine in the case of Phytohemagglutinin P, but not by a variety of other monosaccharides tested. A similar pattern of staining could be produced in vivo in mouse and guinea-pig skin by intradermal injection of fluorescein-conjugated Concanavalin A. Staining with fluorescein isothiocyanate alone produced a staining pattern which was a negative image of that seen with the fluorescein-conjugated phytohemagglutinins, namely cytoplasmic staining, but absent intercellular staining.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4120828</pmid><doi>10.1111/1523-1747.ep12724137</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of investigative dermatology, 1973-02, Vol.60 (2), p.61-66 |
| issn | 0022-202X 1523-1747 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Cell Membrane Cell Nucleus Concanavalin A Fluoresceins Humans Lectins Microscopy, Fluorescence Monosaccharides - analysis Pemphigus - pathology Protein Binding Skin - analysis Staining and Labeling |
| title | Epidermal Intercellular Staining with Fluorescein-Conjugated Phytohemagglutinins |
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